CAZyme Information: MGYG000004587_02199

Basic Information

• Species: UBA1409 sp900553675
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA1409; UBA1409 sp900553675
• CAZyme ID: MGYG000004587_02199
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
951	MGYG000004587_31|CGC1	107740.63	4.8774

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004587	2560840	MAG	France	Europe

• Gene Location: Start: 9234; End: 12089 Strand: +

Full Sequence      

MNKIILNKQTRFIHELQAYSGAKIAARNVMHDMELIFGETVKDYRSGEKVDSAIIYGTVG
SSPILDKLDAEGKIDVGGIRGKWEAYSIAVVAEPIDGVSTAVVVAGSDKRGTIYGLYHLS
ELMGVSPLVNWNHVYPEKRDELVFTDEINMVSKTPSVKYRGFFINDEWPAFGNWANTHFG
GINAKCYERVFELLLRLKGNYLWPAMWGSDFSLDGPDLASAELADEMGVVMSTSHHEPCC
RSGNEYGKVRGKDSEYGDAWSFLANEEGITAFWRDGLIRNSRFENVITMGMRGENDTAIL
GKDATLADNIELLRNVLRTQNRLIKENVNHDLNEVPRQMVLFTEVEEFFYGGNGVDGLMN
DPELDGVTLMLSDNNHGSTRTLPSEAMRNQNHNGGYGMYYHMDMHGGPHSYQWIGSTYLP
KVWEQMTMAYEYGVREIWVTNVGDIGTQEFGLSYFLDLAYDIEKWGGEDAGITNTYTKEW
VKRTFGYAFDNADLAKIYEAVIGYTMLLSRRKHEKINEWTYHPVNFGEADEVLSVSERLL
EICDELKAKCPKNILGAYIALIHYPVCGTANLMKMWVYAAKNRLYASQNRLTANTLADKI
RETIAADYALTAEYHTVDGGYYYGFGLSEHIGFTTWDDANNQYPIMRYVHPANGKRMIIS
KVDDERYVTGLATFDRRLVWTDALRQDICDIEFDITCAGASPMGFRIETDCDWLSFSETE
GEIELVKRVTLHIDKSKFTGKQTGSFCIFGVGFHARAQIFVEAENRTDIPENVFIEDDGY
ICMKAVNYQERHDTENGGFRKLIPCTREGSAIKAFPVTTDFMKVADKPYVAYRFEADADG
EYNIRFWLEASTPVVYEREQFICFSLNGGEMLIVNTVKEVDKQFFQSAQWAQEATDHVKI
TDSVITCRNGVNELRFYAASPAIVLEKIAVYPKGSRLKDSYMGPQESFIRV

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	9	651	1.5e-196	0.8393113342898135

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	1.33e-163	153	514	1	333	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	4.16e-33	773	946	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	0.001	13	119	24	120	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL32929.1	0.0	3	950	38	985
AOZ95221.1	0.0	1	949	1	945
AET60998.1	1.60e-309	12	922	21	931
BAM48432.1	3.71e-303	12	948	20	962
AZS17889.1	1.16e-299	12	948	27	969

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6NPS_A	2.55e-305	12	948	22	964	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
4ZMH_A	1.32e-134	19	947	32	933	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
4C90_A	3.16e-107	21	774	64	788	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	6.39e-103	21	638	37	637	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	2.33e-101	21	638	36	636	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004587_02199.