CAZyme Information: MGYG000004707_00353

Basic Information

• Species: Monoglobus sp900554105
• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales; Monoglobaceae; Monoglobus; Monoglobus sp900554105
• CAZyme ID: MGYG000004707_00353
• CAZy Family: PL11
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1400		150724.56	4.1924

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004707	1967109	MAG	Denmark	Europe

• Gene Location: Start: 13593; End: 17795 Strand: -

Full Sequence      

MRLKSKLLSMTLALVMVICTLPALAAGNADTSEPIISTATNEPRQMEYLNRGGFAATNPS
GGMYLSWRLLGTEPMDTTFNIYKNGGRLVTELDNTNYTDATGTAGDEYTVAPVINGIEGA
QSEPMMQLTGYMDSSWKTSPYAYFDIPLQIPPAGSSYTYSPNDASVGDVDGDGEYEIILK
WNPSNSKDNASSGVTGNVYIDCYEYDGTFLWRIDLGRNIRAGAHYTQFQVYDYDGDGKAE
VAMKTAPGSIDGKGKYVSDAGNTDAIKATDNSTSYVNSSGHIAGGPEFLTIFNGETGEAM
QTIEYDPLQSTGSWGDSKYNRSERYLAGTAYLDGVHPSMIFCRGYYARSVAVAYDWDGVN
LTKRWKLDSLDSGNSGFAAQGNHQLSVADLDNDGKDEIIYGAAAIDDDGSLMWSVYYKDV
KLGHGDALHVSDFDNDGAQEVFKVNEDHPNWGRCLINADGTLEWMQVATGDDGRGVMANF
SAKYGVLAWDSGTNVRTLGGTVVNNNVLFDNGQSYPNFPIYWDGDFYREHYDKCRIQKWY
DYEEADTDGNLGSFGRILGFSGVTYNNDSKQNSCLQADLFGDWREELIFRTSDDSALRVY
TSLIPTEHKLTTFMHDSQYRCAIAWQNTGYNSPPHTSYYIGEDKTAYEQPNIETVTLKPE
IRLTVTADGNPVSGIYVKLGDTEKPTDSDGVVSFRVNPGTYAYTVDQAGYKIASGTVVHA
DGVDPTEQTIILETLPDSTITVVSDGSPVSGATVVIEGQTAVTDENGNVTVKLRSGQNSY
TVSCHKYITKTGTLSVPEAGTVESIEIEPVQYEYDSTKDADGSKFTYSGGDGAALSFSGG
TWTFSQNSTDGGRSFGATFDTSADGNMEFEMTYGTGGQKGSNGDWSWTGRAYTHEIKLLD
INGNILIGLSQEYLESGAQQVQYYTGSKSKTNVSTGNMIGAPNITARSASTWAIKFSVDF
KAKTVDLTLTDEAGANGYVITDIPINVTNFSKVTIGSTASGNVTWAPTLKDVLYYSDCIN
NSIGTATPAPTPIKKYDVTVSKSGEGEVGLVDNVVEWNFGAEPFVNSDGTVNTALFPAGT
TEGRYCLINTSIKNVNGLTYVTDITSASDKIANNANQSSPKTFIDGYNGYRMIKTQTAGS
TSNYYFTFTPDSDGIVNVYARSGSGSASAKLYIVQGSSTKGTMTLPDTSSEAANGSLPVL
SANVSANEAVKIYSDSNTAFYAITFNPENTTALTTTEQIVDTLSFKEGDTVTVKAVAANQ
TDDVTVTTDTNAQVTAVDGKAGYYTFKMPAENTVVKAEFKANDNNPYTINSVLPSVDGVN
VNLTNNSDTGAEPAKVIVAAYDSASGMLKNIGIEDASIPTGESGDVYVPITYSDSDLVIA
YVWNSMSDIVPLATSYKLSN

Enzyme Prediction     

No EC number prediction in MGYG000004707_00353.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	44	653	1.3e-218	0.9965694682675815

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	0.0	46	638	1	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
pfam18370	RGI_lyase	6.83e-28	44	124	1	82	Rhamnogalacturonan I lyases beta-sheet domain. This is the beta-sheet domain found in rhamnogalacturonan (RG) lyases, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 carrying this domain, such as YesW (EC:4.2.2.23) and YesX (EC:4.2.2.24), cleave glycoside bonds between rhamnose and galacturonic acid residues in RG-I through a beta-elimination reaction. Other family members carrying this domain are hemagglutinin A, lysine gingipain (Kgp) and Chitinase C (EC:3.2.1.14).
pfam13620	CarboxypepD_reg	0.001	661	732	2	81	Carboxypeptidase regulatory-like domain.
PRK15223	PRK15223	0.007	701	889	645	833	fimbrial biogenesis outer membrane usher protein.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUO19809.1	0.0	4	1395	2	1329
AIQ75296.1	5.66e-187	44	655	7	588
QKS43655.1	2.63e-186	44	726	43	700
AWV36810.1	2.89e-186	37	655	18	606
QVN28362.1	1.02e-183	39	654	35	619

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z8R_A	2.65e-181	44	654	3	582	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
4CAG_A	2.40e-180	42	654	7	589	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2ZUY_A	3.08e-173	44	663	6	612	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	2.73e-180	39	654	35	619	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	1.30e-172	44	663	6	612	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001132	0.997458	0.000340	0.000380	0.000334	0.000320

TMHMM  Annotations     

start	end
7	29