CAZyme Information: MGYG000004461_00426

Basic Information

• Lineage: Bacteria; Verrucomicrobiota; Lentisphaeria; Victivallales; UBA1829
• CAZyme ID: MGYG000004461_00426
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
550	MGYG000004461_2|CGC1	63122.82	9.5452

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004461	4658877	MAG	Israel	Asia

• Gene Location: Start: 159304; End: 160956 Strand: +

Full Sequence      

MKKMLILLSVLLILGYIAPLGVRELFSPDETRYGEIGREMRVLNDWVVPHLNGVHYFEKP
VMGYWFFAASQEMFGENAFAVRFPCMIMTLLSAAALFFFSRRFSAGGSREALLTVFAFLL
MPLVFGLATTSTLDPIFSSFLTLGMILFYCAVHSSSWKRAGFLFACGAAFGAAFLTKGFL
AIVLPGISIFAYLLWSRRFREIFLLPWIPLLGFLLISLPWAWEIHRAAPDFWPYFVYDEH
IKRFLEKEQHPEPFYYFIPVLLGGLGYLLFYLPTAVQGFRKTVFKEDFFRYNFCWIVLPF
LFLSASTGKLPTYILPCFAPLAVLFSAGFYKAFIEEKKVAAFNLATQIFGGAVLLAAVVL
AVIQLSNPEWVLFNKAESWKWVAVLIVAVVLAVLLYFASSEKKVWDKLAYLCMAMIPVLF
IYHFIIPERVEARKTVCSLVDKVRPKVGNDVKLIAYRHPFHGVCWGFQREDVYLYRFGGE
LTRGLGFMKREDRILDIEKTNEFILENRNKGGVLLFLPNKVYQEDKGKLPKPRWVESNTK
DRKGYSAVFF

Enzyme Prediction     

No EC number prediction in MGYG000004461_00426.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	3	493	3e-107	0.9092592592592592

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK13279	arnT	3.39e-111	16	532	19	536	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
COG1807	ArnT	1.28e-44	16	332	21	339	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
pfam13231	PMT_2	2.49e-13	59	222	2	159	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	1.70e-09	16	235	16	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSH42095.1	2.29e-117	1	517	1	524
QSH42096.1	2.72e-113	2	496	4	504
CBX27700.1	3.16e-100	2	538	6	550
BCL61706.1	1.65e-94	16	541	18	547
AVM46372.1	6.23e-88	7	494	6	499

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	9.79e-32	9	484	39	513	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
C5BDQ8	1.09e-87	8	517	10	519	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Edwardsiella ictaluri (strain 93-146) OX=634503 GN=arnT PE=3 SV=1
A8FRR0	1.53e-85	13	532	17	527	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Shewanella sediminis (strain HAW-EB3) OX=425104 GN=arnT PE=3 SV=1
A8GDR9	2.53e-81	6	536	10	541	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Serratia proteamaculans (strain 568) OX=399741 GN=arnT PE=3 SV=1
B2VBI7	3.38e-81	6	517	8	519	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99) OX=465817 GN=arnT PE=3 SV=1
B4ETL9	1.48e-78	6	538	12	541	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase 2 OS=Proteus mirabilis (strain HI4320) OX=529507 GN=arnT2 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.404068	0.593504	0.000860	0.000420	0.000493	0.000644

TMHMM  Annotations     

start	end
5	22
78	100
112	131
136	153
160	174
178	195
202	224
254	276
288	307
312	334
341	363
378	400
407	426