CAZyme Information: MGYG000004389_00551

Basic Information

• Species: CAG-302 sp900543825
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; CAG-302; CAG-302 sp900543825
• CAZyme ID: MGYG000004389_00551
• CAZy Family: CBM50
• CAZyme Description: Cell division suppressor protein YneA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
409	MGYG000004389_1|CGC3	45721.56	6.3133

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004389	1101000	MAG	Israel	Asia

• Gene Location: Start: 556575; End: 557804 Strand: +

Full Sequence      

MNNNEDRAVRKVIIDPGHGGTDAGATGNNLLEKDYNLLISKYMYDRFKQLGVPVAITRDS
DTTLSPTDRVNTILNKFGNSSDVILISNHVNSGGGEGAEVIYALRNKDTLAKRILENIGA
TGQTTRKYYQRRLPSDTSKDYYFIHRNTGNLEPLIVEYGFIDNTKDVEFLKENYEELAEA
VISAVANYIGVPYTPPEGLITNTYVVQKGDTLYSIANKLGTTVSELKKENNLTSNTLQIG
EVLRIPTKEIYEEEENIYIVKKGDSLYSIANKYNTTVDELKRINNLTSNILSIGQVLKLP
SDKASNVEKEENTISYTVQKGDSLYSIARKYNTTIDRIKDLNNLTTNLLSIGQVLLIPTD
TNLETTYTVQKGDSLYSIAKKYNTTVNRLKQLNNLTSNLLSIGQILIVR

Enzyme Prediction     

No EC number prediction in MGYG000004389_00551.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CBM50	316	358	4.7e-17	0.975
CBM50	258	300	4.4e-16	0.975

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK06347	PRK06347	9.38e-38	203	409	332	592	1,4-beta-N-acetylmuramoylhydrolase.
cd02696	MurNAc-LAA	1.05e-32	11	186	1	172	N-acetylmuramoyl-L-alanine amidase or MurNAc-LAA (also known as peptidoglycan aminohydrolase, NAMLA amidase, NAMLAA, Amidase 3, and peptidoglycan amidase; EC 3.5.1.28) is an autolysin that hydrolyzes the amide bond between N-acetylmuramoyl and L-amino acids in certain cell wall glycopeptides. These proteins are Zn-dependent peptidases with highly conserved residues involved in cation co-ordination. MurNAc-LAA in this family is one of several peptidoglycan hydrolases (PGHs) found in bacterial and bacteriophage or prophage genomes that are involved in the degradation of the peptidoglycan. In Escherichia coli, there are five MurNAc-LAAs present: AmiA, AmiB, AmiC and AmiD that are periplasmic, and AmpD that is cytoplasmic. Three of these (AmiA, AmiB and AmiC) belong to this family, the other two (AmiD and AmpD) do not. E. coli AmiA, AmiB and AmiC play an important role in cleaving the septum to release daughter cells after cell division. In general, bacterial MurNAc-LAAs are members of the bacterial autolytic system and carry a signal peptide in their N-termini that allows their transport across the cytoplasmic membrane. However, the bacteriophage MurNAc-LAAs are endolysins since these phage-encoded enzymes break down bacterial peptidoglycan at the terminal stage of the phage reproduction cycle. As opposed to autolysins, almost all endolysins have no signal peptides and their translocation through the cytoplasmic membrane is thought to proceed with the help of phage-encoded holin proteins. The amidase catalytic module is fused to another functional module (cell wall binding module or CWBM) either at the N- or C-terminus, which is responsible for high affinity binding of the protein to the cell wall.
PRK06347	PRK06347	1.52e-28	194	357	396	591	1,4-beta-N-acetylmuramoylhydrolase.
pfam01520	Amidase_3	1.69e-28	12	185	1	172	N-acetylmuramoyl-L-alanine amidase. This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls.
COG0860	AmiC	1.27e-26	3	190	36	229	N-acetylmuramoyl-L-alanine amidase [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QVK20078.1	2.02e-78	12	300	2	284
QIC07671.1	7.57e-52	6	241	150	376
AYB39952.1	7.95e-52	6	199	150	338
QIC05530.1	1.26e-51	6	198	78	265
ACL69398.1	1.79e-51	188	408	130	357

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5J72_A	3.94e-15	3	170	445	616	ChainA, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630],5J72_B Chain B, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630]
4B8V_A	9.52e-13	258	408	44	216	ChainA, Extracellular Protein 6 [Fulvia fulva],4B9H_A Chain A, Extracellular Protein 6 [Fulvia fulva]
5EMI_A	3.52e-10	12	189	7	178	ChainA, Cell wall hydrolase/autolysin [Nostoc punctiforme PCC 73102]
4UZ2_A	1.03e-09	258	301	5	48	Crystalstructure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_B Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_C Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_D Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ3_A Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8],4UZ3_B Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8],4UZ3_C Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8]
4XCM_A	1.28e-08	258	301	5	48	Crystalstructure of the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4XCM_B Crystal structure of the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O07532	3.08e-32	202	408	92	350	Peptidoglycan endopeptidase LytF OS=Bacillus subtilis (strain 168) OX=224308 GN=lytF PE=1 SV=2
O31852	1.01e-30	203	408	28	268	D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS OS=Bacillus subtilis (strain 168) OX=224308 GN=cwlS PE=1 SV=1
P54421	3.90e-26	206	357	30	192	Probable peptidoglycan endopeptidase LytE OS=Bacillus subtilis (strain 168) OX=224308 GN=lytE PE=1 SV=1
Q49UX4	1.44e-23	258	397	29	182	N-acetylmuramoyl-L-alanine amidase sle1 OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=sle1 PE=3 SV=1
Q8CMN2	4.85e-23	203	358	28	191	N-acetylmuramoyl-L-alanine amidase sle1 OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=sle1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000044	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004389_00551.