CAZyme Information: MGYG000004461_02181

Basic Information

• Lineage: Bacteria; Verrucomicrobiota; Lentisphaeria; Victivallales; UBA1829
• CAZyme ID: MGYG000004461_02181
• CAZy Family: GH39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
983		113133.36	9.5908

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004461	4658877	MAG	Israel	Asia

• Gene Location: Start: 53642; End: 56593 Strand: +

Full Sequence      

MIKYIILLLLSATTALDAITLCDFSKEHSPVPVWQGKRLEQSAVFENGGLRVRWNTALNG
ICIFAEPWKKSEPLEEFKEANCYVTLEIPEHSHLTSFRVRFIDSQKEVFQWGTATDFRKP
GTYRLSIPMTQKNFQGSFRGNNDKKIDFPIRFYSCVATALKNSGEASILVRKIEYEPTLP
DSELNLVKFDLETGTVPRVLRKGEEGKLKLLLRNPGRDPLPCRAKIEFRDFFDQVLSEEA
LLTLPARGTLEYKPQTRLNRLGHWTVHLRLESPDGSAFSERKRSLAYMVPAGPGKLYKEG
FIFGVCIPGWYDEAIFPKEAETAALCGASALRLNFRWRELEQKPDSWNLSRLNRIMEEYE
KRGMEVMPILSNPPVWARKNTPNSLPVFKEWRKYTSFLFRKYGDRIRYWEIWNEPDLTSF
CEFSAPEYLELQKIAREEQKKWAPNSKLLSGGFAHAYPDNNKDGFQEYVLAHGKKYFDIH
AFHGHGGFKPYRRQVEQFLLPMRKRNGVSAPWYANETAISACGIGEKKQAETLFKKFLFS
WSAGAIGYNWYNLRNNGFRGNDPEHHYGLVTFDFYPKPVYVVYNTLATFFRNKKWIRRHT
KGNAIWILEFEGGGDRIIALWNDSPENTPDEVAIFRSDAKSVETIDLMGNLRKRSSGTLY
EHQIRQEPQILLFKECSSLQYLGSSVKGWIAGGAVPGKPAEWNLSLFNPSGKARTVHLKL
NHGPGYQVEPVPEKVVLYPGKRKRIRAKVSVSEMKKNHENALDLKLSSENEHIQIRFPMK
WTVFIPRDKQADQWDFILRNREQVHALFDADPGNLHRLWSGPEDLSAKIRMNIKEGIWTI
QISVTDDKHVQTHQGVNVWKGDNIQLAFRISGQSSLWTAGLTLLANGKPELFLWDLPSEF
LKQNPLQSWKLEARRSGTRTEYEVKIPLSSIGVTPELLKLGIRFNVLINDNDGFGREGWI
QITDGIAGYRSSDPYPLLVFETR

Enzyme Prediction     

No EC number prediction in MGYG000004461_02181.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH39	346	625	8.3e-20	0.7262180974477959

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd09621	CBM9_like_5	2.47e-48	794	980	1	188	DOMON-like type 9 carbohydrate binding module. Family 9 carbohydrate-binding modules (CBM9) play a role in the microbial degradation of cellulose and hemicellulose (materials found in plants). The domain has previously been called cellulose-binding domain. The polysaccharide binding sites of CBMs with available 3D structure have been found to be either flat surfaces with interactions formed by predominantly aromatic residues (tryptophan and tyrosine), or extended shallow grooves. CBM9 domains found in this uncharacterized heterogeneous subfamily are often located at the C-terminus of longer proteins and may co-occur with various other functional domains such as glycosyl hydrolases. The CBM9 module in these architectures may be involved in binding to carbohydrates.
cd09619	CBM9_like_4	8.15e-13	818	958	29	167	DOMON-like type 9 carbohydrate binding module. Family 9 carbohydrate-binding modules (CBM9) play a role in the microbial degradation of cellulose and hemicellulose (materials found in plants). The domain has previously been called cellulose-binding domain. The polysaccharide binding sites of CBMs with available 3D structure have been found to be either flat surfaces with interactions formed by predominantly aromatic residues (tryptophan and tyrosine), or extended shallow grooves. CBM9 domains found in this uncharacterized heterogeneous subfamily are often located at the C-terminus of longer proteins and may co-occur with various other domains.
cd00241	DOMON_like	1.59e-05	819	960	12	156	Domon-like ligand-binding domains. DOMON-like domains can be found in all three kindgoms of life and are a diverse group of ligand binding domains that have been shown to interact with sugars and hemes. DOMON domains were initially thought to confer protein-protein interactions. They were subsequently found as a heme-binding motif in cellobiose dehydrogenase, an extracellular fungal oxidoreductase that degrades both lignin and cellulose, and in ethylbenzene dehydrogenase, an enzyme that aids in the anaerobic degradation of hydrocarbons. The domain interacts with sugars in the type 9 carbohydrate binding modules (CBM9), which are present in a variety of glycosyl hydrolases, and it can also be found at the N-terminus of sensor histidine kinases.
pfam01229	Glyco_hydro_39	0.003	339	458	67	204	Glycosyl hydrolases family 39.
pfam06452	CBM9_1	0.004	913	975	114	174	Carbohydrate family 9 binding domain-like. CBM9_1 is a C-terminal domain on bacterial xylanase proteins, and it is tandemly repeated in a number of family-members. The CBM9 module binds to amorphous and crystalline cellulose and a range of soluble di- and monosaccharides as well as to cello- and xylo- oligomers of different degrees of polymerization. Comparison of the glucose and cellobiose complexes during crystallisation reveals surprising differences in binding of these two substrates by CBM9-2. Cellobiose was found to bind in a distinct orientation from glucose, while still maintaining optimal stacking and electrostatic interactions with the reducing end sugar.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVM46276.1	3.28e-164	163	981	306	1137
AVM47149.1	2.83e-156	127	981	434	1307
AVM44759.1	6.60e-143	208	981	179	965
AHF94342.1	1.98e-140	46	981	79	1033
AHF90198.1	1.32e-123	74	980	79	1005

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JVK_A	1.49e-09	304	649	107	473	Structuralinsights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2. [Bacteroides cellulosilyticus],5JVK_B Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2. [Bacteroides cellulosilyticus],5JVK_C Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2. [Bacteroides cellulosilyticus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000367	0.998983	0.000175	0.000155	0.000142	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004461_02181.