CAZyme Information: MGYG000004642_00922

Basic Information

• Species: UMGS1670 sp900548595
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Anaerotignaceae; UMGS1670; UMGS1670 sp900548595
• CAZyme ID: MGYG000004642_00922
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
750		87263.38	5.3292

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004642	2001820	MAG	Germany	Europe

• Gene Location: Start: 26508; End: 28760 Strand: -

Full Sequence      

MISTVSIYEMNLIVNCEHKDPHTVLGMHEVLHDDREVVAVRAFLPGAKELYVIDKNDENA
VYKADRIHEDGFFETVIEDRHKWFDYKLRIVYWDGNENITADAYSFPPTVSDYDKYLFGA
GNHYEIYNKLGANICEINGTEGVSFAVWAPNAKSVSVIGSFNYWDGRSAQMRMLGNSGIW
EIFIPGAAEFDRYKFRIKDRNNNVTDKSDPYGFYMEKRPQNASIVYDLGVYRWKDAKWIK
QRETSDPYRSPMNIYEVQLGSWMRVPEEENRFLTYRELADKLVKYVKKMGYTHIELLPVS
EYPFDGSWGYQVTGYYAPTSRYGEPDDFRYFVDCCHQKGIGVIVDWVPGHFPKDANGLAR
FDGTALYEHEDWRKGEHKEWGTYVFNYGRKEVSNFLIANALFWIKEYHIDGIRVDAVASM
LYLDYFRNEGEWIPNKYGGRENLEAVEFLKHMNSVIKGAYEGVLTFAEESTEWEGVTKGA
DRNGLGFSFKWNMGWMNDFLEYMKKDPIYRKYHHNNLTFGITYAYSENFVLVLSHDEVVH
MKGSMIGKMPGDIWQKFANLRAAYGFMYAYPGKKLLFMGNDIGQYSEWNEAKSIDWHVLE
NDFNCKLNLFLQDLFKLYKKEPAFWERDTYPEGFEWIECDDAENSVVSFVRHGANVEDLI
VIICNFTPKTVEGYDVGVPYEGYYKEILNSDDEKYGGSGVINKKAVRSKKEHCNRCANKI
TINLPPLATSVFTLTPNSREKLKKAVEEEE

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	285	584	1.5e-159	0.9933554817275747
CBM48	129	214	2.2e-18	0.868421052631579

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	111	732	8	625	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	0.0	105	734	1	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	0.0	13	732	511	1220	glycogen branching enzyme; Provisional
PRK14706	PRK14706	0.0	130	750	27	635	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	9	737	5	726	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMJ39890.1	0.0	7	745	17	751
QUH30965.1	0.0	9	732	9	722
ANV77640.1	0.0	1	732	1	729
ALX09866.1	0.0	1	732	1	729
ABN53393.1	0.0	1	732	1	729

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR5_A	3.51e-285	1	736	21	775	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR1_A	2.01e-284	1	736	21	775	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR0_A	2.84e-284	1	736	21	775	Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A	2.84e-284	1	736	21	775	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQW_A	2.84e-284	1	736	21	775	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8DLB8	2.77e-302	4	741	2	754	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q1AZ86	5.18e-289	13	729	10	714	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q3M473	1.14e-288	2	745	3	761	1,4-alpha-glucan branching enzyme GlgB OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=glgB PE=3 SV=1
Q8YYX9	1.61e-288	2	746	3	762	1,4-alpha-glucan branching enzyme GlgB OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=glgB PE=3 SV=1
P52981	1.31e-286	1	747	1	765	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000084	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004642_00922.