CAZyme Information: MGYG000004721_00420

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000004721_00420
• CAZy Family: PL11
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1509	MGYG000004721_6|CGC2	162466.99	5.1742

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004721	2389811	MAG	Denmark	Europe

• Gene Location: Start: 25388; End: 29917 Strand: +

Full Sequence      

MKKIVTTVMAFLLTMSAQAQRTVDKLDHGLVAVKTSGGVFCSWRINGEEYYDVAYNLYRD
GVKLNTEPLTVSNYTDVSGTVSSKYTVKAVVRGQEQTTASAAVTPLTNNYKEIKIAEVPS
NIDGRNISGDYEPNDAIVADVDGDGEMEILLKLRNTKDAANSYPVEGKDFDIIQIYKQNG
KLLWWIDCGPNMVDFQSNEINIAAYDWDQDGKAECVLRGADGMVIHMADGTTKTIGDASV
NTRGDLVRSSAMTFTHTGAEYLLYLNGETGKPYSVSEYPLKRLESGETNLKKAWGDEYGH
RSSKNFFGAPYLDGRKPSIFLARGIYTRHKMIAYDVDPATHELKERWRWVNNTPGSPWYG
QGYHNYTIADVDWDGRDEIVFGSMVIDDNGHGLSTTGLGHGDAHHVGDFNPYVHGQEIAA
CNEDLPSNNYRDATTSKIYYRLAGGSDDGRSMAGNFTDDVIGGQFYSGHESSLIGCVSNK
STGLPTNNVTLNFRIYWDGDLLDETFNGMEVRNSAGVIYKYGKGKIETFTGVLTNNDTKA
TPCFQGDIFGDWREEVIMRSADNKSIRIYTTTIPTEHRNYTLLHDPQYRNAMITQMNGYN
PTPHVSYFLGEKEGITMAPPAPIMTDKNEVANGGSITSATNDKQVILAETGDATVNVADG
ASPYIFFDNAPTWVQGHDDNDNITTTTYKHTLTGGAFAGEMRLVKLGDGELTLPTVEQKY
TGNTEVWAGTVNFNGTMTGSHVWLNRFAVLNSDGGKFPKGIEADYGATIRPGGASKVGRI
ETNELKLNFGSIVEFDIDANGNADQLVADAMKIEKKDWTDGPEYLAPRFNFILPESLVAG
TYQLAKVGKIDGDLKDIVLTGMKGHKASLKYENGTISLVVEDMRAATSVVWTAEEDNTWQ
LAEKSNFKKQADNAADVFVSGDHVIFNDEAKNTTVQLTDDVAPATVEFDNSNAEYTLTGS
HSLIDNVDIVKNGDGNVNITNENTFKGSVTVNKGSLTVNTLGQVDGVNTGALGLYTNKVT
LNGGTLIPAKTMTTAHPVALGSNGGTINTPSNVTMTMTGSISGTGNVLTKEGDGTLVLAP
SASYSKLIVNGGTVKSSEPGSTRHAYPTTIVLNDATLRDADGIGTESSSSHTANVEVPEG
KSYWYLDSRCNYSGTLKGAGTLNVGVTSVRSYMKGNWSQFTGTVNFNASKTGQYTPHLVW
DNTYGLKNATVGVYCEVSNNDKSVTLGNLVGNGQLFGKGTWTIGSLNEDVKYTGKIDGGK
IVKTGDGLWTMSSLPTNLSGASSVNGGVLNLSNSAKKEQFFGSYSTTVSGTGTLAGQALL
YGITVNAGGTLKPGNYTSSIPTGSLKTTSGVFCNVGSKAKFFLYNNKNGATSRSYLEVGG
TFQLNGDLVVEAYSKYAPKAGDSFTLWTANNFMGTPSSVTLPKLPSNLAWDTSDLYKTTG
VLKVVAATGISQLGVNDEFTGSVYTVGGVKVGELTTTKATALKDLKTMGVASGIYFIRVA
GETLKYTVK

Enzyme Prediction     

No EC number prediction in MGYG000004721_00420.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	20	635	1.2e-161	0.9785478547854786

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	1.46e-170	23	607	1	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
pfam18370	RGI_lyase	3.81e-35	21	105	1	85	Rhamnogalacturonan I lyases beta-sheet domain. This is the beta-sheet domain found in rhamnogalacturonan (RG) lyases, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 carrying this domain, such as YesW (EC:4.2.2.23) and YesX (EC:4.2.2.24), cleave glycoside bonds between rhamnose and galacturonic acid residues in RG-I through a beta-elimination reaction. Other family members carrying this domain are hemagglutinin A, lysine gingipain (Kgp) and Chitinase C (EC:3.2.1.14).
PRK15319	PRK15319	3.05e-07	956	1417	672	1124	fibronectin-binding autotransporter adhesin ShdA.
PRK15319	PRK15319	2.44e-04	944	1330	734	1071	fibronectin-binding autotransporter adhesin ShdA.
PRK15319	PRK15319	2.84e-04	697	1465	999	1649	fibronectin-binding autotransporter adhesin ShdA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIM09959.1	0.0	16	1502	7	1531
AGB29439.1	0.0	6	1505	8	1515
BCS86257.1	0.0	24	897	3	883
ADU75030.1	3.83e-126	21	610	257	805
ALX08998.1	3.83e-126	21	610	257	805

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z8R_A	1.28e-123	19	611	1	570	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
4CAG_A	1.16e-116	15	611	3	577	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2ZUY_A	2.61e-112	20	610	5	590	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	1.67e-122	19	611	38	607	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	1.13e-111	20	610	5	590	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000269	0.999014	0.000220	0.000164	0.000161	0.000145

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004721_00420.