CAZyme Information: MGYG000004476_00912

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; Borkfalkiaceae; UBA1259
• CAZyme ID: MGYG000004476_00912
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
807	MGYG000004476_12|CGC1	92601.31	7.2411

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004476	1651123	MAG	Israel	Asia

• Gene Location: Start: 30936; End: 33359 Strand: -

Full Sequence      

MDKITEKEATSLIKSKLSKYFAVSPKEASREQIYKAVVMCVKDMLLEKRSAFNKKYRARD
GKRVYYLCMEFLLGQSLKNNTYNLNIQKSFDEALKKEFGCSLDDLYEIEPDAGLGNGGLG
RLAACFMDALATQNYPAMGYSIRYEYGLFKQKIVDGWQMELPDIWLPGGEVWLSQRLDKT
YKVKFDGHIKENWTENGLKIEHIDATEVEAVAYDMMISGKDSEAVSILRLWKTQNIHKFD
MKTFSQGDYMRCMREDNEAELISKVLYPDDNNFEGKSLRLKQQYLLVSAALQDIISDHIR
RYGSLDTLPDKAAIHINDTHPALCIPELMRILIDENGYSWEKAWDIVTRTVAYTNHTVMS
EALEKWNEDLIARKLPRIYSILKEINQRFCAEMWNRFYGDWDKIDRMSIFSHSQIKMANL
SVIASHTVNGVSALHSDIIKNSIFKDFYDIYPQKFTNVTNGIAHRRWLCQSNPELSALLN
ECIGDGYVLDASRLSDFKKFASDKGVLDKLNEIKKIKKNQFCKYAKDTEGLIIDPETLFD
VQAKRMHEYKRQLLNALYIISLYNELLENPDAEMVPKTFIFGAKAAPGYYFAKKIIKLIC
CIGEEIKKNPRINKKINVVYMEDYRVSMAEKLMPATEISEQISQAGKEASGTGNMKFMIN
GALTIGTLDGANVEMRKVCGNDNIFIFGLTEDEVDKLWREGYNSTQYYMNNEKLKKVIAA
LNKGFNGQSFSDIANYLLTGSPVADPYMCLADFGAFCDIHDKADSVYKNKTEWAKKSLYN
IASSGIFAADRSIREYSDKIWHLKRID

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	96	804	1.4e-262	0.9955489614243324

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	25	806	33	813	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	13	805	13	750	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	24	805	25	798	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	13	803	1	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	13	803	4	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVQ97458.1	0.0	8	807	9	807
AYF42955.1	0.0	8	807	9	807
QCN93713.1	0.0	8	807	9	807
AYF40115.1	0.0	8	807	9	807
ADU27514.1	0.0	8	807	46	844

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1Z8D_A	3.33e-240	28	801	47	826	ChainA, Glycogen phosphorylase, muscle form [Homo sapiens]
2GJ4_A	4.20e-239	28	801	35	814	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2GM9_A	4.34e-239	28	801	35	814	Structureof rabbit muscle glycogen phosphorylase in complex with thienopyrrole [Oryctolagus cuniculus],5MCB_A Glycogen phosphorylase in complex with chlorogenic acid. [Oryctolagus cuniculus],7ONF_A Chain A, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
2FFR_A	4.34e-239	28	801	35	814	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
7O8E_A	5.11e-239	28	801	40	819	ChainA, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39123	7.51e-249	26	804	25	795	Glycogen phosphorylase OS=Bacillus subtilis (strain 168) OX=224308 GN=glgP PE=2 SV=1
Q9XTL9	4.12e-244	34	801	53	826	Glycogen phosphorylase OS=Drosophila melanogaster OX=7227 GN=GlyP PE=2 SV=2
P0AC86	2.33e-241	25	803	33	810	Glycogen phosphorylase OS=Escherichia coli (strain K12) OX=83333 GN=glgP PE=3 SV=1
P0AC87	2.33e-241	25	803	33	810	Glycogen phosphorylase OS=Shigella flexneri OX=623 GN=glgP PE=3 SV=1
Q3B7M9	1.17e-240	28	801	47	826	Glycogen phosphorylase, brain form OS=Bos taurus OX=9913 GN=PYGB PE=2 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000034	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004476_00912.