CAZyme Information: MGYG000004732_01423

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger
• CAZyme ID: MGYG000004732_01423
• CAZy Family: GH13
• CAZyme Description: Pullulanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
644	MGYG000004732_20|CGC2	70835.62	5.3519

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004732	2775580	MAG	China	Asia

• Gene Location: Start: 29348; End: 31282 Strand: +

Full Sequence      

MNLTDWPVYTGNDLGAVYTKRATTFTLWAPAAASVTLHRYTTGTDAEPGARELDPVALHP
GAQGTWRATVGGDLAGQYYVYELTFADGHTAMAVDPYARACGCNGQRGMVLDLQAAEPEN
WAADRRPAAANDPAARSVWEVHVADFSADEHSGVRPEWRGKFLAFTQPDTTLDCQGEYPT
CLNYLRHLGVSHVQLQPIFDFATVDESAGDKGGYNWGYDPLNYNVPEGSYATDPFHGEVR
VRECRAMIAALHKAGMGVVMDVVYNHTYFSDNWMERITPGYWLRRTADGQLTNGSGCGCD
LASEHPMVRKYIVDSVVYWATAYHVDGFRFDLMALHDVETMNAIRAALDKLPGGKNILMY
GEPWQGGATRMEEGAVPADKNALPQLDGRIGIFCDNTRDTVKGGVFDARSPGYVNGAPGA
GYTLLHAVGAFRDGADGMRPQNPGQIVQYVSAHDNFTLWDKLKAVAGRHTYDIFDGDLLA
QNRMCAAICLTCQGMPFWQAGEEFARTKHGDPNTYKGPLKENALDWSRAARMGELVEFYQ
GLLAIRREWPELTGLTDAAQPAPLLLAMPGWLIGFVPERADNAPNRLVVYYNPERTAQWV
DLPGGRWRTLCDGTRAQARPFGPEHAGRMELPPVSVVILAAQKD

Enzyme Prediction     

EC	3.2.1.41	3.2.1.68

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	182	503	4.3e-104	0.9965397923875432

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd11341	AmyAc_Pullulanase_LD-like	0.0	138	546	5	406	Alpha amylase catalytic domain found in Pullulanase (also called dextrinase; alpha-dextrin endo-1,6-alpha glucosidase), limit dextrinase, and related proteins. Pullulanase is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. Pullulanases are very similar to limit dextrinases, although they differ in their action on glycogen and the rate of hydrolysis of limit dextrins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
TIGR02104	pulA_typeI	0.0	5	620	3	605	pullulanase, type I. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family consists of pullulanases related to the subfamilies described in TIGR02102 and TIGR02103 but having a different domain architecture with shorter sequences. Members are called type I pullulanases.
COG1523	PulA	1.03e-123	24	642	70	692	Pullulanase/glycogen debranching enzyme [Carbohydrate transport and metabolism].
TIGR02103	pullul_strch	3.92e-110	10	527	124	729	alpha-1,6-glucosidases, pullulanase-type. Members of this protein family include secreted (or membrane-anchored) pullulanases of Gram-negative bacteria and pullulanase-type starch debranching enzymes of plants. Both enzymes hydrolyze alpha-1,6 glycosidic linkages. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family is closely homologous to, but architecturally different from, the Gram-positive pullulanases of Gram-positive bacteria (TIGR02102). [Energy metabolism, Biosynthesis and degradation of polysaccharides]
PLN02877	PLN02877	1.88e-97	9	526	211	791	alpha-amylase/limit dextrinase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADD61743.1	2.45e-313	7	642	10	640
ADD61669.1	4.50e-307	7	643	10	642
ATL90051.1	3.64e-220	14	613	28	621
QIA41780.1	4.18e-219	14	613	28	621
ATO99834.1	4.18e-219	14	613	28	621

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LSA_A	1.23e-152	9	638	59	710	ChainA, Pullulanase [Ruminococcus bromii]
7LSR_A	1.93e-151	9	638	59	710	ChainA, Pullulanase [Ruminococcus bromii]
7LSU_A	2.04e-151	9	638	59	710	ChainA, Pullulanase [Ruminococcus bromii]
7LST_A	2.10e-151	9	638	59	710	ChainA, Pullulanase [Ruminococcus bromii]
2WAN_A	1.66e-136	9	639	313	919	Pullulanasefrom Bacillus acidopullulyticus [Bacillus acidopullulyticus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O33840	1.62e-110	1	639	213	840	Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2
C0SPA0	8.12e-102	5	620	97	691	Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1
Q8GTR4	5.50e-62	14	527	208	791	Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2
A0A0H2ZL64	4.81e-60	26	522	450	949	Pullulanase A OS=Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) OX=373153 GN=spuA PE=3 SV=1
A0A0H2UNG0	6.68e-60	26	522	465	964	Pullulanase A OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=spuA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000051	0.000002	0.000001	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004732_01423.