CAZyme Information: MGYG000004539_00421

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-508; CAG-273
• CAZyme ID: MGYG000004539_00421
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2717		313618.79	7.3429

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004539	1223898	MAG	France	Europe

• Gene Location: Start: 151575; End: 159728 Strand: -

Full Sequence      

MKDYKVLNIKGALLDKVQLENYLEKIASDNVLQAKSNKETYPIPRLKENYEIITQIYELL
TEHIKLKIPIHPAGEWILDNYYIIEETVKSIVKDVTLKKYINFPGIATGPYAGFARIYEL
AGEIVAYTDCKIDSKTLPELLKAYQAKKNLNMEEIWNIGPFIQIALIEKVREVCEKIYSS
QMQKYKVENIVERLVEEKEKEKCKFKNIHEYRLKIIGYEEMKYPFIEYMSYKLKKLGRKG
YPFLKVLEDEVNKMGLDISDVIKKEHFDIAVKKVTVGNSITSIKAINRMNFVNIFEEING
VEEILKKDPTETYSKMDYKTRIYYRNAIKKIAKKTGISEIYIAQKALELANKEFNKEDRD
VLNLKKSHIGYYLISDGRNSLIEEVTNKKVGAISNNDKAKIYIYGIMAISVIIVVLLACN
IEKKVNNLLATIIVSILLFVPVQVIVQQIVQYILSKFVSPKIIPKLDFNSGIPKEYSTFI
VIPTILNSTQKVDELVKKLEVYYLANKSENLYLAILGDCTPSSQKVEEIDEKIIREGLKQ
IEKLNKKYDNNKFPKFHFLYRERIWNDGEACYLGWERKRGILNQFNEFIKKGDNGNFKVN
TIEQAKEKTKLPNIKYVITLDSDTNLVLNSGLELVGAMAHILNIPILNKSNTAVESGHAL
IQPRVGIDLLISNKTWFTKIYAGNGGTDSYSNAISDTYQDNFEEGIFTGKGIYDLDIFYE
IFKNTIPDNTVLSHDLLEGNYLKCGLASDIELMDGYPTTYQTYKMRLSRWIRGDWQIIGW
LKKRVLDRHKEKRNNPLNKLSKYKILDNLFRSVLEINSVLLILATIIIWMWKRVNFVSIL
VIAVIAVIIPTIIDIVNKVIFYKEGEKKQKTFVKNIGNFRASVLRGWLAIALLPDRAYMS
LCAIVRTIYRMFRSKKNLLEWTTAEEAENSKKNSLQSYYSSMGPNIALALIILAISPYIH
NVFINGLIIVLSLIWLTAPLIMWYISKDIEEGLQIDKLNKEDKEFIIDIAQKTWNFFKEN
LTEKSNYLPPDNFQEDRVPKIVYRTSPTNIGLAMLAVISSYDLKFESLDNTLKLLDKMIN
TIAELPKWNGHLYNWYDIKNLKPLEPKYISSVDSGNFIGYLYVVKEFLEEVKEVYENVDS
MLEIISNVINNTDFSKLYNKENMLFSIGFNVEENSLTDSYYDLLASEARQTSLVAIAKKD
VEVKHWENLSRTLTKFKNYKGLLSWSGTSFEYLMPNVNIPKYPGSLLDESCKFMVMSQQD
YAKMLNIPWGFSEAAFNLKDLSNNYQYKAFGIPWLGLKRGLSEEFVVAPYGSAMALYDEP
VNVIDNLKELQKSNMYNKYGFYESIDYTVARLKKGEEYANVKTYMAHHQGLILLSINNLI
NNNIFPERFMKNPEIKSVDILLQEKMPESLIITKEKKEKVERVVNFDYESYTQREISKIN
NNLKEINVISNNNYTIVMDEKGNGYSKYKDILINRYKKTDDIEQGIFFYFKNIRSKRIWT
TSYMTYLNKPDKYTIYFAPDSNKIVRQDGNIETVLKTTISANDPVELRKVKLTNTGLTEE
IIEVTSALEPMLSKREQDYAHKVFNNLFLSYEWIEKPEILLVKRNARGENEEEVYLALNL
YTENETIGEIEFETDKEKFLSRNNLGLPKSVENSTPFSKKTGISTETIAAMKRMVNIMPE
QSVEFNLIIAVGKTREEALGRVVEFKNEEKIKRSFNLARAKVETENNYLGIKGKDVELYQ
KMLRYLVFINPLKSILYKERTNEHALVEDLWKYGISGDIPILLVKIKDANDIEIVKETIK
AYDYFRIKNIEIDLVIINEEKNSYNNYVKEGVQNVIFNQGLGFMQNVKGGIFLLNGLSKK
DKENIEYRANLLINAGMGSILRQIKDLEEEYLENTKEIGDESNLRGIQEDNSYTNNVTTE
ELKYYNDYGGFSEDGTEYKIKVSKDTKLPTVWSHVLANKNFGSVVTESMGGYTWFKNSRL
NRITAWNNNPVTDVPSEVIYLEDMETKKVWSMGLNPVPDDSPYYITYGFGYAKYAHISDG
LKQSVDVFVPMNENVKVQVLTLENTQLKKKEVKIIYYLKPVLDEDEIKSNGFNKLEYNNK
FNTIFMQNITKPVSTNIMWVSSSENILSYTGCKQSFFGKGNLNAPDGVRKIELDNKNSLW
QDGIIAIEMKVEIEALSKKEIVLMVGAGESMLECQDTAYKYSNLNNVRTEFMNVKKYWNS
LVNHLQVKTPVESMNIMLNGWLLYQTICSRIWARSGYYQSGGAFGFRDQLQDAMATKYVD
IDIMKNQIIKHSKHQFLEGDVEHWWHDENGRGIRTRFSDDLLWLVFVVLDYVYFTGNKDI
LEMETPYLYGAPLEEGVSERYDKYSNSDVVEDIYNHCIRAIERSLNFGENGLPKIGSGDW
NDGLSKVGIEGKGESVWLGFFMYIILIKILPICRYRGEEERAKRYEEIAEKLRKALNTNA
WDGRWYKRAFMDSGDALGSIENEECKIDSISQSWATISNAGDNDKKYIALESLENHLVDR
ENGIIKLLDPPFNKGTLDPGYIKSYLPGIRENGGQYTHGAIWVIIAEAMLGFGDKATEFF
RMINPIEHSRTKDSANKYKVEPYVMAADVYGKESLSGRGGWTWYTGSSSWMYEAGIKYIL
GLNIVRDFLRIKPCIPKEWKEYSIKYRYGSSIYNITVKNESGKNTGVEKFILNGEEIPDK
QVKLSRAGGVYHIDIIM

