CAZyme Information: MGYG000004632_00654

Basic Information

• Species: Prevotella sp002300055
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp002300055
• CAZyme ID: MGYG000004632_00654
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
997		111402.88	4.8432

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004632	2937832	MAG	Germany	Europe

• Gene Location: Start: 28808; End: 31801 Strand: +

Full Sequence      

MNLKQYICLASLFLPIGTTAQTSNNHIICDFESEDSYTQVGENQWFDAVFPISGNPGAEL
HKLLVVPDLQSPHNRTSDFLFYIDDIELSTSATPRFSTTYYDINYEKTQKLNRTDRHLNS
ITLKADGKTQTLKVGQQSTKMLYMPMLEQAFTAKPGQTITATFNYQGTWMSGYVFLDRDN
DGQFNVVTDGDNITNDRDLMAYSFFKGKDSSGKTQPDGNRLNPPAFTLPSDLKPGVYRMR
FKVDWDNVEPGGSLTQDNSILSNGGAIVDVRLVVHNDQVTIKRGTRGTSGGLNGDVLKAD
GSKLTEELIPFGEPYNVSVKPAPGFKFSHFLIRHGNISSEDSLLYETPQFFDATVPSYLV
KNNLYTIPAKYIDGDVEITPYFKSTGSSQEGEGDYALNFNEELQRQGDNRLESFTMVATK
GGTTEVAIDETGNSVYRKMLDTEVSVVPGDQVAATASYTGSPMHGYLYVDLDNDGQFTAD
ISENGKPDVSSELLSYTYFNGKNSLGQNLSDADSAGVVLPAFTIPEDLPEGNYRARYKID
WDNASPAGQWTEGGENQIDAKGGYVVDFLLNVHPERSKLDIRTLNGYIMGKGNKGVSETI
AYGEQLALMTTPFNREYAAENMFIRHGHNLDGDQFICGNRQWSEDTLEVRNAYTLTKEQA
NGDVRITLEFEPVGNPVNKLVFSDEFNAPDGTYPDADKWVNPTRYSAAWNRFIAQTEEGR
RLTGYIKDGKLVMHCMPNPLETETDRNGQKLDMISGAVESHGKFDFHYGRVEARIKTNPY
TGNFPAFWMMPSKQPLGWPNDGEIDIWEQINTENKSYHTIHSKWGNTLGHANDPVKSGSA
ATQADEYHVFALQWTNKMLTWFVDGKKVFSYAKSKKTDALNNGQWPFDNDFYIILNQSVG
NGSWASAPDLGHTYTTLFDWVRVYQQVDPTGIGQTTTSAFDFYVGKNHVRLVAPEKQWVT
ICSAQGQVMYHKELQGNETVTLPHGVYVINKQKVVIP

Enzyme Prediction     

No EC number prediction in MGYG000004632_00654.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	683	924	1.2e-51	0.991304347826087

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08023	GH16_laminarinase_like	1.73e-56	682	924	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
cd00413	Glyco_hydrolase_16	1.27e-26	684	924	1	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
cd02182	GH16_Strep_laminarinase_like	8.72e-26	679	925	3	259	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.
cd02178	GH16_beta_agarase	1.19e-17	682	925	25	258	Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.
cd08024	GH16_CCF	2.01e-17	755	900	101	275	Coelomic cytolytic factor, member of glycosyl hydrolase family 16. Subgroup of glucanases of unknown function that are related to beta-GRP (beta-1,3-glucan recognition protein), but contain active site residues. Beta-GRPs are one group of pattern recognition receptors (PRRs), also referred to as biosensor proteins, that complexes with pathogen-associated beta-1,3-glucans and then transduces signals necessary for activation of an appropriate innate immune response. Beta-GRPs are present in insects and lack all catalytic residues. This subgroup contains related proteins that still contain the active site and are widely distributed in eukaryotes. Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic residues, like those of other glycosyl hydrolase family 16 members.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12302.1	1.07e-99	34	702	152	811
QFQ13169.1	1.28e-98	394	926	24	542
QUB87116.1	1.54e-82	676	995	46	333
AKU69476.1	1.54e-82	676	995	46	333
QUB63276.1	1.94e-75	680	993	50	331

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JH5_A	5.96e-28	680	928	6	235	Structureof Marine bacterial laminarinase [Aquimarina]
6T2R_AAA	9.86e-28	676	925	27	262	ChainAAA, Beta-glycosidase [Bacteroides caccae]
2HYK_A	1.15e-27	680	925	9	243	Thecrystal structure of an endo-beta-1,3-glucanase from alkaliphilic Nocardiopsis sp.strain F96 [Nocardiopsis sp. F96]
6JHJ_A	3.74e-27	680	928	6	235	Structureof Marine bacterial laminarinase mutant-E135A [Aquimarina],6JIA_A Marine bacterial laminarinase mutant E135A complex with laminaritetraose [Aquimarina sp.],6M6P_A Structure of Marine bacterial laminarinase mutant E135A in complex with 1,3-beta-cellotriosyl-glucose [Aquimarina sp.]
3ATG_A	5.11e-27	680	924	5	238	endo-1,3-beta-glucanasefrom Cellulosimicrobium cellulans [Cellulosimicrobium cellulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9ZG90	9.87e-34	679	925	57	288	Keratan-sulfate endo-1,4-beta-galactosidase OS=Sphingobacterium multivorum OX=28454 PE=1 SV=1
P45798	2.31e-28	679	928	41	286	Beta-glucanase OS=Rhodothermus marinus OX=29549 GN=bglA PE=1 SV=1
P23903	4.42e-25	620	926	366	681	Glucan endo-1,3-beta-glucosidase A1 OS=Niallia circulans OX=1397 GN=glcA PE=1 SV=1
C1IE32	2.09e-13	680	908	22	245	Glucan endo-1,3-beta-glucosidase OS=Cryptopygus antarcticus OX=187623 PE=1 SV=1
Q9Z3Q2	1.16e-08	726	924	278	457	Endo-1,3-1,4-beta-glycanase EglC OS=Rhizobium meliloti (strain 1021) OX=266834 GN=eglC PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000411	0.998820	0.000216	0.000179	0.000176	0.000161

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004632_00654.