CAZyme Information: MGYG000004558_02270

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter
• CAZyme ID: MGYG000004558_02270
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
641	MGYG000004558_14|CGC2	74702.68	6.6743

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004558	3542236	MAG	France	Europe

• Gene Location: Start: 48528; End: 50453 Strand: +

Full Sequence      

MKKGKSTKKKLEPFEIGELDQYLFGQGNHYEIYEKMGAHKVVYKGREGVYFAVWAPNARR
VAVVGDFNGWDFEANYMERQEPVGIYTCFVPGVKEGDLYKFCIETQQGKRIFKADPFANY
AELRPGTASRVTDISHLKWTDSAWMKRRETWDHRENPMSIYEVHLGSWMRHPGRDDEGFY
TYREFADAIVKYVKDMGYTHVELMGMAEHPFDGSWGYQVTGYYAPTSRYGTPEDFAYMID
HLHRNNIGVILDWVPAHFPRDAHGLADFDGTPTYEYADPRKGEHPDWGTKIFDYGKPEVK
NFLIANALFWIEHYHVDGLRVDAVASMLYLDYGKQDGQWVANKYGGNENLEAIEFFKHLN
SVVLGRNKGAVMIAEESTAWPKVTGAPEDDGLGFSLKWNMGWMHDFTEYMKLDPYFRKNA
HHMMTFASSYAYSENYILVLSHDEVVHLKCSMLNKMPGLGFDKYANLKVGYAFMTGHPGK
KLLFMGQEFAQLREWSEERELDWFLLAEPEHQAIQNWYRDLLHLYKKNKALYELDSYPEG
FEWINADDIFRSIYSFERHSRDNKKNLLFVCNFTPMERPDYRVGVTRRKQLKLVLNSDEK
KYGGKGVERPLIYKPVKQECDGKKYSIAYPLPAYGVAVFEF

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	192	491	1.8e-150	0.9933554817275747
CBM48	35	119	2e-16	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	18	641	9	627	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	16	639	603	1220	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	16	640	6	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14706	PRK14706	0.0	36	641	27	621	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	16	640	100	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHB24149.1	0.0	1	641	1	641
QEI31650.1	0.0	1	641	1	641
QRT30382.1	0.0	1	641	1	641
CBL27570.1	0.0	1	641	1	641
QEK19514.1	0.0	9	641	4	636

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	4.96e-225	1	640	109	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	4.96e-225	1	640	109	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	7.03e-225	1	640	109	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	9.95e-225	1	640	109	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	1.41e-224	1	640	109	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	5.87e-307	16	641	7	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q7NL20	4.00e-245	16	640	103	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
Q8DLB8	9.03e-237	15	640	105	749	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q2JK68	2.32e-236	16	607	117	719	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=glgB PE=3 SV=1
Q1AZ86	8.78e-234	13	632	96	710	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004558_02270.