CAZyme Information: MGYG000004423_00867

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; ML615J-28; CAG-698; DUOT01
• CAZyme ID: MGYG000004423_00867
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1177		132506.66	5.4483

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004423	1147030	MAG	Israel	Asia

• Gene Location: Start: 6049; End: 9582 Strand: -

Full Sequence      

MKKVNEMTLKEKLGQLIIAGFDGYEYDEHLRTLIEDYKVGNIILFTRNIKDIHQLSALNR
KIYNEILKNTGNSPLISIDQEGGMVTRIMDGATFCPGNMTLTATNNSDNAYIIGKIMGDE
LTHLGINMNLAPSLDVNNNPDNPVIGVRSYSDNPKVVSEYGNKFIKGLQEKGVIATAKHF
PGHGDTNVDSHLGLPMINHTLEELEKVELVPFRSAIENGVDAIMSAHIVFSEIDKSGLPG
TLSKTILTDLLRNKLNFKGLIVSDCMQMKAIDDQYTTERGTVMGIKAGLDIACISHSLDK
QVGALKALEEAVETGEIDISIIDEKVKRILHYKELTRKKLESDFLSFSDDEIVNYFKNLN
NQEIASKIVDESLTIFKGKEFVQTGKTLVIAATPFAQTIAEDKLDTRNIVDMLREFVKTV
DAETLDLKQEDYSDLINKAKNYDQIVVCSYNANNYPNQVKLINDLNNLGKDLYVISTRNP
YDYLVLDEIDNLACLYEYTKNSVGTIMRYLSGMIKPKGKFPINANNKLNVSASIYVGLED
YSLKNNIKYLHLLKENGIDSVFVSAHMPEISSTFTNELKEILNECKKLNIKVIIDVNKNK
LLEMEKEGTLAGIYSLRLDYGFSNNEIVEMIKKGYNIELNASVVNKETMEYLKANGVDLS
NFRISHNFYPKKYTGLSYEFVLEKNKYFKSLGCYVVLYIPSHHQHRPPMYEGLPTLEAHR
DAPLTEILSEAKILGCNEVCFGDAFCSEEEIKYATTFDSGIIQIPVIIKKECDENVVKNL
LKVQYNRIDETEYFVRSSVRSTNIKPFNTVNRYVKDVTIDNELFKRYQGEICIMKKNIEA
DERVNVVGKCNCNEFLINNIKAGQKFKLVIDQVIAEEAIIGIDAGGTKTKVALIDANKEI
VYSAIGGSGSPAVDPNALNTIYNLIKEVYDLYNDKYNIKAVVMGVSGVYTIENRHEFLEK
IENDFNTTAILESDALLALYSIVQDKYEEGLLILAGTGTAIMGLNKDKSFLASGWGQLLN
EHGSAYACVKDYVCEMIDDYEDKGEFTEFGKKLMQHLGYTDLQEFKKLFYRHSKDEVASS
SKFFYEEAKKGDKEAQKWLYHNGEMLADNALDAIRHLKMSKRVVIGFRGGFASNVHYVQE
GVIDTLKKNGIEPIVVKGDLDPIYGSFYMAKRKGALC

Enzyme Prediction     

No EC number prediction in MGYG000004423_00867.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH170	532	869	1.3e-91	0.9885714285714285
GH3	72	293	4.3e-65	0.9537037037037037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00933	Glyco_hydro_3	2.26e-100	8	330	1	314	Glycosyl hydrolase family 3 N terminal domain.
COG1472	BglX	2.73e-98	7	377	1	342	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
COG3589	COG3589	2.72e-64	533	871	7	359	Uncharacterized protein [Function unknown].
pfam19200	DUF871_N	4.39e-58	533	752	4	231	DUF871 N-terminal domain. This family consists of several conserved hypothetical proteins from bacteria and archaea. The function of this family is unknown.
PRK05337	PRK05337	1.92e-56	23	343	10	319	beta-hexosaminidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QVK18519.1	7.77e-165	1	522	1	517
AWI66943.1	9.67e-159	1	526	1	520
VEU80306.1	2.35e-156	4	522	6	521
VEU82113.1	7.39e-155	4	522	6	521
CCV65417.1	1.08e-153	4	522	5	520

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6K5J_A	8.81e-105	7	521	11	528	Structureof a glycoside hydrolase family 3 beta-N-acetylglucosaminidase from Paenibacillus sp. str. FPU-7 [Paenibacillaceae]
4ZM6_A	2.35e-91	5	550	5	553	Aunique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain GH3 beta-N-acetylglucosaminidase [Rhizomucor miehei CAU432],4ZM6_B A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain GH3 beta-N-acetylglucosaminidase [Rhizomucor miehei CAU432]
3BMX_A	2.75e-84	4	535	39	628	Beta-N-hexosaminidase(YbbD) from Bacillus subtilis [Bacillus subtilis],3BMX_B Beta-N-hexosaminidase (YbbD) from Bacillus subtilis [Bacillus subtilis],3NVD_A Structure of YBBD in complex with pugnac [Bacillus subtilis],3NVD_B Structure of YBBD in complex with pugnac [Bacillus subtilis]
3LK6_A	7.19e-84	4	535	13	602	ChainA, Lipoprotein ybbD [Bacillus subtilis],3LK6_B Chain B, Lipoprotein ybbD [Bacillus subtilis],3LK6_C Chain C, Lipoprotein ybbD [Bacillus subtilis],3LK6_D Chain D, Lipoprotein ybbD [Bacillus subtilis]
4GYJ_A	1.55e-83	4	535	43	632	Crystalstructure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYJ_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYK_A Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168],4GYK_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P40406	1.51e-83	4	535	39	628	Beta-hexosaminidase OS=Bacillus subtilis (strain 168) OX=224308 GN=nagZ PE=1 SV=1
P48823	8.31e-54	7	371	16	416	Beta-hexosaminidase A OS=Pseudoalteromonas piscicida OX=43662 GN=cht60 PE=1 SV=1
Q6LJ30	2.09e-43	23	340	10	314	Beta-hexosaminidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=nagZ PE=3 SV=1
C4LEY6	2.77e-41	13	293	1	277	Beta-hexosaminidase OS=Tolumonas auensis (strain DSM 9187 / TA4) OX=595494 GN=nagZ PE=3 SV=1
Q5QUZ5	3.41e-41	13	330	1	306	Beta-hexosaminidase OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) OX=283942 GN=nagZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000043	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004423_00867.