CAZyme Information: MGYG000004390_01082

Basic Information

• Species: Prevotella sp002265625
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp002265625
• CAZyme ID: MGYG000004390_01082
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
692	MGYG000004390_15|CGC1	77156.59	7.6736

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004390	3321071	MAG	Israel	Asia

• Gene Location: Start: 40930; End: 43008 Strand: +

Full Sequence      

MKNFILSAALLVATSASVSAKSNAPKVMANGYPYTQVPFTSVKMVQNSFWGARLKAAREV
TVPLAFSKCESEHRYKNFDMAAYTLKHPGHAGLQTPEWDVAKFMGFSFDDTDVYKTIEGA
SYILQTYPDKKLKAYIDSVLNVVGAAQEPDGYLYTARTINPEHPHAWSGKKRWEKEEDLS
HELYNLGHMVDAACAHYQATGSHKFLDIARRYADCVCREVGSGSGQATVVPGHQIAEMAL
ARLYTLTGEKRYLDEAKYLLDFRGKTHIRQEYSQSHKPILNQDEAVGHAVRAGYMYAGIA
DIAALTQDSAYIKVIDRIWQNIISKKVYLTGGVGARHEGEAFGKNYELPNMTAYNETCAA
ISMVYLYERMFLLHGESKYVDCLERTLYNGVISGMSMDGGRFFYPNPLSSDGKYAFNADN
NTTRQPWFGCACCPSNLSRFIPSVPGYLYGVKDNNIYVNLFAANTSTIQVGGKDVVLEET
TQYPWDGDIQIRVVKSAAKNANMLVRIPGWVQNQVLPSDLYKYSDQERPAYTVTVNGKKV
EADLAANKGYLPVKNIRKGDVVRIHFDMPVRTVVANQNVKDDEGRVAVERGPIVYCAEAA
DNQGEPVLRAVMNKQPKFTVVNDYKVDNTETAGAPSFAVKAITTDAQMLEENADGVAVKQ
QKLTLIPYYAWNHRGAGEMNVWFVQSLKMLDK

Enzyme Prediction     

No EC number prediction in MGYG000004390_01082.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	45	596	7.1e-200	0.9942748091603053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	41	596	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	4.59e-153	37	682	12	584	Uncharacterized conserved protein, DUF1680 family [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNT67047.1	0.0	5	692	9	691
BCS84603.1	0.0	31	688	13	659
VEH14288.1	0.0	10	682	5	666
AVM58298.1	8.63e-309	26	682	23	654
AVM52620.1	3.07e-306	1	682	1	654

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	6.93e-288	30	682	3	622	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	6.41e-132	45	682	18	644	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	7.35e-127	45	682	18	644	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.30e-119	102	681	67	654	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.76e-119	102	681	67	654	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	7.11e-119	102	681	67	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000415	0.998787	0.000167	0.000224	0.000199	0.000179

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004390_01082.