CAZyme Information: MGYG000004748_03466

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000004748_03466
• CAZy Family: CBM50
• CAZyme Description: Integration host factor subunit alpha

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
396	MGYG000004748_31|CGC1	43860.38	4.3485

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004748	4696076	MAG	China	Asia

• Gene Location: Start: 12144; End: 13334 Strand: +

Full Sequence      

MNEKLNIQDLVDMLAEKHGMSKKNADSFVKEFFQLIEEALEKDKYVKVKGLGTFKLIDVE
SRESVNVNTGERFEIQGHTKVSFAPEPTLKDIINKPFAHFETVVLNDNTVLEDTPVDSEN
DDDDEQETKASEQQETETNEQSVENIEAVVKAPAEIIAEVPDIPSAASEEKAEAIEEMPV
IPVQKEEIEEIIEEKVGEEVPAAATTDSSTMKYFIGIVVFVVVLCVGAVAYLYSPDWFEQ
FTSKPPTEEIVDAKADKPADKTIITDSIVTKDTVQVARVDTVREALANSSKSDKASKETP
ENTVREQAVSASIASKEQKKTVATPFKPDSVGYTIVGTEATYTIQEGETLTRVALRFYGT
KALWPYIVKHNPGVIKNPDNVPYGTTIKIPKLVKKQ

Enzyme Prediction     

No EC number prediction in MGYG000004748_03466.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00216	Bac_DNA_binding	1.80e-19	6	93	1	88	Bacterial DNA-binding protein.
cd13835	IHF_A	1.22e-16	8	93	3	88	Alpha subunit of integration host factor (IHFA). This subfamily consists of the alpha subunit of integration host factor (IHF) and IHF-like domains. IHF is a nucleoid-associated protein (NAP) that binds and sharply bends many DNA targets in a sequence specific manner. It is a heterodimeric protein composed of two highly homologous subunits IHFA (IHF-alpha) and IHFB (IHF-beta). It is known to act as a transcription factor at many gene regulatory regions in E. coli. IHF is an essential cofactor in phage lambda site-specific recombination, having an architectural role during assembly of specialized nucleoprotein structures (snups). IHF is also involved in formation as well as maintenance of bacterial biofilms since it is found in complex with extracellular DNA (eDNA) within the extracellular polymeric substances (EPS) matrix of many biofilms.
COG0776	HimA	3.72e-16	4	96	1	93	Bacterial nucleoid DNA-binding protein [Replication, recombination and repair].
cd13832	IHF	7.83e-15	9	91	3	85	Integration host factor (IHF) and similar proteins. This subfamily includes integration host factor (IHF) and IHF-like domains. IHF is a nucleoid-associated protein (NAP) that binds and sharply bends many DNA targets in a sequence specific manner. It is a heterodimeric protein composed of two highly homologous subunits IHFA (IHF-alpha) and IHFB (IHF-beta). It is known to act as a transcription factor at many gene regulatory regions in E. coli. IHF is an essential cofactor in phage lambda site-specific recombination, having an architectural role during assembly of specialized nucleoprotein structures (snups). IHF is also involved in formation as well as maintenance of bacterial biofilms since it is found in complex with extracellular DNA (eDNA) within the extracellular polymeric substances (EPS) matrix of many biofilms. This subfamily also includes the protein Hbb from tick-borne spirochete Borrelia burgdorferi, responsible for causing Lyme disease in humans. Hbb, a homodimer, shows DNA sequence preferences that are related, yet distinct from those of IHF.
cd13831	HU	1.99e-14	6	91	1	86	histone-like DNA-binding protein HU. This subfamily includes HU and HU-like domains. HU is a conserved nucleoid-associated protein (NAP) which binds non-specifically to duplex DNA with a particular preference for targeting nicked and bent DNA. It is highly basic and contributes to chromosomal compaction and maintenance of negative supercoiling, thus often referred to as histone-like protein. HU can induce DNA bends, condense DNA in a fiber and also interact with single stranded DNA. It contains two homologous subunits, alpha and beta, typically forming homodimers (alpha-alpha and beta-beta), except in E. coli and other enterobacteria, which form heterodimers (alpha-beta). In E. coli, HU binds uniformly to the chromosome, with a preference for damaged or distorted DNA structures and can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Anabaena HU (AHU) shows preference for A/T-rich region in the center of its DNA binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QPH57227.1	1.62e-125	1	396	1	393
QBJ16968.1	1.62e-125	1	396	1	393
QMI81676.1	1.62e-125	1	396	1	393
QUT47016.1	1.05e-124	1	396	1	397
QQA31617.1	2.63e-124	1	396	1	393

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1HUE_A	5.68e-10	5	93	1	89	ChainA, HU PROTEIN [Geobacillus stearothermophilus],1HUE_B Chain B, HU PROTEIN [Geobacillus stearothermophilus],1HUU_A Dna-Binding Protein Hu From Bacillus Stearothermophilus [Geobacillus stearothermophilus],1HUU_B Dna-Binding Protein Hu From Bacillus Stearothermophilus [Geobacillus stearothermophilus],1HUU_C Dna-Binding Protein Hu From Bacillus Stearothermophilus [Geobacillus stearothermophilus]
5J0N_I	2.30e-09	9	90	6	87	ChainI, Integration host factor subunit alpha [Escherichia coli],5J0N_K Chain K, Integration host factor subunit alpha [Escherichia coli]
1IHF_A	2.48e-09	9	90	7	88	INTEGRATIONHOST FACTOR/DNA COMPLEX [Escherichia coli],1OUZ_A Chain A, Integration Host Factor Alpha-subunit [Escherichia coli],1OWF_A Chain A, Integration Host Factor Alpha-subunit [Escherichia coli],1OWG_A Crystal structure of WT IHF complexed with an altered H' site (T44A) [Escherichia coli],2HT0_A IHF bound to doubly nicked DNA [Escherichia coli],5WFE_K Cas1-Cas2-IHF-DNA holo-complex [Escherichia coli S88]
5LVT_A	7.10e-09	6	93	3	90	Structureof HU protein from Lactococcus lactis [Lactococcus lactis subsp. lactis Il1403],5LVT_B Structure of HU protein from Lactococcus lactis [Lactococcus lactis subsp. lactis Il1403],5LVT_C Structure of HU protein from Lactococcus lactis [Lactococcus lactis subsp. lactis Il1403],5LVT_D Structure of HU protein from Lactococcus lactis [Lactococcus lactis subsp. lactis Il1403]
5EKA_A	1.50e-08	9	95	10	96	HUDNA-binding protein from Thermus thermophilus [Thermus thermophilus HB8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2IJA7	1.99e-11	9	94	5	90	Integration host factor subunit alpha OS=Anaeromyxobacter dehalogenans (strain 2CP-C) OX=290397 GN=ihfA PE=3 SV=1
B4UAP1	1.99e-11	9	94	5	90	Integration host factor subunit alpha OS=Anaeromyxobacter sp. (strain K) OX=447217 GN=ihfA PE=3 SV=1
B8J836	1.99e-11	9	94	5	90	Integration host factor subunit alpha OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) OX=455488 GN=ihfA PE=3 SV=1
Q65TL4	2.55e-11	9	95	7	93	Integration host factor subunit alpha OS=Mannheimia succiniciproducens (strain MBEL55E) OX=221988 GN=ihfA PE=3 SV=1
A7HBJ3	4.90e-11	9	94	5	90	Integration host factor subunit alpha OS=Anaeromyxobacter sp. (strain Fw109-5) OX=404589 GN=ihfA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
213	235