CAZyme Information: MGYG000004443_01570

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Coprobacteraceae
• CAZyme ID: MGYG000004443_01570
• CAZy Family: GH2
• CAZyme Description: Evolved beta-galactosidase subunit alpha

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1339		151597.75	5.7187

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004443	2430140	MAG	Israel	Asia

• Gene Location: Start: 8282; End: 12301 Strand: -

Full Sequence      

MKKHWIIFFLFLSLSGYSQQSILEGFSYGDVDAPRGDEWQSPSSLALNKEQPRAYFFSFA
DMESARKVLPQYSDYWMSLNGPWYFYWVGNPEERPVEFYQPDFDVSRWDVVEVPMNWNVV
GIQKDGSLKYGVPVYANQPVIFQHQVKVGDWKGGVMRTPPQDWTTYKHRNEVGSYRRNFS
LPENWKDREVYLNFDGVSSFFYLWVNGHYVGFSKNSRNTASFNITRYLNKKGDNTVAVEV
YRNSDGSFLEAQDMFRLPGIFRTVSLSSTPQVQVRDFYVVPDLDSQYKNGSIEISADIRN
LSKKKIKEYSLSYTLYENELYSDKTAVVENGTVSVGVKEIEKGESSLVLARMDVDTPRKW
SAEAPYRYTLVGQLKDKKGRVVETVSVGFGFRKVEIKDTPAEQDEFGLAGRYFYINGKPV
KLKGVNRQEIDPERGHAITHELMENDIKLLKRANLNHVRNSHYSNDPYWYYLCDKYGIYL
EDEANIESHQYYYGEASLSHVPEFKAAHVARVMELVHAHVNAPSIVIWSLGNEAGPGENF
VAAYQAIKAFDTSRPVQYERNNDIVDMGSNQYPSVDWVRGAVKGKYAIKYPFHISEYAHS
MGNAVGNLQDYWDAIESTNFFCGAAIWDWVDQSLYYYDKQTGTRYMAYGGDFGDKPNSGM
FCMNGILFPDHSPKPQYYEVKKVMQDVGVKAVDMERGIVEIFNKRYFTDLGNLDVRWALY
EDGKEIADGMLEPGEIAARQSKQVTVPYISEKQKENSEYFLKIEFLLNRDEPWAEKGYVQ
MEEQLAVKEPTQKPALVSVADGNPLKISEEQNCTVVSGSNFQVRFDHQNGSIHSLKYGDQ
TIIEAGNGPKLDALRAPTDNDNWAYQQWFAKGLHNLKHQAVLNTVYTDKNGSAVLSFTVV
SQAPHAATITGGSSGRYKITEHEDRPFGPQDFKFVTDQIWTVYRDGSIELQSSITSNDEG
VVLPRLGYEMVLPERLQHYTYYGRGPVNNYNDRKTASFIEQYTSTVKEQFVLFPKPQSMG
NREDVRWCALTDETGHGVMFIATDRLSASALPWSALEMTLAPHPYQLPPSSGTHLHLDAG
VTGLGGNSCGQGGPLEPDRVKASAHRMGFIIRPAETSRFTETACVSASGQTPLMVKRDRR
GELTLSSSQKGAVLCYQVGENGKPQVYDAPVNMRDGGTIIAWYQENPELKVTYTFEKIEN
IPMEVVYASSQETGGESAANLVDNDPATIWHTMYSVTVAQYPHWVDFDAGEQRLVKGFVY
LPRQDGSGNGDIKEYKLQVSDDGKTWSDAVAQGSFAPDKKAQRVILQKPVKARYVRFTAL
SSQRGDDFASGAEFSVLAE

Enzyme Prediction     

No EC number prediction in MGYG000004443_01570.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	70	999	6.3e-212	0.9680851063829787
CBM32	1208	1335	8e-28	0.9193548387096774

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09525	lacZ	0.0	39	1086	17	999	beta-galactosidase.
PRK10340	ebgA	0.0	39	1113	4	999	cryptic beta-D-galactosidase subunit alpha; Reviewed
COG3250	LacZ	1.33e-129	69	997	5	804	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	8.34e-84	413	687	8	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
smart01038	Bgal_small_N	1.33e-76	817	1111	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRO23809.1	0.0	13	1339	14	1348
AIM35762.1	0.0	23	1339	25	1342
QQD14684.1	0.0	23	1339	31	1347
CDT15944.1	0.0	25	1339	33	1347
BAD50119.1	0.0	17	1339	18	1341

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	2.43e-194	38	1115	3	983	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	2.50e-194	38	1115	4	984	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3DEC_A	1.14e-173	38	1115	6	998	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3BGA_A	1.77e-161	38	1092	9	980	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	4.39e-128	38	1087	15	996	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56307	1.37e-193	38	1115	4	984	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
O52847	6.92e-188	38	1091	19	1010	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
A1SWB8	6.84e-161	38	1087	12	1008	Beta-galactosidase OS=Psychromonas ingrahamii (strain 37) OX=357804 GN=lacZ PE=3 SV=1
P06864	1.43e-153	37	1126	2	1012	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4
Q6LL68	1.64e-145	38	1113	11	1027	Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.004343	0.674476	0.320460	0.000238	0.000244	0.000231

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004443_01570.