CAZyme Information: MGYG000004440_00989

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; RFN20; CAG-449; CAG-449
• CAZyme ID: MGYG000004440_00989
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
265	MGYG000004440_10|CGC2	31618.72	9.4604

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004440	1765228	MAG	Israel	Asia

• Gene Location: Start: 62926; End: 63723 Strand: +

Full Sequence      

MNILVTLNKNYLPYLISLIRSINDNNKNTLFDFYIFSNDITLKDIKKYDNYLDKTNKYFI
INIPKEDFKNAPITKRYPYEMYYRIFASKYLPSSLDRILYLDPDIIVKGNLSNLYNINFE
GNYYAGCTNIKKILKRINQIKNGAEKDNEYINTGVLMINLTLLRDHLNEEAIYNFIEDKK
YLLTLPDQDVISTIYGKKILLLNTFIYNLSEKMIKIHNLSQKEDKIDLDWVEKNTIIIHY
LGKNKPWKENYKGILLPYYKKYEVK

Enzyme Prediction     

No EC number prediction in MGYG000004440_00989.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	2	250	2.5e-45	0.8754863813229572

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	1.82e-56	1	248	1	247	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
pfam01501	Glyco_transf_8	7.56e-28	2	250	1	251	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
COG1442	RfaJ	9.65e-28	1	252	3	249	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
PRK15171	PRK15171	8.55e-19	1	249	26	272	lipopolysaccharide 3-alpha-galactosyltransferase.
cd00505	Glyco_transf_8	2.63e-13	5	248	6	245	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARC85976.1	4.57e-80	1	262	1	265
AFL99655.1	7.24e-78	1	265	1	268
CDX04293.1	1.03e-77	1	265	1	268
ACL19350.1	5.85e-77	1	265	1	268
BAE85862.1	3.65e-76	1	265	15	282

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3TZT_A	1.76e-44	3	262	8	267	Thestructure of a protein in glycosyl transferase family 8 from Anaerococcus prevotii. [Anaerococcus prevotii DSM 20548],3TZT_B The structure of a protein in glycosyl transferase family 8 from Anaerococcus prevotii. [Anaerococcus prevotii DSM 20548]
1SS9_A	8.71e-11	61	247	62	252	ChainA, alpha-1,4-galactosyl transferase [Neisseria meningitidis]
1G9R_A	7.86e-09	61	247	62	252	ChainA, GLYCOSYL TRANSFERASE [Neisseria meningitidis],1GA8_A Chain A, GALACTOSYL TRANSFERASE LGTC [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P19817	1.98e-19	63	260	88	283	Lipopolysaccharide 1,2-glucosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaJ PE=3 SV=4
P27129	2.79e-19	1	264	28	286	Lipopolysaccharide 1,2-glucosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaJ PE=3 SV=2
A0A0H2URH2	5.10e-15	11	247	13	229	Glycosyltransferase GlyF OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyF PE=3 SV=1
P19816	1.47e-13	2	247	29	272	Lipopolysaccharide 1,3-galactosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaI PE=3 SV=4
P27128	1.63e-10	19	249	47	275	Lipopolysaccharide 1,3-galactosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaI PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000075	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004440_00989.