CAZyme Information: MGYG000004672_01689

Basic Information

• Species: F23-B02 sp004558025
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; F23-B02; F23-B02 sp004558025
• CAZyme ID: MGYG000004672_01689
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2390	MGYG000004672_25|CGC1	263018.81	6.5653

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004672	1893728	MAG	China	Asia

• Gene Location: Start: 8232; End: 15404 Strand: +

Full Sequence      

MNKTTVFEHAASCARLNKCVSTAPVSELRALLRRSCRTINSAYRLVESRFGGKGSMPDSC
RWLLDNRYLAMRQARAAMPELRLQRRLRSGSEGMILAAACRDMITACGCSLSEDKILEYL
GGFQSVCPLPQNELYLIPAVLVTQLLSALAEICSELEQTAEPDALSTQFASLFGSLRLIS
ELDMHALLERTDAVEKALMADPSGVYPRMDEQSRAEYRSRLSRLAKREGLEEHAFAKQLV
ERCRHKDGAERHVGFWLYKRPDEKRRAALYIGANVLFTLFLSLLCGFLSRSVSAALLLLL
PLSELTKALLDYIILLCVPPRRLPRLELGSGVPDDGRTVCVISALLTDEDSGPRLAALLE
QYRLANRDCGENLLMGILADLREADSEDAPEDEAVISAAAEAIDALSRKYGGGFYLFTRG
RTLDSGRGKFSGFERKRGALLSLAQLMCGQNSELKTVSGDAELLRSARYILTLDGDTVPS
PGSVRELIGAMLHPLNAPMLDRKRGLITSGHGIIHPRMCTSLASAGATDFARIFSGGGGL
EAYGALCGEVSMDVFDRGGFSGKGIIDAEALLVCSEKHIPEGKVLSHDALEGAYLRGGYM
SDVEFSDSFPGSPIAYFRRSHRWIRGDWQNAGWIFRKSAELPDTERWRLFDSLRRSLVAP
ATFAAIFAGLLLAHGGLILAAWAALISLAAGLLISLTELASDKPEALAAKYHSRVLGGIG
ASLVQTAFRLWFLPYEAWISLSAIVTALWRMLVSGKNLLEWETAAQSGSKKLGISAYFKS
MWLAPVSGLCLVIFSAGVFAKAAGLLWIFAPIAAFALALPSKKEKEPSAQERNYLLGCAK
DIWSYFDSFCTGQDNFLPPDNFQEQPPVGIAHRTSPTNIGLALCSAMCAQELGIIDLPRA
VSFIENMLGTMEKLQRYSGHFCNWYDTRTLRPLEPKYLSAVDCGNLCACLIALQSWLHAK
GLDTLADRVQALISDMDFSIFYSARRGLMHIGIDLEKGAASPGLYDLMASEARLTSYAAI
AKGDVPRRHWRRLSRAMRSSGRYRGMASWTGTMFEYLMPELFLPLTRDSLLFETAKFCVY
VQKKRRSRGGLWGISESAFYSLDPALNYRYKAHGCAQLALKRGQDTELVIAPYASFLALG
VDPDAATENLHRLEKAGLRARFGFIEAIDFTHSRCRDCGGEKVRCFMAHHLGMSMLAITN
YLSRGFVQHEFMSSPDMAAHRQLLQERVPLGAPVLKLGEASEKSPAPLSRQWEKRSGSIN
YTQPECSLLSNGAYNIMLTESGIVSANCNDTLIYRTPFKQLGEGHGAELRLAIGGRERSL
LPEAGESAAFMWEFSEISANFSAVFDDLASQCGIAAAGAENGEIRTVSICAKSDIESAEL
RLSFEAVMADASDYVNHPAYWRLGIEAKRDQNCLMLHRLPRGRQREAWLCLACDKPMAAK
TGGSADDGFLSCPTVEAAVNFSLKAGEQLSARFALCAADSPEDAYSGAQHMLVIGPSEYG
AMVSACASVTHMSAREVESAMTMLQNLRFISAKTPFPAKSSLWRCGLSGDIPIICCDGAQ
NVESVTKQFCLLRSCGVYADLVFLTDEGGEYSRPVYSKVRDILTAHGLEALIGTHAGVRM
LPTQEASLIKSAAAVIVGEDREKRSFDTAPLPSKPHRTVGALPRHEYGDGGSFMFYVNRA
LPARVWSSILTNGSFGYIAADSGMGNMWLKNARELRITPWMNDPTAIEGAESLEYVTDSG
RYSLFADEDGAACRVTFGLGFAVWEKSFGDCASRCTAFVPPNLDARVFIIKLFGEANGRL
DWCCELLLSDNDNDRAAVSCEYAENMFTASSSRSAIRNLSFRAACSSPALAWTCDGLSRL
YGELDGRTHELGFPVFAAQYEAKAVTVLVCGCESADAILKLCEPDAAFAALEKTKAHWSK
LCRRFTLAGCGSADKYMSGWAVYQTLACRIMGRCSVYQSGGAFGFRDQLQDAVNLILLDP
GIAKQQILACCRHQYLEGDVMHWWHSIGGADKGVRTRCSDDLLWLVWALCEYVEKTGDED
ICGRLEYYVSSKPLDADEEDRYETPLRSDCSESVLMHAQRAVDRCMMRGTGSHGLLRFGS
SDWNDGMSNIFGESVWLSWFFSCCVKRFADLLVELGGSDADKYRSFAADIGRAADRAWNG
QWYLRGYWPDGEELGARQNECCRIDSIAQSWACFCPEASNTKADAALDAALRLLFDRENG
IVKLFDPPFFGDGRDPGYIRSYGPGFRENGGQYTHAALWLAQACFKRGRANDGWAILQCI
LPENHDLRRYTAEPFVIPADVYTCPGHIGEAGWTWYTGSSGWYFRVFTEELLGLKPWHGM
IYIRPCLPDDFPDCRVTLKTKTGKTHDIMIGQSGIWLDGAIYDGKGIPYI

