CAZyme Information: MGYG000004705_00589

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485
• CAZyme ID: MGYG000004705_00589
• CAZy Family: GH18
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
352		40401.33	8.3274

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004705	2427413	MAG	China	Asia

• Gene Location: Start: 158204; End: 159262 Strand: -

Full Sequence      

MNHRFHCIVRGTILLLSIIFLCPCLSACHRKEKAVDGKPKISVAYVQAQRAGVPDPNLFT
HFIYSFAVFNDDNDGVVVEYPEKLKAMSGLKEENRNVKIILGVGGLKREGFSEMAGDKTK
RKRFVENCARIIKEYNLDGIDLDWEFPTTEKGGHTATPDDDVNYGLVVKELRKKLGKNKW
ISFYSNNTANWIDFDRMLPYVDYVNVSGYNLDMPKPDKPLLHQSRLYKSKRWGKWCVEES
IKAHVKKGVPVEKILLGIPFYARGKKPFPDYLEGKNYSRYDGYAQLEWDDEAKVPFLTDE
EGNMILGFDNEQSIQAKCDFIKEYGLAGAFVWSYNADYEDFRLSKALKIGLR

Enzyme Prediction     

EC	3.2.1.14

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	55	340	6.3e-58	0.8513513513513513

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06548	GH18_chitinase	7.72e-74	55	337	22	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
smart00636	Glyco_18	4.37e-67	55	337	22	334	Glyco_18 domain.
pfam00704	Glyco_hydro_18	7.95e-67	49	337	13	307	Glycosyl hydrolases family 18.
cd02872	GH18_chitolectin_chitotriosidase	3.48e-58	55	342	24	344	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.
COG3325	ChiA	2.03e-40	30	338	29	424	Chitinase, GH18 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SCD19272.1	1.69e-82	36	347	31	337
ATP56025.1	3.28e-82	40	351	34	339
QRO23424.1	4.36e-81	13	352	8	335
SNV43048.1	6.55e-81	2	352	4	338
BBB35952.1	7.09e-80	28	351	28	346

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6INX_A	9.63e-45	60	352	28	343	Structuralinsights into a novel glycoside hydrolase family 18 N-acetylglucosaminidase from Paenibacillus barengoltzii [Paenibacillus barengoltzii]
6TSB_AAA	2.34e-43	55	351	50	350	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
6T9M_AAA	2.52e-43	16	351	14	369	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
3QOK_A	3.73e-34	55	340	50	406	ChainA, Putative chitinase II [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
6HM1_A	5.21e-34	55	351	27	393	Structuraland thermodynamic signatures of ligand binding to an enigmatic chitinase-D from Serratia proteamaculans [Serratia proteamaculans 568]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20533	1.04e-28	84	351	142	451	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1
Q9W092	2.97e-27	55	342	66	406	Probable chitinase 2 OS=Drosophila melanogaster OX=7227 GN=Cht2 PE=1 SV=1
Q9D7Q1	8.75e-27	55	342	47	364	Chitotriosidase-1 OS=Mus musculus OX=10090 GN=Chit1 PE=1 SV=2
O35744	1.93e-25	8	341	6	367	Chitinase-like protein 3 OS=Mus musculus OX=10090 GN=Chil3 PE=1 SV=2
Q13231	2.78e-25	55	341	47	365	Chitotriosidase-1 OS=Homo sapiens OX=9606 GN=CHIT1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000068	0.001410	0.998525	0.000004	0.000005	0.000005

TMHMM  Annotations     

start	end
7	29