CAZyme Information: MGYG000004462_00738

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000004462_00738
• CAZy Family: GH28
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1586		179009.2	7.4454

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004462	2469543	MAG	Israel	Asia

• Gene Location: Start: 59387; End: 64147 Strand: +

Full Sequence      

MRHYLKLMLLCLLFPAAAMADGWDTTYKQIEQSIRQPQFADREFNITKYGASTTAKAAVN
QKAINKAIAACSKAGGGRVVVPAGTFNTAAITMLSHVNLVVEKGAVLQFTFEPELYPVVL
TRWEGLDCWNISPCIYAYKQTDVAITGEGTIDGGGTRETWWPWCGAAKYGWKEGMISQRN
GARPRLLKMAEDGVDMNERRFGPTDGLRPQLINFNTCDGILIENVTLLRSPFWVIHPLLS
KNITVRGVHINNDGPNGDGCDPESCDGVLIENCFFNTGDDCIAIKSGRNNDGRLWNKPSE
NIIIRNCKMENGHGGVVIGSEISGGCRNVYAENNVMDSPNLDRVIRIKTNTCRGGVIENI
YARNIKVGQCGESVLKINLDYEHNEICCRGFLPTVRNVNLDNITCEKSKYGVQIIALDTC
TNVYDINVKNCRFNGVAEGNTIKGQTRNVNFDNLYINGGLSLLNKPYKTYSQWMTASEMK
RVPQSYMLDFSNRPKWSYVMGIELEAMLDTYLKYGGDDIKRYCQQYTDTMINAKGDIRGY
RLEDYNLDQIRTGHFVARMYQLYPEAKNRKALETLMKQLQKQPRTEDGVFWHKAIYSYQV
WLDGIFMGLPYYAMTANMLLKDKKAQAIYDDAVDQIKKTYERTLDKKTGLNRHAWDETRE
MFWADKETGLSQHCWGRAQGWYTMALIELLDALPENYSRRGEVIELLKKDLDAIIKWQDK
ATGVWYQVMDSPKREGNYLESTCSSMFTYALLKAYRKGYVGSKYRDAGIKAYRGLINNFI
KVNPDLTISLTGCCKVAGLGPGMSDKVKAATEKYGMKLKENKRRDGSYAYYLSEEVRDND
AKGVGPFIWASLEMEALGYNTDNATATINRQAVVTRNNPVVTEADALASLTVGNGHFATT
VDVTGLQSYPFDYEAGVPLNAMSDWGWHSFKNTKGLTPAETERTMNLGHGRDEVFAVEYK
TDGRNKEATEYFRANPHRLNLGTIGLMLADKDGKQIPLKELKNVRQEQALWDGTIESRFT
ADGTPVEVTTACMSDKDAIFARIKSPLLHEGRAAVTFRFSYPSGKHADDANDWTKPEKHS
SKIVSSDSCSAIIERIIDGTRYYVTLTWEGNASIKETDTPHCFALVSKEDVLSFCAEYTP
KQNRMRPGAFEYDQALKGVMKYWPRFWNSGAIVDFSDCTDPRAKELERRTVLSQYLTAIN
CANTTPPQETGLTYNSWYGRPHLEMTWWHAVDFALWNRPEVVATMLDWYNETAYPVAKKI
AERQGFKGVRWMKMTDPWAGESPSNVGSFLTWQQPHYIYMAEEMYRAKPNGETLKKYAKQ
VEETAEFMADFAKACAPKKGDIKLFGQTAMQESMSKDFSFNHPFEQVYWVYGLNVAQKWR
ERQGLERNAEWDELISRMGSLAEKDGIYTAGEPSQAFDPKAKSAGFDPFNTGGQNTAKQI
SAEDFALKSRSDHPAVLGACGLLPATGQYDEAKMKATLDWVMKNWNWDTTWGWDYGMIAM
CAARLGEPATALEALLINKGKNTYLKSGHNYQEPKRLRLYLPGNGSLLDAVAMMCAGWDG
CKDVLNPGFPKDGTWNVRWEGLNRMQ

Enzyme Prediction     

EC	3.2.1.67

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH105	488	855	1.6e-115	0.9819277108433735
GH28	69	449	2.7e-78	0.9661538461538461

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	4.51e-86	33	423	72	451	Polygalacturonase [Carbohydrate transport and metabolism].
pfam07470	Glyco_hydro_88	2.08e-85	470	855	1	340	Glycosyl Hydrolase Family 88. Unsaturated glucuronyl hydrolase catalyzes the hydrolytic release of unsaturated glucuronic acids from oligosaccharides (EC:3.2.1.-) produced by the reactions of polysaccharide lyases.
COG4225	YesR	5.49e-72	496	857	34	356	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].
pfam00295	Glyco_hydro_28	4.37e-25	123	423	41	296	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
PLN03003	PLN03003	2.98e-11	44	290	24	221	Probable polygalacturonase At3g15720

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADE82894.1	0.0	2	1586	3	1596
QVJ80657.1	0.0	2	1586	3	1596
QQR09854.1	0.0	1	861	1	856
ARE60790.1	0.0	1	861	1	856
ASB36694.1	0.0	1	861	1	856

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3JUR_A	8.86e-98	28	418	8	396	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]
4WU0_A	8.88e-78	496	857	23	361	StructuralAnalysis of C. acetobutylicum ATCC 824 Glycoside Hydrolase From Family 105 [Clostridium acetobutylicum ATCC 824],4WU0_B Structural Analysis of C. acetobutylicum ATCC 824 Glycoside Hydrolase From Family 105 [Clostridium acetobutylicum ATCC 824]
5OLP_A	6.71e-71	32	454	33	441	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]
1NC5_A	5.62e-51	495	857	38	367	Structureof Protein of Unknown Function of YteR from Bacillus Subtilis [Bacillus subtilis],2D8L_A Crystal Structure of Unsaturated Rhamnogalacturonyl Hydrolase in complex with dGlcA-GalNAc [Bacillus subtilis]
2GH4_A	2.00e-50	495	857	28	357	ChainA, Putative glycosyl hydrolase yteR [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O34559	3.08e-50	495	857	38	367	Unsaturated rhamnogalacturonyl hydrolase YteR OS=Bacillus subtilis (strain 168) OX=224308 GN=yteR PE=1 SV=1
A7PZL3	2.85e-35	59	365	77	356	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1
P0A3U6	5.87e-25	607	853	1	227	Protein Atu3128 OS=Agrobacterium fabrum (strain C58 / ATCC 33970) OX=176299 GN=Atu3128 PE=3 SV=1
P0A3U7	5.87e-25	607	853	1	227	24.9 kDa protein in picA locus OS=Rhizobium radiobacter OX=358 PE=2 SV=1
P27644	1.16e-20	216	365	29	181	Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000247	0.999041	0.000177	0.000165	0.000160	0.000140

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004462_00738.