CAZyme Information: MGYG000004612_02167

Basic Information

• Species: UMGS363 sp900768245
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UMGS363; UMGS363 sp900768245
• CAZyme ID: MGYG000004612_02167
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1118		124508.59	4.6122

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004612	2741718	MAG	France	Europe

• Gene Location: Start: 529; End: 3885 Strand: +

Full Sequence      

MSDNRTILFNDNWFFKLLPIDSGYDTAVAQDISEPVEIPHDWLIFNTNDLYASGEGWYKK
VFTIENKGDLIYSIDFDGVYTDSSVYVNGEKAGDWHYGYSSFSFDITSLLKDGENVILVQ
VRHKAPNSRWYSGAGIYRNVYLTKVEPIHIARDGVYISADGKSGEIKVSAEVIGPYDSLR
HTVIDADGNTAAVSDNGLIKVDNYKLWSLENPYLYTLKTEVISNGNVVDTVTNIFGFRWI
KFDANEGFFLNGEYMKLKGVCLHHDLGALGSAVNYRATERQVLLMKEMGVNAIRTSHNMP
SREFLEICDRVGMLVDCESFDMWEMPKTEFDNARFFKDTAHADVLSWVRRDRNHPSVIMW
SIGNEIYDTHASEHGYEIAEMLRNYVLESDPNGNALPTIGSNFIEWENSQKIGKMLGISG
YNYTEKCYDDHHKKYPDTVIYGSETSSAVRSRGIYHFPENVPLLSHDDHQCSSLANSCVG
WGRPAEDAWIQDRNRKFCAGQFIWTGIDYIGEPTPYSTKNSYFGAVDTAGIPKDIFYFYQ
SVWTDVKSNPMVHLLPYWDFNEGQLIDVIAYTNAPKAALFVNGKSYGVQEIDHKNGSILH
GQWLVPYEKGEIRVRATDENGTVIAEDVQKSFGDPAAVKLSADRTEINADGKDLVFVEIS
TADENGVPVANARNRISVEVSGAGRLLGLDNGDSTDYEQYKTDCRKLFSGKLMAVIGSIG
TAGDIVVKASSLGLAPAEITVKAVGAGIPNIKLVEQIRHTEPTDDVPIRKIEASSVGGGA
LDENRTSLEVKLKIFPENATYNDITCKIVQKNSVETNIAEAELRDGSIFVAAKGDGECIL
RIYANNGSEYPQVISDVPLTISGLGSTTRDPYTFVSACYYDSSSIPVNILEKGALGGFTE
PCRISYANIDFGKVGTDELRLYIGNCMNRDIPVEIFDGECEDEKQLIMTAMFPHNNGWDY
ARPYDFKLPSKLKGIHTLSFKIADKCIFGGFEFTAEEKAFAELSPADNDALYGDDYAVNG
GSIEKIGNNVVIGFNDMDFGAGTEKIVICGRTPNEVNTIQLRYTENGTSKTQLLEFACAD
EYTCREFALETISGMTDISFVFLPGSNFDFRSFRFIRK

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	548	3.2e-114	0.6170212765957447

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.29e-74	9	559	30	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.15e-45	48	365	52	417	beta-D-glucuronidase; Provisional
PRK10340	ebgA	4.15e-43	42	391	94	468	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam18565	Glyco_hydro2_C5	8.07e-41	639	740	2	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK09525	lacZ	1.68e-28	56	375	122	466	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCN28859.1	0.0	1	1117	1	1155
BCJ94036.1	0.0	8	1115	7	1145
BCJ98579.1	0.0	8	1115	4	1135
CBL35301.1	0.0	5	1117	4	1148
QKS44447.1	0.0	4	1115	2	1156

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	1.59e-211	5	741	2	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.85e-208	5	734	8	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	1.24e-169	1	741	10	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	3.44e-169	1	741	10	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
6QUB_B	8.57e-169	4	749	7	857	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.18e-166	9	743	47	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.37e-97	9	733	51	811	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	9.29e-59	10	623	57	712	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	4.35e-55	9	731	8	725	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	6.18e-49	2	743	24	795	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999998	0.000024	0.000001	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004612_02167.