CAZyme Information: MGYG000004611_00421

Basic Information

• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Acidaminococcus
• CAZyme ID: MGYG000004611_00421
• CAZy Family: GH18
• CAZyme Description: Putative sporulation-specific glycosylase YdhD

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
365	MGYG000004611_8|CGC1	41022.84	6.8322

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004611	1855749	MAG	France	Europe

• Gene Location: Start: 1532; End: 2629 Strand: +

Full Sequence      

MKAKWLILCGLLLSLTGCALGAAPSKVGTETAAERTVERENYTPRKVNGTVHLVWDWQPE
DGSVSQLLEQEKLPGINVVSPKWFEIADENGRIANKNGDIAYVEKAHAKGYIVWPLITNG
FNPDRTRKLLHNPTARQQVVQQLLSLCKTMQLDGINLDFENIHPEDRERLTDFVGEIAEA
LHTNNLTVSIDVTIPSSNGNWSRCYDRGALSAKTDYVMLMAYDEHYRKSKIAGSVASLPW
VEAGVQATLAEKVPKEKLVLGMPLYMRDWTIDKSGQVSAKTLSMSGAEQLLKEKTLVPQW
LPDKGQYYFSYTDEKNQQHQVWQEESRSLLLKAALVSRYDLAGSCYWRKGLETPDVWSKL
AAVMP

Enzyme Prediction     

No EC number prediction in MGYG000004611_00421.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	101	349	1.5e-42	0.7702702702702703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02874	GH18_CFLE_spore_hydrolase	1.02e-98	57	360	8	313	Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
COG3858	YaaH	3.20e-63	66	360	118	418	Spore germination protein YaaH [Cell cycle control, cell division, chromosome partitioning].
cd06549	GH18_trifunctional	3.54e-34	60	357	10	298	GH18 domain of an uncharacterized family of bacterial proteins, which share a common three-domain architecture: an N-terminal glycosyl hydrolase family 18 (GH18) domain, a glycosyl transferase family 2 domain, and a C-terminal polysaccharide deacetylase domain.
cd02875	GH18_chitobiase	1.29e-31	105	360	72	358	Chitobiase (also known as di-N-acetylchitobiase) is a lysosomal glycosidase that hydrolyzes the reducing-end N-acetylglucosamine from the chitobiose core of oligosaccharides during the ordered degradation of asparagine-linked glycoproteins in eukaryotes. Chitobiase can only do so if the asparagine that joins the oligosaccharide to protein is previously removed by a glycosylasparaginase. Chitobiase is therefore the final step in the lysosomal degradation of the protein/carbohydrate linkage component of asparagine-linked glycoproteins. The catalytic domain of chitobiase is an eight-stranded alpha/beta barrel fold similar to that of other family 18 glycosyl hydrolases such as hevamine and chitotriosidase.
smart00636	Glyco_18	8.72e-27	105	347	61	329	Glyco_18 domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADB47041.1	2.02e-140	1	360	1	357
QIB60827.1	1.25e-100	45	357	32	339
BDA10778.1	2.01e-99	45	357	32	339
BBB92123.1	1.93e-98	27	360	142	473
SNU93874.1	2.70e-97	43	357	31	336

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CZ8_A	4.00e-35	90	348	46	307	ChainA, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168],3CZ8_B Chain B, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168]
4S3K_A	7.14e-24	76	357	135	420	ChainA, Spore germination protein YaaH [Priestia megaterium QM B1551]
4WIW_A	6.39e-23	76	350	34	330	ChainA, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_B Chain B, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_C Chain C, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_D Chain D, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_E Chain E, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_F Chain F, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2]
5JH8_A	3.73e-22	134	358	87	308	Crystalstructure of chitinase from Chromobacterium violaceum ATCC 12472 [Chromobacterium violaceum ATCC 12472]
4S3J_A	1.30e-21	117	360	176	421	Crystalstructure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_B Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_C Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O32258	4.86e-37	88	360	63	339	Uncharacterized glycosylase YvbX OS=Bacillus subtilis (strain 168) OX=224308 GN=yvbX PE=3 SV=1
O05495	2.83e-35	90	357	138	409	Putative sporulation-specific glycosylase YdhD OS=Bacillus subtilis (strain 168) OX=224308 GN=ydhD PE=1 SV=2
P37531	1.10e-26	112	361	169	420	Cortical fragment-lytic enzyme OS=Bacillus subtilis (strain 168) OX=224308 GN=sleL PE=1 SV=2
O31682	3.46e-26	76	269	26	212	Putative glycosylase YkvQ OS=Bacillus subtilis (strain 168) OX=224308 GN=ykvQ PE=3 SV=1
P0DPJ9	2.05e-21	117	360	175	420	Cortical fragment-lytic enzyme OS=Bacillus anthracis OX=1392 GN=sleL PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000198	0.076438	0.923213	0.000062	0.000052	0.000040

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004611_00421.