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1538	2388	3.9e-166	0.8561776061776062
GT84	1004	1215	1.4e-76	0.9906976744186047

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	1.30e-121	1500	2389	97	1040	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	6.43e-81	1917	2335	1	425	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
pfam10091	Glycoamylase	4.53e-21	1005	1215	3	215	Putative glucoamylase. The structure of UniProt:Q5LIB7 has an alpha/alpha toroid fold and is similar structurally to a number of glucoamylases. Most of these structural homologs are glucoamylases, involved in breaking down complex sugars (e.g. starch). The biologically relevant state is likely to be monomeric. The putative active site is located at the centre of the toroid with a well defined large cavity.
cd11753	GH94N_ChvB_NdvB_2_like	4.41e-17	1208	1421	2	225	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
cd11756	GH94N_ChvB_NdvB_1_like	1.36e-16	1669	1901	14	279	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBB66089.1	0.0	29	2388	34	2385
ALP93881.1	0.0	29	2119	34	2105
CDZ23197.1	0.0	32	2380	36	2479
BCI59942.1	0.0	28	2348	32	2423
AVQ95160.1	0.0	23	2374	36	2524

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1V7V_A	1.60e-42	1905	2388	287	787	Crystalstructure of Vibrio proteolyticus chitobiose phosphorylase [Vibrio proteolyticus],1V7W_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc [Vibrio proteolyticus],1V7X_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate [Vibrio proteolyticus]
2CQS_A	1.07e-36	1909	2389	326	829	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]
3AFJ_A	1.42e-36	1909	2389	326	829	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127],3AFJ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127]
3S4C_A	3.94e-36	1903	2389	303	809	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]
3ACT_A	7.54e-36	1909	2389	326	829	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127],3ACT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	6.69e-263	160	2372	273	2807	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
Q76IQ9	8.39e-42	1905	2388	287	787	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1
B9K7M6	1.56e-35	1910	2388	301	798	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1
Q9F8X1	1.93e-35	1901	2375	283	767	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1
Q8P3J4	1.81e-22	1966	2381	362	775	Cellobionic acid phosphorylase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=XCC4077 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000051	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
267	289
656	673
678	700
727	749
788	810