CAZyme Information: MGYG000004462_00045

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000004462_00045
• CAZy Family: PL11
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1284	MGYG000004462_1|CGC2	138633.51	4.7503

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004462	2469543	MAG	Israel	Asia

• Gene Location: Start: 59451; End: 63305 Strand: +

Full Sequence      

MAHAGTSVPALLLLAALAVPSTVPAQRITSVAGRGVVATSRTSRGVTSWLVSWRRLAQDP
EGACYNLYSSVGGSSWTKVNGELLSVSNYSPASIADGTRFCVTVTENGKEGAKSTPFTYR
KPITDNAYLFIDFRTCGSPLDVTTTTAKFAWPCDLNNDGVTDYVIDRRRLSGTGSDERED
SEGGFDRTMTDKLEAYLGDGTYLWTIELGPNVQIDEGHNDMVTVGDFDGDGRAEVCVQVS
DGTQFADGKYIRYAGPLFGDDIKADGLSADSDGDGIINYNAASVRRTPPQYLAVIDGMTG
AQKAITPLPQPVDSKWTYTRDKCDSKGNSYYHSSDVGYMRLAGHMASAYLDGVHQSVVYE
YCYRLKEGGEHHYFTTAFGFFPDGDAARNFGPLWSVSHIDNGLGLFHQLRIADVDGDGRD
EVANGVCVIDHDGRNIIRTGIQHGDRFRLGDIDPERKGLECFAIQQNSDDMLGQVLYEAG
TGVHIKDWYCREAFDVGRGQCMDVDPSRKGLEMWSLMGNLYDCKGNLAWEQSNTNYLLSP
AEDIWWDGELDREQLTTSSGAQRQIIRKFDRSYKGTNLKHFAEESGEKARCWSSRFDMVA
DCIGDWREEVMQRWYEDSNGDGTDDCVGVVFYTTDRPTDVDNIHWLMQDGAYHGQTTCRG
YVQSPMTGFYLGYDMPRPPLPPYITADRTWGASDSEWRQTEGSVLFPLTGNKDIRLSGRV
TPSDVYFIVPSGSDYNVGGGGSIAATGRLWKSGDGTVTVNADISAVGGIIVSEGTLCLNG
KVASDIILRARGRLTGDYVMAEGCAVTREEDYLYVDGQPTVDTSDLVIDRDTRLDFTEPA
AVLDRKIILNADLTVNTASGVVLAMKRQITGPGRLIKTGKGQVNLMFADSSTAAANDWTG
GLVINEGTVCQGTCLTTFGAGPITVKRGTIRQFYNTAISTQPSFDYDVCVADEADSLDFI
LSGRTTVTGKWSGRGTVCFAQTYSRGDVKGDWTDFKGVMTCRKNDEESTYDALRLTQPLK
MPDGTLRLPAGMTAACSSANAANTIGELHGGGRWSNGMWTVGGRKFGSETVVGEARTWTF
NRHQSGEVIADELTDMSGLYLHYGPGSARSLTALPYNAVLSFGDGTRQSVSVVALSGGAD
CLPAISANDPTAGSPVAGDFCFGVNVGRPGTLYVQVAPDGVATSGKMCLTCNGSVVDTAT
GTGADGIKELSYTCGSPGTYFFTSGKAFRIMGVRFSPAATSSAIVSMPSSTDVTATSSVY
NLVGCKVGPEYKGIVIQNGRKIVR

Enzyme Prediction     

No EC number prediction in MGYG000004462_00045.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	32	682	1.8e-134	0.9860869565217392

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	2.42e-85	33	669	5	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
pfam18370	RGI_lyase	6.81e-07	33	118	7	84	Rhamnogalacturonan I lyases beta-sheet domain. This is the beta-sheet domain found in rhamnogalacturonan (RG) lyases, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 carrying this domain, such as YesW (EC:4.2.2.23) and YesX (EC:4.2.2.24), cleave glycoside bonds between rhamnose and galacturonic acid residues in RG-I through a beta-elimination reaction. Other family members carrying this domain are hemagglutinin A, lysine gingipain (Kgp) and Chitinase C (EC:3.2.1.14).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGB28972.1	4.14e-140	11	855	8	832
ACL74801.1	1.73e-84	18	687	31	631
AKC82987.1	2.48e-83	11	787	11	744
AEY65570.1	8.43e-82	18	687	31	631
QJE98692.1	4.08e-78	25	785	23	732

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CAG_A	4.76e-62	34	685	16	589	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2Z8R_A	7.24e-60	34	685	10	582	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
2ZUY_A	4.82e-50	24	681	3	599	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	6.97e-59	34	685	47	619	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	2.32e-49	24	681	3	599	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000336	0.999111	0.000140	0.000155	0.000133	0.000129

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004462_00045.