CAZyme Information: MGYG000004708_00455

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000004708_00455
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
711	MGYG000004708_22|CGC1	80302.29	7.5881

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004708	1820911	MAG	China	Asia

• Gene Location: Start: 16731; End: 18866 Strand: +

Full Sequence      

MKHILLKTLKQSVSNSRTHYLGGLMLFLSVPALAQQNGITDTRNSKFAIMNSTPISSVKW
TDGFWAERFNVLSGTSLQSMWDTWRSPDGHSYNNFLIAAGEKQGKRHGPPFHDGDFYKWL
EAVAAVYAINKDPELDKIMNEVIRLIAKAQRPDGYLHTPVIIAELNKKAKQQEDEKENEA
IGTKVGTGKDGAFGNRLNFETYNLGHLITAGIIHKRATGKTTLFDCAVKAADFLSGFYEN
NPDELARNAVCPSHYMAMAEIYRETGNPRYLKTAQGMIDIRGKVENGTDDNQDRIPFRQQ
YNAMGHSVRANYLYAGVADLYIEQGEGQLMKNLTSIWDDIVYRKMYINGACGALYDGTSP
DGTDYKPDNIQKVHQSYGRPYQLPHSTAHNETCANIGNMLLNWRMFQATGDAKYTDLVEN
CFYNSILCGISLDGTKYFYTNPLRLSDELPYKLRWSKEREVYIKRCFCCPPNTLRTLCEA
QDYAYSVGKNEIYVNLYGANTLTTTLEGIGKISLKQETDYPWDGKIKITITELKGKKDLT
FKLRIPEWADVAEVKVFGKRGNEMDVKTDAGGKYVSVPYTWQKGDVITLDLPMKTKIIEA
NPLVEECRGQVAVQRGPVIYCLESNDLNGLDIDNIAIPADAKITPVEVNIAGSRMVALDT
EAVNRSESSWEKTLYREFNKDNKKAKIRLIPYFAWGNRGKSEMTVWMPVEF

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	58	621	3.2e-180	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	57	621	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	1.36e-127	63	707	21	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABR40298.1	0.0	32	709	18	696
QQY37562.1	0.0	32	709	18	696
QEW37287.1	0.0	32	709	18	696
QQY43428.1	0.0	32	709	18	696
ALK84932.1	0.0	32	709	18	696

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQO_A	0.0	32	709	18	696	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
4QJY_A	4.67e-119	56	707	14	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	1.18e-115	50	710	7	626	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QK0_A	1.80e-114	56	707	14	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
7EXU_A	8.64e-78	90	705	52	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.01e-76	90	705	52	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.259914	0.716284	0.020403	0.002392	0.000552	0.000436

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004708_00455.