CAZyme Information: MGYG000004446_00224

Basic Information

• Species: CAG-465 sp000433335
• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-465; CAG-465; CAG-465 sp000433335
• CAZyme ID: MGYG000004446_00224
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1854		215852.49	8.7515

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004446	1127366	MAG	Israel	Asia

• Gene Location: Start: 232342; End: 237906 Strand: -

Full Sequence      

MKNKLRNIFIEADQNYKIIKETYKILEKSYEMKLPIHSAGQWILDNMYILEQEHNEICEA
KKSLRHVRLPVIKTSEGSKYISIYYLAYELVEQNTGYIDQNIIKNCLNSHQKTSYLTSEE
LNLFILMIKIGLLKFIARVATNICHAQMQKIIVENLLKEENSDLLRICENVEKEFLVFRN
FKTYILEESKIKNANTAFVEYMAYRLKELGKKGEKYYELLKSESEKIGFTIDEAIIKEHM
EVAKTTDYMGRAILSYKQLIGINFREIFEAVNKVDETLKNGDYTGEFEKCDYKTKTRYRN
YIIKLAKKYKVSELYVAKKAVDCSVKYKKHVGFFLCSDEKYLLKKEMGKSYLSEIFYNKV
VDKIKPHIYILSILILAFEFTVLFAVDIFNFGNTALNIIFYIIAFCFFTEISEKLINYFI
RKNMIPEVLPRFDFAKTIDEKYPTYVIMPTVISSIEKLDEMINKMEITYLANRSENMYYM
LIGDCISSNKEHIMIDDTIVAYAKEKLDKLNEKYEMPHKLFNFIYRKRVYCKSEAAYMGW
ERKRGAISEFNKLVLGKLNYAEINKKFYLIYNDIIKAKYAITIDEDTNLSLNTAKDLVAI
IAHPLNRPKLSKNGKIVTSGYGIITPAVGLDIESANKTLFSKIFGGFGGLDIYTTAVANV
YQDVFGEAIFCGKGVYDIELFEKLLGNEIPENLVLSHDLLEGSYMRTGFASDIEVQDGFP
KNYISYMKRNHRWYRGDMQIIRWLLSPKSPINSLSKWKIFDNLRRPTVNLMGMIAIILSL
FVSMHFFAFTVLIVFLAINFGTLLGMIDKLIFGIHRHTKELQYIPIIHGFDADLLTMSLN
LINLPYTAYMCLDAFSKSLFRMIFSKRHLLEWTTADSLDKKTKKSVMYYYLSMIINVIVG
LFVILYPSPNIYGNIGLIIYKDSIGIFFLLGPLFSYLLGKNYLLNRRKRLTPSQDKEILE
IGKRTWNFFDTLMTKTNNYLPTDNFQSSRRNKIVSRTSSTNIGFGILAIIDAYDLKYITY
EELIEKLTNIYRTIDSLEKWHGHLYNWYNIKTLEPLRPRFISTVDSGNFVASLYVAKTFF
EELLGARDYDRLDSIKYDQRVVRLLELNNILIEDTDFTVLYNTSRNLFSIGYAIESGELL
DSYYDMLMSECRTTSLIAISRRQVTSKHWFALSRNLTKLDGYKGLVSWSGTAFEYFMPYI
FSKSYEHTLIDQSLFFTLYSNIKYGRINGTPWGISESAFAVKDNDLNYQYQAFGVSWLGL
KRGLNNYLVVAPYASVLMLPFSPQRVYKNLMKLKALGAYDSYGFYESIDYTKQNLDHGDD
MKIVKTYMAHHQGMSLCAINNYINHGILQNRFTENKDIKATEILLKEREKMRAKPTINST
DRLNTFKQRNIDKYTTSVSSLTYSYKDSENNDKQKFEVAFLNGYGMSAIYTSNGGDYIKY
KDKIVNKQRYKDIKTTGNYVVLTDKCTGDIISTSDISKDQNKPYIKKVSFVSDLSNIVTY
IQTKELETTTTTFISPEYNMEIKKVSMYNNSNTRREILINTYIELAMTDYMTNVVHPSFS
NLQIETYFDEQLETLIASKRKKNENDKEFYVYAKLIGIDLDIGVETEKLKIIQKQDEAYN
ENIVKYPLWPVLSYRATIILDPHEKQEFFYVVGASDSKYKISNAVVNLDYTEINKQLKLT
CELNSVVSRYLRLKPGRASIYNNIIRDVLFGAKELDSSEFWNENISQSMLWKYSISGDLP
IMIVNVEDIESSGIVDEVIRFMDYVKNRSIDIDIIMLIDEEIEKYGPIYTYVKTRLDRAV
YSNWTRGNIYLLNKNFLLQNEVKLLKFLSTKTINDVTEFLIPDKESSNNNEVKQ

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT84	1142	1354	1.1e-84	0.986046511627907

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10091	Glycoamylase	1.63e-25	1142	1355	1	214	Putative glucoamylase. The structure of UniProt:Q5LIB7 has an alpha/alpha toroid fold and is similar structurally to a number of glucoamylases. Most of these structural homologs are glucoamylases, involved in breaking down complex sugars (e.g. starch). The biologically relevant state is likely to be monomeric. The putative active site is located at the centre of the toroid with a well defined large cavity.
cd11753	GH94N_ChvB_NdvB_2_like	3.65e-25	1348	1658	1	314	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	2.95e-15	1431	1653	18	247	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.
COG3459	COG3459	7.84e-04	1604	1761	2	161	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
cd04191	Glucan_BSP_MdoH	0.002	694	739	202	253	Glucan_BSP_MdoH catalyzes the elongation of beta-1,2 polyglucose chains of glucan. Periplasmic Glucan Biosynthesis protein MdoH is a glucosyltransferase that catalyzes the elongation of beta-1,2 polyglucose chains of glucan, requiring a beta-glucoside as a primer and UDP-glucose as a substrate. Glucans are composed of 5 to 10 units of glucose forming a highly branched structure, where beta-1,2-linked glucose constitutes a linear backbone to which branches are attached by beta-1,6 linkages. In Escherichia coli, glucans are located in the periplasmic space, functioning as regulator of osmolarity. It is synthesized at a maximum when cells are grown in a medium with low osmolarity. It has been shown to span the cytoplasmic membrane.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCX34529.1	1.85e-308	8	1825	64	1861
AEV69563.1	2.19e-305	13	1825	72	2074
ADV79124.1	6.55e-296	13	1825	72	2040
ABY94171.1	6.55e-296	13	1825	72	2040
AEM79409.1	9.26e-296	13	1825	73	2041

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	2.67e-186	14	1786	64	1943	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
367	389
394	416
766	788
792	814
885	907
917	939
993	1015