CAZyme Information: MGYG000004759_00243

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Paraprevotella
• CAZyme ID: MGYG000004759_00243
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
856		95814.51	6.7562

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004759	2383712	MAG	China	Asia

• Gene Location: Start: 31111; End: 33681 Strand: +

Full Sequence      

MKQLYALLIAGLCTCQTAQAKDYIKYKDASVQISERVEDLMARMTLDEKVAQLMQMGSHM
IVHDGKVDLGKMKAICQKNGIGFFEGITLTSEEAHRIMSEVQHYMIDSTRLGIPVFTITE
SLHGSVNDGATIYPQAIALGSTFNPELVYQMTANISEELKVQGITQSLTPVVDVCRDLRW
GRVEECFGEDPFLVRTMGAHQVKGYMDQGISPMLKHFGAHGSPSGGLNLASVHCGQRDLL
SIYLQPFEYIVKTVKPWAVMSSYNSWNGVPNSASEYLMTTLLRNEWGFQGYVYSDWGSID
MLNYFHKTARNRAEAAYQAFHAGLDVEAGINCYRNLKGLVTSGLLNMEEIDRAVRRVLTA
KFEMGLFEYRFPTEKEFKKHLHTPKQVALARKLAEESIVLLQNNEKLLPLDINKLHSVAL
IGPNAYQVQFGDYTWSRSNKDGVTLLEAFRKRAGNKIRIEYAKGCDLHTDTTSHIPQAVN
MAKNCDLSIVVVGSASASLSRDYSNVTCGEGYDLSDMTLTGAQEELIKAIHATGKPVIVV
LLSGKPFAMPWVKENIPTILVQWYPGEQGGNALYDVLFGEVNPSGKLNYSFPKSTGNIPC
YYNHLPTDRGFYHQPGKPNQPGRDYVFDTPEPLWAFGHGLSYSEFEYCDMQTDKEEYDMD
DTIHIEVKLRNLSDIPGKEVVQVYVRDLISSVVTPIQELKAFSKVNISGKETKQVNLSIP
VHDLGLYNQAMKYVVEPGEFEIQVGGASDKIALQKKIGVTLHEEKKINLKKQENTTRKIK
KEKITVSGYVRDVQANPVENVRIESEGQSVSTDSDGFYQITTYSTATLLIKGKYIEPKKI
PVQGEANINIEIVRKK

Enzyme Prediction     

No EC number prediction in MGYG000004759_00243.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	108	327	2.3e-67	0.9722222222222222

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	2.90e-168	1	751	1	757	beta-glucosidase BglX.
COG1472	BglX	5.08e-90	44	434	1	372	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	9.28e-84	15	745	32	769	Probable beta-xylosidase; Provisional
pfam00933	Glyco_hydro_3	2.97e-70	45	360	1	316	Glycosyl hydrolase family 3 N terminal domain.
pfam01915	Glyco_hydro_3_C	2.98e-63	398	642	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AKA52531.1	0.0	1	855	1	859
QCT79413.1	0.0	1	855	1	859
ANQ61151.1	0.0	1	855	1	859
CAH08594.1	0.0	1	855	1	859
QUU03453.1	0.0	1	855	1	859

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5YOT_A	2.13e-175	25	751	6	748	Isoprimeverose-producingenzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]
7EAP_A	1.68e-174	25	751	6	748	ChainA, Fn3_like domain-containing protein [Aspergillus oryzae RIB40]
5Z87_A	1.32e-134	19	751	31	777	ChainA, EmGH1 [Aurantiacibacter marinus],5Z87_B Chain B, EmGH1 [Aurantiacibacter marinus]
4ZOA_A	1.00e-116	35	751	4	712	CrystalStructure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOA_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOB_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOB_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOD_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOD_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOE_A Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262],4ZOE_B Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262]
6R5I_A	5.68e-116	37	745	7	719	ChainA, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5I_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KMH0	4.67e-135	110	748	62	710	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
T2KMH9	1.64e-119	13	748	19	744	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
Q56078	5.05e-115	37	751	38	757	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	1.09e-112	1	751	1	757	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
P14002	1.39e-98	110	796	34	690	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001498	0.484889	0.512434	0.000538	0.000339	0.000288

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004759_00243.