CAZyme Information: MGYG000004719_01343

Basic Information

• Species: Roseburia sp900756925
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Roseburia; Roseburia sp900756925
• CAZyme ID: MGYG000004719_01343
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
814		88721.57	4.9042

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004719	2432384	MAG	China	Asia

• Gene Location: Start: 1007; End: 3451 Strand: -

Full Sequence      

MEKYLDKSMSPKERAEDLLAKMTPEEKLAQTQCVLVPEAKVDLAKGFLTAGIGEISTLDV
RNIEDRHRAAAFINKVQDIVMESSPHRIPAIFHMEGLCGAFVQDATSFPSGIARASSFNP
ETERKIGAIVARQENAAGITHTLAPVLDISRDSRMGRQGETYGEDPALASAMGSAYTKGI
QETPVVIDGDERHSEAVAKHFMGFHNSLGGIHGGDSMTTPRLMEEIYGRPFQAAITEANL
RGVMPCYCTFDGEPASASKHLLTDLLREEMKFDGLAVSDYGAVENVHNVQKAYETVTDAG
EACMKAGMDVEMPMPSGYNKELLERMKSGEADMQILDTVVERILEAKFRMGLFEHPYALT
GEKFDQVFFDEKDKEVTLQSATESLILLKNDGALPIDKSVKKIAVIGPHANNARDFFGGY
THISMVEAVHAVANSLAGVRKSKDEKPKEVKYVPGTQIQSNETEEFEQVLKTIKPGCRNL
VEELCATLPDVEIVYAHGYEIAGADESEFAKALEAVKNADLCILTLGGKHGSCSVASMGE
GVDATDINLPPCQDRFIEEAAKLGKPLVGVHFNGRPISSDVADEKLNAILEAWNPSEAGA
RAIVSVLTGEENPSGRLPVCVAYNAGQIPIFYNHVNGTSWHQGASIGFTNYVDMPHTPRY
FFGYGLSYTTFNYSNLKVDKESMTADGSIHVSVDVTNTGSVAGTDVVQLYVRDVFASMVR
PEKELAGFARVLLNPGETKTVTFDLKATQLAFLTTDMKWKVEKGAVDVMIGKSCDDIALA
TQVQIENDAYIDGAKRRFYAVSEIIRVSPLPSHN

Enzyme Prediction     

No EC number prediction in MGYG000004719_01343.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	82	313	3.3e-58	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	2.22e-144	16	771	39	751	beta-glucosidase BglX.
COG1472	BglX	5.25e-76	22	421	1	372	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	4.68e-58	23	346	1	316	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	4.64e-57	102	743	108	740	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.18e-41	385	668	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VCV22852.1	0.0	3	807	7	819
CBL11432.1	0.0	3	807	7	819
QRT50982.1	0.0	3	807	7	819
AJG98232.1	0.0	1	800	5	801
AHF25953.1	0.0	1	804	1	798

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5Z87_A	1.71e-112	4	777	36	777	ChainA, EmGH1 [Aurantiacibacter marinus],5Z87_B Chain B, EmGH1 [Aurantiacibacter marinus]
5YOT_A	2.26e-110	3	777	6	748	Isoprimeverose-producingenzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]
7EAP_A	1.69e-109	3	777	6	748	ChainA, Fn3_like domain-containing protein [Aspergillus oryzae RIB40]
5XXL_A	2.33e-96	18	780	17	747	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
6R5I_A	9.24e-96	18	771	10	719	ChainA, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5I_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KMH0	2.46e-114	88	777	65	715	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
Q56078	7.53e-106	17	771	40	751	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	2.06e-105	17	771	40	751	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
T2KMH9	2.86e-99	4	776	29	746	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
A7LXU3	1.70e-91	19	777	43	768	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004719_01343.