CAZyme Information: MGYG000004678_01273

Basic Information

• Species: Finegoldia magna
• Lineage: Bacteria; Firmicutes_A; Clostridia; Tissierellales; Peptoniphilaceae; Finegoldia; Finegoldia magna
• CAZyme ID: MGYG000004678_01273
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
780		87027.13	9.6892

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004678	1841606	Isolate	United States	North America

• Gene Location: Start: 71609; End: 73951 Strand: -

Full Sequence      

MKNLKDIAKVSFVALSVSLMISTGSMAQTENDKKDVENELSAKYTSISTHNQSKLTKKAK
LGYANVDNLQNEKKENGDLNHEDLKSTDSNKDIKEKSEKKEASESTKDEEETEKEVVSEK
SDIKDTEEVLEDSPIKPEKSVSKENSEEQVSEEADDKKAQDEEVKALNSAGQIIPSRIAG
QNRYESAAQISREQFTNAKKVIVVNAQKYADALSATTLSDGKYSILYTEKDSLPTATRNE
IQRLNPVEVYLLGGQQSISSGIENILKKYSNKVTRIAGRDRYETSAKVAAMSKKKNVVIA
SGENFSDPLYASSYAYSNNAKILLSSGKTLSRQTRDYLLRNKSSIGKVTVVGGGQSISSA
TVRYIQSVTGKNVSRISGRNRYDGSVKVANSMNKDKVFIASGEDFADALAISPLAQKLNA
PILLSSRSKLDTSVIAFLNNFKKSIKDVYIVGGYRTIDNNVYGTVKNVLAKPVKKPQAKP
QPKPPVVKDQSDIIEEDKAIGLHDFVVAKHSTKLYNAANSSAKSVRDINTSTVMQVINIL
NNGWMQLDINGFKGYAKIDDFGMFNPNKYRLVFQQGADISKFNRNVNMKLAKQNGMDFVI
LKAGSGYSGEDPKFQQNYRNAKAAGLNVGAYWYSYAVNVEEAKEEAVRYLKILGKKQFEY
PVYLDFEDPSQRRIPTKTKTDMAIAFMSILEKNGYYTGLYSSGYWINNQFEKNRLKDYDI
WIAHWHVTSPNCFTPDYGMWQFTNKSKIKGVPDTGEGGVDMNYSYKNYPKIIKNAKLNNF

Enzyme Prediction     

No EC number prediction in MGYG000004678_01273.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	578	751	3.9e-44	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06414	GH25_LytC-like	2.01e-75	574	765	1	191	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
cd00599	GH25_muramidase	8.52e-38	575	763	1	185	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
pfam01183	Glyco_hydro_25	1.01e-31	578	751	2	180	Glycosyl hydrolases family 25.
cd06525	GH25_Lyc-like	3.59e-28	575	763	1	183	Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by the lyc gene. Lyc has a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
pfam04122	CW_binding_2	9.71e-23	375	460	1	80	Putative cell wall binding repeat 2. This repeat is found in multiple tandem copies in proteins including amidase enhancers and adhesins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAG07790.1	0.0	1	780	1	780
QKH79509.1	0.0	1	780	1	785
QQK08229.1	8.37e-51	576	780	5	217
QAR21437.1	8.79e-44	575	780	5	206
QQO42089.1	1.20e-43	575	780	5	206

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5J72_A	5.84e-28	177	452	134	419	ChainA, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630],5J72_B Chain B, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630]
5J6Q_A	2.06e-21	178	426	289	551	ChainA, Cell wall binding protein cwp8 [Clostridioides difficile 630]
7ACZ_B	1.11e-20	140	429	3	341	ChainB, SLPH (HMW SLP) [Clostridioides difficile R20291],7ACZ_D Chain D, SLPH (HMW SLP) [Clostridioides difficile R20291]
7ACX_B	4.08e-20	178	426	43	329	ChainB, S-layer protein [Clostridioides difficile],7ACX_D Chain D, S-layer protein [Clostridioides difficile]
7ACY_B	7.36e-20	178	426	43	329	ChainB, S-layer protein [Clostridioides difficile 630],7ACY_D Chain D, S-layer protein [Clostridioides difficile 630],7QGQ_B Chain B, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_D Chain D, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_J Chain J, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_K Chain K, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_L Chain L, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_M Chain M, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_N Chain N, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_T Chain T, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_U Chain U, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_V Chain V, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_W Chain W, Precursor of the S-layer proteins [Clostridioides difficile 630],7QGQ_X Chain X, Precursor of the S-layer proteins [Clostridioides difficile 630]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q02114	2.80e-32	177	467	30	315	N-acetylmuramoyl-L-alanine amidase LytC OS=Bacillus subtilis (strain 168) OX=224308 GN=lytC PE=1 SV=1
Q02113	2.85e-25	174	423	61	302	Amidase enhancer OS=Bacillus subtilis (strain 168) OX=224308 GN=lytB PE=1 SV=1
P26836	8.20e-15	575	779	10	211	Probable autolytic lysozyme OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=lyc PE=3 SV=2
Q8X7H0	1.84e-11	535	762	30	252	Uncharacterized protein YegX OS=Escherichia coli O157:H7 OX=83334 GN=yegX PE=3 SV=2
P76421	4.49e-11	535	762	30	252	Uncharacterized protein YegX OS=Escherichia coli (strain K12) OX=83333 GN=yegX PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000402	0.998832	0.000270	0.000160	0.000162	0.000153

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004678_01273.