CAZyme Information: MGYG000004559_01525

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; CAG-831
• CAZyme ID: MGYG000004559_01525
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1170		130189.53	4.6564

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004559	2216405	MAG	France	Europe

• Gene Location: Start: 5267; End: 8779 Strand: -

Full Sequence      

MTSIGFAAAALLAAVTASAGTASARVTNDQDGELPYWQDVQTVEVNREYPRAAFMSYPDS
ATAAACRYENSPRYLLLNGVWKFYFVDAYGDLPADITDPATDASGWHDINVPGNWEVQGF
GTAIYTNHGYEFKPRNPTPPLLPEDNPVGVYRREFEVPASWDGSEIFLNIDGAKSGVYVY
LNGREVGYSEDSKTSAEFAVSDYLVPGTNTLVLKIFRWSTGSYLECQDFWRISGIERDVY
LWCQPREAALKDFTVISTLDDNYEDGLFSLSAVLKNTSDKATAATLRATLAGPDGAVVWS
DCGSTEIEAGGTADVWLETELEDVLPWSAEHPDLYRLTMTVCADGREVETVPYHVGFRRF
EIRPSGGVSPEGRPYVLFYVNGQPVKLKGVNTHEHNPLTGHYVTEDLIRKDLELMKQNNI
NSVRLSHYPQGRRFYELCDQYGLYVYDEANIESHGMYYNLSKGGTLGNNPEWLAAHMDRT
VNMYERNKNYPCITVWSLGNEAGNGYNFYQTYLWLKDRETGGMNRPVCYERAQWEWNSDM
YVPQYPDAAWLRKIGRAGSDRPVVPSEYSHAMGNSSGSLSLQWDEIYRYPNLQGGYIWDW
VDQGIWQDRDGGFWAYGGDFGVNAPSDGNFLCNGIVNPDRNPHPAMAEVKHTYQNAAFRP
AGYTEDPTAERTLSTGRLAFASVPSDGKAVVEVINRFYFTPLDGYDFEYELTADGRVAAS
GTFAVKAAPQDTVQVALDLPAMDSKGVEYFLNLYMKQRNAVAGIPAGHVLAWDQIGFPVS
GLSQASATPSAGSRAAADRPSVNASARAKGPALSIDSASSDGVVRISSSSVEWVFDRSAA
AVTSYKVRGVEYFADGYGLRPNFWRGPNDNDYGNGAPKRLQIWKISSNDLRVGSVSAVMD
GSDAVMTVEYLLAAGNRYIAEYTVHPDGVVDAAFHFTPALMPEVENEASEATLTATYTPG
QESRVGLKARPEVPRIGVRFRLPVQMHDVTWYGRGPGENYIDRSRGSMVGLYTSSAEEMY
YPYVRPQENGHRSDTRWAAFCGGGNGLMVVAKATFGFNALRNPVEDFDSEEAVEHDYQWY
NFTPEQIASRDTAAVRNVLRRMHHVNDIVPQDYVEVCIDMRQQGVAGYDSWGDRPEPQYR
LPADRDYTWGFTLVPVKNARDAARKAILDY

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	64	1009	6.4e-199	0.973404255319149

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09525	lacZ	0.0	37	1153	17	1024	beta-galactosidase.
PRK10340	ebgA	0.0	37	1159	4	1003	cryptic beta-D-galactosidase subunit alpha; Reviewed
COG3250	LacZ	8.48e-132	77	1003	15	800	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
smart01038	Bgal_small_N	2.68e-91	827	1153	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.
pfam02836	Glyco_hydro_2_C	2.29e-88	377	658	7	302	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABR42015.1	0.0	35	1157	33	1112
QRO17774.1	0.0	35	1157	35	1114
QCY58502.1	0.0	35	1157	14	1093
QJE28487.1	0.0	35	1157	33	1112
QUT53259.1	0.0	35	1157	33	1112

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6SD0_A	2.44e-207	34	1153	1	980	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
6S6Z_A	6.59e-207	35	1153	1	979	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
3DEC_A	2.15e-196	33	1157	3	998	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3BGA_A	2.18e-191	34	1157	7	1002	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	1.24e-139	37	1036	16	942	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56307	1.33e-206	34	1153	1	980	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
O52847	1.02e-183	35	1156	18	1034	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q6LL68	1.30e-154	37	1158	12	1030	Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1
A5F5U6	2.18e-154	37	1141	13	1005	Beta-galactosidase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=lacZ PE=3 SV=2
A0KQH4	6.37e-153	37	1153	11	1024	Beta-galactosidase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000350	0.998976	0.000155	0.000191	0.000153	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004559_01525.