CAZyme Information: MGYG000004635_00426

Basic Information

• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Eggerthellaceae; ZJ304
• CAZyme ID: MGYG000004635_00426
• CAZy Family: GT111
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
626	MGYG000004635_40|CGC1	72344.88	4.5917

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004635	1613361	MAG	Germany	Europe

• Gene Location: Start: 1548; End: 3428 Strand: -

Full Sequence      

MTAIPTVKILVACHNEDVFVPELESLAPIQVGSACAQKRFGGMLHDDEGGNISYKNAMYC
EMTALYWAWKNLPEEDADYVGLLHYRRYLSFSPERFKTNYFGEAIFDRLDENTLNELNLT
DEAIAEAVKGYDVITSERGAFVGNPTMEQQWNESWQNNPDDLRLLRKIVEEVSPAMIPAY
DEYMASRSGYFCNTFIMHRPVFDDYCEWVFGVLQEHEKRADYSSYDAAQYRVSGYLAERL
TGVYITYLESKGAPVKELQRVFFEDASKPAEIRPAFGANAVTLVLASDDYYVPYLSALLQ
SVKDTSSPEREYDIIVLTRDIKASNQVLLKQQIEQKNVSLRFYDVSPLMRDYADALDLHG
HFQIETYYRLLMQDVLKDWNKVLYLDSDMIACRDIAELFDTDIDDYLLGAVRDADTAGLY
NGYNPQMKGYVDDVLGISDPYGYFQAGTILFNLDEFRKTFDISDIFDFATSKEWKLLDQD
VLNFIAQGCVKYVPMEWNVMTDWRGIRVKDIIARAPKWMRDEYLEARKHPAIIHYAGPEK
PWKDPSSDMADAFWEESRKTPYYEEALDRMLSQGKGGGLAGILKRGIPNDLWEQLYPVYQ
KLFPDRTRRREVMAYVYRKLVAGKKQ

Enzyme Prediction     

No EC number prediction in MGYG000004635_00426.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT111	9	246	5.1e-67	0.9861751152073732
GT8	279	544	2.6e-55	0.8832684824902723

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14393	DUF4422	5.63e-81	8	247	1	218	Domain of unknown function (DUF4422). This family of proteins is found in bacteria and archaea. Proteins in this family are typically between 255 and 371 amino acids in length.
cd04194	GT8_A4GalT_like	1.98e-68	281	544	1	248	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
pfam01501	Glyco_transf_8	3.85e-40	283	544	2	250	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
COG1442	RfaJ	3.21e-35	284	563	6	267	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
cd00505	Glyco_transf_8	4.61e-21	284	543	5	245	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT50573.1	6.69e-186	7	623	4	614
QPK94470.1	2.29e-157	5	570	5	573
BBV24392.1	5.35e-130	7	570	55	625
BBV24385.1	2.52e-128	5	562	7	569
QOL57823.1	3.00e-128	5	562	13	575

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U4B_A	1.30e-90	6	579	28	605	ChainA, WbbM protein [Klebsiella pneumoniae]
1SS9_A	3.15e-25	283	555	3	266	ChainA, alpha-1,4-galactosyl transferase [Neisseria meningitidis]
1G9R_A	1.44e-24	283	555	3	266	ChainA, GLYCOSYL TRANSFERASE [Neisseria meningitidis],1GA8_A Chain A, GALACTOSYL TRANSFERASE LGTC [Neisseria meningitidis]
5GVW_A	5.55e-06	328	557	38	251	Crystalstructure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVW_B Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVW_C Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVW_D Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae]
5GVV_A	9.70e-06	361	557	82	251	Crystalstructure of the glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVV_F Crystal structure of the glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P25148	3.05e-23	283	542	9	255	General stress protein A OS=Bacillus subtilis (strain 168) OX=224308 GN=gspA PE=1 SV=2
A0A0H2URH2	2.27e-13	282	569	4	256	Glycosyltransferase GlyF OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyF PE=3 SV=1
A0A0H2URA3	6.61e-09	279	554	2	247	Glycosyltransferase GlyB OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyB PE=3 SV=1
A0A0H2URH7	5.60e-08	294	554	313	538	Glycosyltransferase GlyA OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyA PE=3 SV=1
Q9AEU2	2.90e-07	311	554	14	250	Probable glycosyl transferase Gly OS=Streptococcus gordonii OX=1302 GN=gly PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004635_00426.