dbCAN-seq: a database of CAZyme sequence and annotation

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CAZyme Information: MGYG000004381_00072

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Basic Information help

Species

Lachnospira sp900772425

Lineage

Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnospira; Lachnospira sp900772425

CAZyme ID

MGYG000004381_00072

CAZy Family

CBM48

CAZyme Description

1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
823	MGYG000004381_1\|CGC1	93332.89	4.7652

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004381	2725945	MAG	Israel	Asia

Gene Location

Start: 73433; End: 75904 Strand: -

Full Sequence Download help

Enzyme Prediction help

No EC number prediction in MGYG000004381_00072.

CAZyme Signature Domains help

Family	Start	End	Evalue	family coverage
GH13	278	576	2e-97	0.9900332225913622
CBM48	128	214	2.3e-17	0.9078947368421053

CDD Domains download full data without filtering help

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	0.0	13	729	5	725	1,4-alpha-glucan branching protein GlgB.
PRK12313	PRK12313	0.0	110	729	9	629	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	7.79e-158	103	726	1	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	1.28e-140	14	719	508	1214	glycogen branching enzyme; Provisional
cd11322	AmyAc_Glg_BE	5.52e-139	221	611	7	402	Alpha amylase catalytic domain found in the Glycogen branching enzyme (also called 1,4-alpha-glucan branching enzyme). The glycogen branching enzyme catalyzes the third step of glycogen biosynthesis by the cleavage of an alpha-(1,4)-glucosidic linkage and the formation a new alpha-(1,6)-branch by subsequent transfer of cleaved oligosaccharide. They are part of a group called branching enzymes which catalyze the formation of alpha-1,6 branch points in either glycogen or starch. This group includes proteins from bacteria, eukaryotes, and archaea. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.

CAZyme Hits help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACR71776.1	1.50e-242	1	756	1	726
QNM06418.1	1.73e-186	5	757	5	764
BCN32019.1	3.20e-186	1	746	1	750
QHQ61835.1	1.56e-185	5	729	5	735
QQQ92601.1	2.34e-184	1	725	1	730

PDB Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR1_A	3.52e-143	2	729	18	775	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR0_A	9.80e-143	2	729	18	775	Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	2.72e-142	2	729	18	775	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	3.83e-142	2	729	18	775	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	5.38e-142	2	729	18	775	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q21M30	3.45e-142	14	726	9	726	1,4-alpha-glucan branching enzyme GlgB OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) OX=203122 GN=glgB PE=3 SV=1
Q8DLB8	8.32e-140	14	726	8	749	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q1AZ86	9.37e-140	14	729	7	721	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q1D654	1.32e-139	13	725	13	731	1,4-alpha-glucan branching enzyme GlgB OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=glgB PE=3 SV=1
Q114I1	2.17e-139	14	742	8	774	1,4-alpha-glucan branching enzyme GlgB OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000017	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM Annotations help

There is no transmembrane helices in MGYG000004381_00072.