CAZyme Information: MGYG000004420_00986

Basic Information

• Species: RUG841 sp900313795
• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; RUG841; RUG841 sp900313795
• CAZyme ID: MGYG000004420_00986
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
716		78846.39	10.0362

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004420	2033021	MAG	Israel	Asia

• Gene Location: Start: 43163; End: 45313 Strand: +

Full Sequence      

MAKKKGSKKGKQKKDNPLFSFIKASLVTIVAIGLAFVIMLKVFLMTQPPIPNLQNFKPNQ
VTQIYSEDGELIKTFTAYKFEPTHIKDVPKNLVNALIATEDKNFYKHQGYDLFGIVRSTF
ANISARRTVQGASTITQQLSRILFLSNERSFNRKIKEIIIASRIEKSLTKDQILEMYLNN
VYLGSGAYGAAGAAATYFNKPLKDLTLAECALIAGLPQAPSVYSPFNNKDLALKRRAKVL
DRMYKMKYITKDEMKQAKDEPLKLSQVVGARTLNRAPYFVDYVMQELEELGFDEQDISQG
GYKITTTLNYKVQKAAEEAVNRNLASWGLSGSKNQAAVFSFSPVDGKIIAYVGGKNYAQS
QYDRVTQAVRPPGSSFKPFVYATAVYKGWSPNDIIEDTPFSIGTWSPRNYGSKYRGKIPL
YKALALSSNVAAVRLIDDVGIKPVIIMARELGITTPLANDSTIALGSNGVKLYDMTIAFG
VFANGGFKVKPFSVLRVETARGKTIYSAEKVSKTKVLHFETAGAVNAMLEQVIAAGTGAA
ARIGVPQAGKTGTTDDCKDAWFVGYTPDISTGVWVGNDDNTKLPGITGGTLPAIIWRDTM
KVTAESEKNAKFPYPKFELKASTKVPQSEITEKVESQAADPDKAAHEEIQKRLQEQENAL
KEQMNKPAAPTPRKPNYETQLEPVRLTPAEPSKNFNENLNNQKAQNSGGGSQGRFL

Enzyme Prediction     

No EC number prediction in MGYG000004420_00986.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	68	244	8.2e-67	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	0.0	21	616	8	731	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	81	601	3	526	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	0.0	26	641	20	634	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	1.45e-119	56	601	109	680	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	3.60e-103	32	626	10	584	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR38680.1	1.15e-276	9	691	22	712
AEE14353.1	1.07e-147	48	613	36	602
AME04620.1	1.30e-147	54	601	52	605
AKT54746.1	1.84e-147	54	601	52	605
ANR71459.1	3.66e-147	51	618	49	618

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	2.63e-96	53	596	12	715	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	1.29e-95	63	600	22	712	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5U2G_A	8.48e-78	48	508	8	566	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3ZG8_B	5.30e-70	164	582	3	437	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5FGZ_A	6.53e-68	58	588	142	681	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P70997	7.40e-102	60	616	53	620	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
Q9ZCE9	5.60e-99	48	615	35	728	Penicillin-binding protein 1A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=mrcA PE=3 SV=1
Q68VU2	1.05e-98	48	615	35	728	Penicillin-binding protein 1A OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=mrcA PE=3 SV=1
Q92G78	2.28e-98	48	615	35	728	Penicillin-binding protein 1A OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=mrcA PE=3 SV=1
Q4UK08	4.44e-98	48	615	35	728	Penicillin-binding protein 1A OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000033	0.000008	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
21	43