CAZyme Information: MGYG000004706_01420

Basic Information

• Species: Olsenella_B sp900768455
• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Atopobiaceae; Olsenella_B; Olsenella_B sp900768455
• CAZyme ID: MGYG000004706_01420
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
622	MGYG000004706_3|CGC8	69692.67	5.7485

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004706	2415363	MAG	China	Asia

• Gene Location: Start: 357790; End: 359658 Strand: +

Full Sequence      

MDIRRMLAGRLRTHEREDPLPLWTPWGEELLARDEKGAGLGGPSDVNRASSPATHPHPQF
ARSAWGSLNGWWECAFVASPGGDAPERTAAVPATFSQHIRVPFSPEALLSGVGRQLKPDE
LLWYRRSFEAPAIPAYERLLLHFDAVDYACACYVNGDLAGSHAGGYLPFSFDVTDLLMPG
EKNELALCVRDPSDTEPHLRGKQLLQCGTIWYTAQSGIWQGVWWERVPENRIEELLVDAS
PDAGDVTVSVWVVRPGKELRVSLLSAEGALLARGAAVASEKSVAVRVPVKVPHLWCPDDP
YLYGLRIEYDADVLTSYCAFRTVSVEPDATGVPRFCLNHQPLFLRGLLDQGYWSDGLLTA
PSAGALEADIKAAKDLGFNMLRKHIKVEQDRWYWLCDRLGMLVWQDMVSGGTAPYDMWQV
NQKPTISRFACGHFRDDTASHQARLSSADPAFQEEWTRDCEGTVRYLRNHPCIVTWVLFN
EGWGQFDARDAVRRVRAIDPTRPIDATSGWFDQGCGDFVSEHNYFRKLRVHLPRGSSRAF
VISEFGGLTWYVKGHSSFSEPYGYAGFPSLEEWRRAVRALLDEARALEAKGLSGYVYTQL
SDVEEETNGLLSFDRRVNKLLS

Enzyme Prediction     

No EC number prediction in MGYG000004706_01420.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	54	521	3.9e-102	0.5053191489361702

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.21e-40	123	504	66	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	2.12e-36	66	504	29	445	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.82e-19	63	502	39	470	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.19e-13	143	504	144	485	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	9.28e-13	71	194	23	138	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACV23240.1	5.58e-244	1	621	2	600
VEH02373.1	5.58e-244	1	621	2	600
BAK45232.1	4.58e-243	1	620	2	609
ACV55172.1	1.79e-239	1	620	2	610
QOS69434.1	6.34e-233	1	620	2	615

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	4.93e-113	23	620	5	570	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
4JKM_A	1.67e-21	68	481	18	415	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]
3K4A_A	9.23e-21	71	521	36	460	Crystalstructure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12],3K4A_B Crystal structure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12]
6U7J_A	1.57e-20	122	507	77	448	UnculturedClostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_B Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_C Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_D Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.]
4JHZ_A	2.84e-20	71	521	36	460	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12],4JHZ_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	2.65e-24	123	570	59	479	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KM09	7.07e-21	123	509	109	459	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P05804	4.81e-19	71	521	34	458	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
T2KPJ7	3.68e-18	123	481	106	430	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
A5F5U6	7.41e-18	124	545	121	521	Beta-galactosidase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000060	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004706_01420.