CAZyme Information: MGYG000004578_00485

Basic Information

• Species: UMGS1795 sp900553555
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-552; UMGS1795; UMGS1795 sp900553555
• CAZyme ID: MGYG000004578_00485
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
278	MGYG000004578_4|CGC2	32596.05	9.8148

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004578	1540237	MAG	France	Europe

• Gene Location: Start: 87635; End: 88471 Strand: +

Full Sequence      

MINLLFAGNGKVFDGILTCLLSIFMRSELDRPVTAYILTMDVSRIKAEYTPIDDRRMQVL
SDVVAKFNPKNRVVKIDVTELYEREFAYCPNENAYCSPYTLLRLFADVVDIPAEKLLYLD
ADLMFNRDLRLLYDTDVSGVEYAAARDRYGKMLVSPNYINGGVMLFNMQKVRQTGLLKKA
RRLIKTKKLPFADQSAIIRSTEKKKVLPQRFNNQKKLRKDTVVLHFSKRLFYLPYPHTDN
IKQWQVERVHKVYGYHCFDDIYEQYFIALNSLKQEVKK

Enzyme Prediction     

No EC number prediction in MGYG000004578_00485.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	3	227	2e-28	0.8404669260700389

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	1.41e-28	2	226	1	239	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
pfam01501	Glyco_transf_8	8.37e-12	3	227	1	242	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
cd00505	Glyco_transf_8	1.16e-10	76	227	62	238	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
COG1442	RfaJ	6.89e-10	2	226	3	237	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQA01162.1	2.68e-116	1	278	1	278
QNL97925.1	1.00e-112	1	277	1	277
AEE15925.1	3.95e-68	1	274	1	272
QAT42778.1	4.49e-58	3	265	2	266
ANK66321.1	2.25e-54	3	273	2	274

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004578_00485.