CAZyme Information: MGYG000004519_00812

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; TWA4
• CAZyme ID: MGYG000004519_00812
• CAZy Family: GH36
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
545	MGYG000004519_34|CGC1	62484.03	4.8888

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004519	2064379	MAG	Israel	Asia

• Gene Location: Start: 15027; End: 16664 Strand: +

Full Sequence      

MNTTCMKLGVPNYITIATQTETFGCRPLAEQITVDDMVLEMKTVDSTLAISLTATKTPVK
YLKFRWNKEWPAGVRFLGDAWERGYGDLEWRGMSANRFMPWYFCATKEDTHLCYGVKVRP
SALCFWQVDSEGITLFMDVRCGGNGVKLNGRTLEVAKVLNMSATECTSFEAISEFCGKMC
TDPILPPYPVYGSNNWYYAYGKSSEEEILSDCDYILGLTKDVTNKPYMVIDDCWQEHHRL
DEYNGGPWTKGNSKFPDMKGLADKLAEKGVRPGIWVRLLLNEDEKIPNEWRLPHNGCLDP
SHPQALEYIKADVKRICDWGYTLIKHDFSTFDLFGRWGFEMNPLMTAEADWHFYDESLTS
AEIVKILYKGILEACNETNTLVLGCNTIGHLGAGYMHINRTGDDTSGVDWERTRRMGINT
LAFRLPQHRKFYEVDADCVGIDSGIDWYFNKQWADLLAESGTSLFVSARPGLLNDEEFKE
LQDIMKVASTQDHHLIPMDWEDTDCPMKWEDKENKIVRNYHWYEDCGVKFSTKTVRALPY
LSMAE

Enzyme Prediction     

No EC number prediction in MGYG000004519_00812.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14791	GH36	1.18e-42	188	486	1	295	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
cd14792	GH27	5.97e-12	195	328	6	124	glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
COG3345	GalA	2.44e-09	187	439	291	565	Alpha-galactosidase [Carbohydrate transport and metabolism].
cd14790	GH_D	3.40e-06	196	328	8	107	Glycoside hydrolases, clan D. This group of glycosyl hydrolase families is comprised of glycosyl hydrolase family 31 (GH31), family 36 (GH36), and family 27 (GH27). These structurally and mechanistically related protein families are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively. They have a wide range of functions including alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase, alpha-N-acetylgalactosaminidase, stachyose synthase, raffinose synthase, and alpha-1,4-glucan lyase.
cd06592	GH31_NET37	4.83e-05	188	318	2	140	glucosidase NET37. NET37 (also known as KIAA1161) is a human lamina-associated nuclear envelope transmembrane protein. A member of the glycosyl hydrolase family 31 (GH31) , it has been shown to be required for myogenic differentiation of C2C12 cells. Related proteins are found in eukaryotes and prokaryotes. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SNU96820.1	2.47e-306	11	543	11	543
QIB59833.1	7.91e-299	6	545	6	548
BDA09953.1	1.02e-294	6	545	6	548
CBL19648.1	1.26e-258	7	527	27	544
QCU01578.1	2.94e-257	7	527	27	544

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5F7C_A	2.78e-06	183	328	304	460	Crystalstructure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5F7C_B Crystal structure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5F7C_C Crystal structure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5F7C_D Crystal structure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004519_00812.