CAZyme Information: MGYG000004668_00839

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus
• CAZyme ID: MGYG000004668_00839
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
832		90177.15	5.1179

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004668	1185526	MAG	China	Asia

• Gene Location: Start: 25636; End: 28134 Strand: -

Full Sequence      

MHGKVSADQIDTTNQNNAQTTATVNTQNSASNNDNNNNGNNATSDVAADTSANSNNNTTN
NTAATSASSTANVATSNATTTNTNYDANDQGNYAYLDSVKLTGNGQVQLAGWNATNASQN
KPYHYIVAYDNTTKSEIAQTLVTNPVTRQDVQQAHNVYGAAQSGFNVTLDLGEDTLANAD
SISFISRYSSDQAGKVNNTDFWYAPITFDKENHAWLDSVAIKDGQLQVSGWNATNQAANK
PVHTVMVYDRTTKQVVASQVVENLDRSDVAKAYPDVVNAGKSGFFASFDLSQLNLNHQLQ
IISRYSSNTRSTGAATDYYFTPITNGNYTNQGSLDSFDLSDGSQLKVTGWHADDISKLES
NHFLILYDNTAAKQVGVVKATAVNRPDVAAVYKSVDTAAQSGFTGTFNLSNLNLILGHSY
SLVSRYSTSSNGNGGDGQYTDYWFKPVTLSQKAYNLDNVTMTNDGLHIVGWMASDYARGR
GNAYAIVLNNGKEVARQKLNLATRQDVANAYPSIYNSVKSGFDTTISLNPSQINGNMQVI
LRFAGSADGNSDFDDQYSKTYASNAGSFDAIQVSDSGIYVSGWHASNQAANMKYQYLIFV
DANSGKELYRQQVLDVNRTRNDIASAYPTILNSGQSGFQLGFTIPSELDHHTVKVIDRLS
DDVNGNGNYVDLTSDTVSIHNNKWAWPFPAVGEGHFMGAQLFGVNPGGEFRQNCFHDGLD
FGAYDHPGTQVHAIHSGTIVGVGYTAGLDWYVLEDTGEYLVVYQEAFSNRGNITVKPGQK
IEVGDVIGNRDTSHVHIGITRQHNFNIALAKSFTNDGTWLNPLEIIRNGLNG

Enzyme Prediction     

No EC number prediction in MGYG000004668_00839.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd12797	M23_peptidase	3.21e-13	716	799	1	85	M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins. This model describes the metallopeptidase M23 family, which includes beta-lytic metallopeptidase and lysostaphin. Members of this family are zinc endopeptidases that lyse bacterial cell wall peptidoglycans; they cleave either the N-acylmuramoyl-Ala bond between the cell wall peptidoglycan and the cross-linking peptide (e.g. beta-lytic endopeptidase) or a bond within the cross-linking peptide (e.g. stapholysin, and lysostaphin). Beta-lytic metallopeptidase, formerly known as beta-lytic protease, has a preference for cleavage of Gly-X bonds and favors hydrophobic or apolar residues on either side. It inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent. Lysostaphin, produced by Staphylococcus genus, cleaves pentaglycine cross-bridges of cell wall peptidoglycan, acting as autolysins to maintain cell wall metabolism or as toxins and weapons against competing strains. Staphylolysin (also known as LasA) is implicated in a range of processes related to Pseudomonas virulence, including stimulating shedding of the ectodomain of cell surface heparan sulphate proteoglycan syndecan-1, and elastin degradation in connective tissue. Its active site is less constricted and contains a five-coordinate zinc ion with trigonal bipyramidal geometry and two metal-bound water molecules, possibly contributing to its activity against a wider range of substrates than those used by related lytic enzymes, consistent with its multiple roles in Pseudomonas virulence. The family includes members that do not appear to have the conserved zinc-binding site and might be lipoproteins lacking proteolytic activity.
pfam01551	Peptidase_M23	2.70e-12	715	802	2	90	Peptidase family M23. Members of this family are zinc metallopeptidases with a range of specificities. The peptidase family M23 is included in this family, these are Gly-Gly endopeptidases. Peptidase family M23 are also endopeptidases. This family also includes some bacterial lipoproteins for which no proteolytic activity has been demonstrated. This family also includes leukocyte cell-derived chemotaxin 2 (LECT2) proteins. LECT2 is a liver-specific protein which is thought to be linked to hepatocyte growth although the exact function of this protein is unknown.
COG0739	NlpD	1.96e-05	599	799	51	248	Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contain LysM domain [Cell wall/membrane/envelope biogenesis].
COG4942	EnvC	0.001	702	788	311	388	Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLL76134.1	2.71e-166	84	713	123	762
QIZ04740.1	2.08e-165	84	681	113	727
QLL76139.1	3.23e-165	84	681	110	724
AYP74780.1	6.48e-165	85	713	116	754
AMY13042.1	6.77e-165	84	713	118	757

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GT1_A	1.89e-65	682	830	23	171	ChainA, Choline binding protein A [Ligilactobacillus salivarius str. Ren]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000042	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004668_00839.