CAZyme Information: MGYG000004449_00324

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-508; CAG-245
• CAZyme ID: MGYG000004449_00324
• CAZy Family: GT51
• CAZyme Description: Monofunctional glycosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
234	MGYG000004449_9|CGC1	27023.05	5.218

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004449	1509316	MAG	Israel	Asia

• Gene Location: Start: 10293; End: 10997 Strand: +

Full Sequence      

MMKILKRIIFIVVLISMSIALLFVGNGYDMYKKSIEETSVEDKIAEIKDKENYTNFSELP
QMYKNAVIAVEDHRFYKHNGIDIIAIGRSAFNDIKTMSFVEGGSTVTQQLAKNIYFTQEK
KIERKIAEVFMAFEIEKNCDKDEILELYLNTSYFGDGYYTPKEACRGYFNKELNEMTDYE
CILLAGIPNAPSVYAPTKNPDLAKQRQRQVIDKMVKYKCLTQDEANEILSQNNN

Enzyme Prediction     

No EC number prediction in MGYG000004449_00324.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	44	215	9.7e-61	0.9717514124293786

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00912	Transgly	2.08e-76	53	215	15	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
TIGR02074	PBP_1a_fam	1.96e-74	53	227	4	178	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	1.38e-67	1	231	8	257	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.28e-64	1	227	1	243	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK13481	PRK13481	2.15e-44	2	231	1	231	glycosyltransferase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM09777.1	7.59e-91	7	231	19	243
QRP41835.1	2.16e-86	26	230	28	232
ASN93505.1	2.16e-86	26	230	28	232
QJU22158.1	2.16e-86	26	230	28	232
ANU46909.1	1.76e-85	26	230	28	232

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2OQO_A	1.28e-41	53	231	22	200	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]
3NB6_A	5.11e-41	53	231	22	200	Crystalstructure of Aquifex aeolicus peptidoglycan glycosyltransferase in complex with Methylphosphoryl Neryl Moenomycin [Aquifex aeolicus]
5ZZK_A	2.28e-34	42	233	48	245	ChainA, Monofunctional glycosyltransferase [Staphylococcus aureus subsp. aureus Mu50]
3HZS_A	2.47e-34	42	233	12	209	ChainA, Monofunctional glycosyltransferase [Staphylococcus aureus subsp. aureus MW2]
6FTB_A	2.54e-34	42	233	13	210	Staphylococcusaureus monofunctional glycosyltransferase in complex with moenomycin [Staphylococcus aureus MW2]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38050	5.82e-42	19	214	28	230	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q5FAC7	6.00e-42	53	231	70	248	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=mrcA PE=3 SV=3
O05131	6.00e-42	53	231	70	248	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae OX=485 GN=mrcA PE=1 SV=3
P0A0Z5	6.00e-42	53	231	70	248	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1
P0A0Z6	6.00e-42	53	231	70	248	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.893468	0.097012	0.008267	0.000243	0.000171	0.000849

TMHMM  Annotations     

start	end
7	24