CAZyme Information: MGYG000004477_00472

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-508; CAG-273
• CAZyme ID: MGYG000004477_00472
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
418	MGYG000004477_2|CGC2	48397.88	8.9703

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004477	1162770	MAG	Israel	Asia

• Gene Location: Start: 106764; End: 108020 Strand: -

Full Sequence      

MEYLYIIQQALVWIITIFWLYQLAISVCSLVKLKDKPMLVNKNHRFMAIIPAHNEENVVT
ELIESLKKQNYPKELLDIYVIADNCTDRTAEVARNAGAIVYERFDPSKKTKGYALNWFLR
QKIDENAPYDAFCIFDADNIVDKDFIKNMNKKLCQGEDVVQGYRDIKNPTDNWITAGYAI
FYWTMNRFYHLARYNVGLSPLINGTGFMVRFDVVKPEGWVTKTLTEDIEFSLKRIIKGKK
LGWAVDAVVYDEQPLGFKQSWKQRTRWTVGHMQCIKEYTKPLALAVKEHKTVMNFDGFLY
IIGSIPMFVLTLVLLAINVIMYAGNGMTTGDLIVNMLRYVIPTFLLPIATAVLIMLLDKR
PIKPMINGLLCYPLFLGSWIAINFKCLFKRDTTWEKIDHVRKIAIHEVSNVETEKVQN

Enzyme Prediction     

No EC number prediction in MGYG000004477_00472.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	45	270	8.9e-27	0.991304347826087

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06438	EpsO_like	4.84e-89	49	230	2	183	EpsO protein participates in the methanolan synthesis. The Methylobacillus sp EpsO protein is predicted to participate in the methanolan synthesis. Methanolan is an exopolysaccharide (EPS), composed of glucose, mannose and galactose. A 21 genes cluster was predicted to participate in the methanolan synthesis. Gene disruption analysis revealed that EpsO is one of the glycosyltransferase enzymes involved in the synthesis of repeating sugar units onto the lipid carrier.
cd06423	CESA_like	3.02e-50	49	227	2	180	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.
COG1215	BcsA	8.50e-45	10	387	16	411	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
pfam13641	Glyco_tranf_2_3	9.28e-24	45	267	3	227	Glycosyltransferase like family 2. Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis.
cd06421	CESA_CelA_like	9.28e-21	45	273	2	232	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWT55360.1	1.54e-112	20	409	23	414
BCI60981.1	5.80e-111	6	408	10	413
CDZ24461.1	2.32e-107	16	410	19	411
BBB93061.1	5.95e-98	14	408	15	407
AYF39240.1	2.17e-97	18	411	22	415

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5TZE_C	4.95e-07	45	148	2	103	Crystalstructure of S. aureus TarS in complex with UDP-GlcNAc [Staphylococcus aureus],5TZE_E Crystal structure of S. aureus TarS in complex with UDP-GlcNAc [Staphylococcus aureus],5TZI_C Crystal structure of S. aureus TarS 1-349 [Staphylococcus aureus],5TZJ_A Crystal structure of S. aureus TarS 1-349 in complex with UDP-GlcNAc [Staphylococcus aureus],5TZJ_C Crystal structure of S. aureus TarS 1-349 in complex with UDP-GlcNAc [Staphylococcus aureus],5TZK_C Crystal structure of S. aureus TarS 1-349 in complex with UDP [Staphylococcus aureus]
5TZ8_A	6.81e-07	45	148	2	103	Crystalstructure of S. aureus TarS [Staphylococcus aureus],5TZ8_B Crystal structure of S. aureus TarS [Staphylococcus aureus],5TZ8_C Crystal structure of S. aureus TarS [Staphylococcus aureus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P96587	2.05e-18	49	300	54	306	Uncharacterized glycosyltransferase YdaM OS=Bacillus subtilis (strain 168) OX=224308 GN=ydaM PE=3 SV=1
P74165	9.74e-12	49	306	113	371	Beta-monoglucosyldiacylglycerol synthase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1377 PE=1 SV=1
Q47536	4.13e-08	48	282	34	266	Uncharacterized glycosyltransferase YaiP OS=Escherichia coli (strain K12) OX=83333 GN=yaiP PE=3 SV=2
Q6G608	1.00e-07	49	270	52	270	Poly-beta-1,6-N-acetyl-D-glucosamine synthase OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=icaA PE=3 SV=1
Q8NUI7	1.00e-07	49	270	52	270	Poly-beta-1,6-N-acetyl-D-glucosamine synthase OS=Staphylococcus aureus (strain MW2) OX=196620 GN=icaA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000026	0.000014	0.000001	0.000000	0.000000	0.000001

TMHMM  Annotations     

start	end
10	32
298	320
335	357
364	382