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1632	2709	0	0.9922779922779923
GT84	1179	1393	2.4e-88	0.9953488372093023

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	0.0	1631	2717	1	1053	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	1.06e-134	2217	2642	1	424	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
cd11753	GH94N_ChvB_NdvB_2_like	4.12e-73	1387	1717	3	336	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
cd11756	GH94N_ChvB_NdvB_1_like	7.53e-72	1921	2202	1	284	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	3.11e-70	1949	2186	1	247	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUG57227.1	0.0	6	2717	31	2909
AEV69563.1	0.0	7	2717	32	2919
QCX34529.1	0.0	7	2717	29	2710
ADU74104.1	0.0	7	2717	36	2919
ANV75789.1	0.0	7	2717	36	2919

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3QDE_A	1.86e-95	1928	2717	3	810	ChainA, Cellobiose phosphorylase [Acetivibrio thermocellus],3QDE_B Chain B, Cellobiose phosphorylase [Acetivibrio thermocellus]
1V7V_A	1.48e-81	1928	2697	3	781	Crystalstructure of Vibrio proteolyticus chitobiose phosphorylase [Vibrio proteolyticus],1V7W_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc [Vibrio proteolyticus],1V7X_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate [Vibrio proteolyticus]
3S4C_A	1.39e-64	1928	2717	3	822	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]
3RRS_A	3.36e-64	1928	2717	3	822	Crystalstructure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RRS_B Crystal structure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RSY_A Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3RSY_B Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3S4A_A Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4A_B Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4B_A Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda],3S4B_B Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda]
2CQS_A	4.50e-64	1928	2715	23	840	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	0.0	45	2681	61	2809	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
B9K7M6	4.83e-91	1928	2717	3	812	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1
Q9F8X1	2.21e-87	1928	2717	3	799	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1
Q76IQ9	7.20e-81	1928	2697	3	781	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000061	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
401	418
425	447
809	831
836	853
883	905
963	985