CAZyme Information: MGYG000004739_00094

Basic Information

• Species: Actinomyces sp000220835
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; Actinomyces sp000220835
• CAZyme ID: MGYG000004739_00094
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
737	MGYG000004739_2|CGC1	81964.41	4.6174

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004739	2692200	MAG	China	Asia

• Gene Location: Start: 39263; End: 41476 Strand: -

Full Sequence      

MTDVATPEPARTPVHVDPWILADVAYARYHNPHEVLGAHVGEDGVTIRTVRHLADAVAVV
TAGGVFPAEHEQDGVWVAVVPGQTVPDYRIEVTYGEETTTVDDPYRFLPTLGEMDTYLIS
EGRHEGLWEVLGAHLKHYSGPMGEVEGVAFAVWAPNARAVRVVGDFNYWDGTASAMRSLG
ASGVWELFVPGVGVGARYKFELCFSDGSWHQKADPLARATEVPPATASVVTDRFHEWGDA
DWMAKRAITDPHSGPMSIYEVHVGSWRQGLGYRGLADELVPYVKEAGFTHVEFLPVAEHP
FGGSWGYQITGYYAPTARFGTPDDFRYLVDQLHQAGIGVILDWVPAHFPKDDWALARFDG
TALYEDPDPQRGEHPDWGTYVFNFGRNEVRNFLVANALYWMGEFHADGLRVDAVASMLYL
DYSREEGQWHPNQFGGRENLEAISFLQEATATAYRKNPGTVMIAEESTAWPGVTAPTEYG
GLGFGLKWNMGWMNDTLRYLAEEPINRRYHHGELTFSLVYAFSEQFVLPMSHDEVVHGKG
SLLSKMPGDPWQELAGLRALYAYQWSHPGKQLLFMGQEFGQGAEWNAENSLDWWILDDPG
HQELLKLVSDLNRFYTESPALWADDFSHRGFEWIEAGDGDHNVLSYLRKGTGPDGRADLM
VAVINFAGTPHEGYRVGLPFAGGWDEVINTDAPEYGGSGVVNLGHVEAEELPWNGRPASV
RLRVPPLGAVFLRPSQD

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	281	581	2e-162	0.9966777408637874
CBM48	131	221	5.3e-18	0.9078947368421053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	0.0	16	737	1	726	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	1	735	488	1223	glycogen branching enzyme; Provisional
PRK12313	PRK12313	0.0	109	736	5	629	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	130	737	26	624	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	106	734	1	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CED91062.1	0.0	1	737	1	750
QQM66893.1	0.0	1	737	1	737
VEG30063.1	0.0	1	737	1	743
VEG54793.1	0.0	1	737	1	737
ALC99599.1	0.0	9	737	19	748

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3K1D_A	1.65e-316	17	735	6	722	Crystalstructure of glycogen branching enzyme synonym: 1,4-alpha-D-glucan:1,4-alpha-D-GLUCAN 6-glucosyl-transferase from mycobacterium tuberculosis H37RV [Mycobacterium tuberculosis H37Rv]
5GQW_A	1.07e-252	15	733	20	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	1.51e-252	15	733	20	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR1_A	1.51e-252	15	733	20	772	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR0_A	2.14e-252	15	733	20	772	Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q5Z0W8	0.0	24	732	35	739	1,4-alpha-glucan branching enzyme GlgB OS=Nocardia farcinica (strain IFM 10152) OX=247156 GN=glgB PE=3 SV=1
Q2J6Q9	0.0	29	735	104	812	1,4-alpha-glucan branching enzyme GlgB OS=Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3) OX=106370 GN=glgB PE=3 SV=1
Q47SE7	0.0	29	732	42	744	1,4-alpha-glucan branching enzyme GlgB OS=Thermobifida fusca (strain YX) OX=269800 GN=glgB PE=3 SV=1
Q8G5L0	0.0	27	734	14	735	1,4-alpha-glucan branching enzyme GlgB OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=glgB PE=3 SV=1
Q0SGR9	0.0	29	732	24	728	1,4-alpha-glucan branching enzyme GlgB OS=Rhodococcus jostii (strain RHA1) OX=101510 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000045	0.000010	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004739_00094.