CAZyme Information: MGYG000004633_02220

Basic Information

• Species: Erysipelatoclostridium sp002160495
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Erysipelatoclostridium; Erysipelatoclostridium sp002160495
• CAZyme ID: MGYG000004633_02220
• CAZy Family: GH29
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1552		170146.5	4.1295

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004633	2814686	MAG	Germany	Europe

• Gene Location: Start: 2167; End: 6825 Strand: +

Full Sequence      

MKNKKMMRKIAAAAMAGALVLPATVSSSKAQTLDGWVDDSQLNNSEVAAPEAYGPTPDAL
QYEYQKQELAAFCHFGPNTFNEIEWGENYGNTPPAEIFTLGPNDDFDAETLVETLKNAGF
KRLIVTAKHHDGFCIWASEYTKYDVASTNYKNGEGDILAEISKACSDYDMDMGLYLSPWD
IHDQSYGYFDKDGNALVGSDGQPLNGMSWEEVEEKDVYDYNEYYRNQLEEILGNDKYGNK
GEFVEIWMDGAKGSGYKTQNYNFDDWFATIQKYEGKEAGREADCLLFGSGAKTTVRWIGN
ELGFAPDTNWAQAKLIYKDGKATDIDTGWDESTGYAAGYPDGDVWTVPECDARITSGWFW
GNTKKTPKSMEELSEMYFRSVGHNGTLLLNVPPNDEGKLDKAIQERIVEFGNAVKNTFKT
NKAEDKAAYADTVLGNSKAYSASNVLDGDQDTYWTPEAGNSMGSVLIDLGETTNFDVVSI
EEAIQLGQRITNYKVEYSNDLQSWETMDEGTTVGSKRLSRTSPVKARYVKVTVSTNEDTS
PLISEIGVYKATGGLELNSSVPEGLTVVDNTDKDTSDGFGFTYNNSWVQEKGKDFINGTS
MYANEGSELTLNFHGTKAYLMGTLDPNHGKAMISVDNGPEVEIDTKASKRSVGNVLYASD
DLEDGDHTLTLRVVSDAIGIEAAAVIDNQGKGMFEFEHNAYRVLEAEDESATLEHKLTVK
RVGGTNGEAVVQVENNPGTAMQGNYDANLSGGTLTFAPGETEKQVSLYTKYDSRIQGDTS
FTMNLVNATNDVIIGFNKVATITIEDRDVKTKEQLAELIAQGEILNTQEYLYVSGWDAFQ
DAFKAAKETLADSDATIIQYGNAYRNLTVAINGLVVRDNYSEEDPFIMPVKKDETKVLEA
ELATLKNTGENEEYVLAVEERDWASNKKIINCFNAGDQIEVPYNASRAGKYKVVLTYRSG
SEENSFDVSEVDGKIKNVSVVAGNDDATVTKTKEFEIEVVEAGAGTLVLTAGTKNGPQLD
KLDITASDLNWKEFTIKATAGTGGTITPSGDNVYTELSDVTYTITPDNGYEIEDVKVNGE
SQGAITSYTFTELSKDSTIEATFKFKYYNESTPFEFPEKIGSENKVTLEAEKTELINTGD
ANEQWKLGIESGTWASGGQFVNCLNSDDQLVIHYNAPNAGIYQFTATYRSGSDTNKLVWS
GDKVISGEVAAGNSDASVVKTVDFLVEITEAGKGKIVFTAPSTNSPQLDKFDIVYFSDKE
SMARADLKALIEDAKAILENASDYQTATIENLKTVLAAVEDVYENDEASVEQLEQAKADL
QTAIDGLLEVVDKTELAQYIAKVQEIVDNIDDYSKYSVYGLEENLAKAKEVYNNENATEN
EVAAMVRTLKYGVENARKKAFIEDLEKLVAQAKAIDLEGYTPASVDVFELVLSDAQTLLD
SDLSFENQDSVDKMYDRLSKAINLLTKISDLEEDPGQTPDKPGDKPGSGDSGDNNNNGSQ
NPSNGNNDSSNPENGNASKTGDDINMTPFMVLMALSCLGAWGVLKRTKEIDY

Enzyme Prediction     

No EC number prediction in MGYG000004633_02220.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH29	58	415	3.4e-58	0.8641618497109826
CBM32	437	537	3e-19	0.782258064516129

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3669	AfuC	5.77e-63	49	549	3	430	Alpha-L-fucosidase [Carbohydrate transport and metabolism].
smart00812	Alpha_L_fucos	2.48e-27	53	412	7	328	Alpha-L-fucosidase. O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. Family 29 encompasses alpha-L-fucosidases, which is a lysosomal enzyme responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Deficiency of alpha-L-fucosidase results in the lysosomal storage disease fucosidosis.
pfam01120	Alpha_L_fucos	4.61e-24	48	412	1	326	Alpha-L-fucosidase.
pfam00754	F5_F8_type_C	5.83e-16	440	546	12	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00057	FA58C	3.57e-09	421	549	7	142	Substituted updates: Jan 31, 2002

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT29448.1	0.0	2	1399	9	1395
QHB23055.1	0.0	2	1399	9	1395
QEI30547.1	0.0	2	1399	9	1395
PLT74914.1	0.0	2	1278	9	1265
QPK81604.1	2.46e-293	28	1023	24	1001

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6TR3_A	6.02e-223	36	598	8	552	Ruminococcusgnavus GH29 fucosidase E1_10125 in complex with fucose [[Ruminococcus] gnavus E1]
6TR4_A	9.24e-222	36	598	8	552	Ruminococcusgnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1],6TR4_B Ruminococcus gnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1]
6ORG_A	6.34e-98	53	553	7	452	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORG_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6ORF_A	7.83e-97	53	553	7	452	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORF_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6OR4_A	2.66e-96	53	550	7	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6OR4_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_A Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8GW72	1.96e-89	40	549	18	477	Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2
Q7XUR3	9.65e-87	55	549	38	476	Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0560400 PE=3 SV=2
P49713	4.34e-07	64	417	34	345	Putative alpha-L-fucosidase OS=Caenorhabditis elegans OX=6239 GN=W03G11.3 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000286	0.998947	0.000208	0.000193	0.000182	0.000152

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004633_02220.