CAZyme Information: MGYG000004680_02759

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lacrimispora
• CAZyme ID: MGYG000004680_02759
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1169	MGYG000004680_49|CGC1	132592	5.1781

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004680	3596809	MAG	Germany	Europe

• Gene Location: Start: 14874; End: 18383 Strand: -

Full Sequence      

MCKKIYDWGTHLFCDDWFFFKESPPTLTDKFPPVTGFQPVGLPHDWLIEQGNDLYENSTG
WYRKCMYFQTMEDRIALLRFDGVYMDSTIYVNRKKAGEWKYGYSCFEVDMTPWLNDGENE
ILVKVNHQSPNSRWYSGAGIYRDVWLKVLHRDRLISDGLYVSAGKKEDQSWELEISAEVL
SLSGGTIGFYLKEREEEEGAEDLFLGEYKIKAEEGVQTVTAVAGISSPKQWDIESPVCYS
LRAELFRGGEKLQTQWVRIGFRTVEFHPDLGFLLNGRKVKLQGVCEHHDLGCLGAAYHSQ
AMRRKLTILKAMGVNAVRISHNMPSQDVMELADEMGILIVSEAFDMWERPKNPYDYARFF
PEWYPKDVASWVRRDRNHPSLIMWSIGNEIYDTHADERGQEVTRLLMEEVKKHDPRGNAR
VTIASNYMPWENARKCADILKLAGYNYGEKYYDAHHRAYPDWVIYGSETASTVQSRGIYH
FPYSQSLLGEEDEQCSSLGNSTTSWGASSSEACILAERDHPFSCGQFLWTGFDYIGEPTP
YHTRNSYFGQVDTAGFPKDSYYIYQAEWTDYKKAPMIHVFPYWDFNDGQRIDVRICSNAP
AIELFLNGVSQGKCVLDHKNGDRLTGNYRIPYEKGVLKALAYDESGNVIAEEVRSSFGEA
ESISLQAEKEYLKSNGKDLLFVTVSMKDAQGNPVENANNRIFITVSGSGSLVGLDNGDST
DKEEYKTGSRRLFSGKLLVIVVAGTKAGELRITASSPGRKEEVLTVPVVEGEAEEGSSAL
AYRVSEKILKQERLEDRLTDIPVRNIKLTSVPGTRLTKDQKTAVVSARICPEQALDQTLF
WSVVDDRGIPSKLAALTVQGNQACVTAQSDGHFRLRCMSRSGTEKIRILSTLEFTVEGVG
ETFLNPYEFVSGGLYDYGVGEIGNGNEKGVSTARDGETHVGFHNLSFGSGGSDEITIPIF
ALTGEPYRIRIYEGMPGEGSAKLIGDVIYQKPSIWNVYQEETFHLTRKLRGITSLCFVLE
KKVHIKGFIFTRKSRGFDRLFAGECDGVYGDHFKKEQEEIREIGNNVTIRFEDMDFGDKG
TDCIRIWGYTSNKTNSILVRFLGEEGDAHGEGEQEQMLEFQHAEEPEDQFFAFDRVTGTR
TVEFVFLPGSHFDFRWFQFMDSRKGNRNM

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	35	567	1.2e-98	0.5797872340425532

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	4.46e-59	13	720	55	767	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	1.83e-35	663	765	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	2.52e-32	61	415	68	441	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.87e-29	56	389	107	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.97e-24	56	389	118	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJU15650.1	0.0	12	1159	2	1144
ASU31293.1	0.0	12	1159	2	1144
ANU74303.1	0.0	12	1159	2	1144
QQQ91838.1	0.0	12	1159	2	1144
AWP27978.1	0.0	12	1160	6	1151

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	2.36e-164	13	766	6	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	7.91e-164	13	766	12	805	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	1.41e-148	13	768	30	820	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CU6_A	1.84e-144	34	766	40	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	5.03e-144	34	766	40	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	4.77e-159	40	770	74	850	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.78e-93	13	736	51	788	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.39e-52	37	732	79	787	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.87e-35	16	762	11	731	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
Q9K9C6	7.49e-24	56	391	118	462	Beta-galactosidase OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000030	0.000005	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004680_02759.