CAZyme Information: MGYG000004763_01620

Basic Information

• Species: CAG-462 sp900291465
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; CAG-462; CAG-462 sp900291465
• CAZyme ID: MGYG000004763_01620
• CAZy Family: PL11
• CAZyme Description: Rhamnogalacturonan endolyase YesW

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
343	MGYG000004763_17|CGC2	37921.74	4.692

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004763	3504022	MAG	Denmark	Europe

• Gene Location: Start: 52645; End: 53676 Strand: -

Full Sequence      

MDRFFIGVAYLDGIPDPSTGVRTTNPSLIISRGIYKNWQVRALDLREDGLQTRWTFDTAE
HSDKWLAMCSHAFRVADLDGDGRDEVLYGSAAIDDNGNELWCTGNGHGDCLSVGKFVKDR
EGLQIVASFEEPGNYAGQGHGYACQVIDARDGSLIAGHGAGDHGDVGRSIVADIDPDSPN
FEYWSSINSGLYNCGGPSDAPVTADLPKGIGGGTMYNAAIYWSGELTRDLWDRGLIMSYR
ANPLTKRNRLVNFSTGYGSNQGNHSTKYNPCYYGDFLGDWREEVILGSEDNKSILVFSTN
YPTHHRFTHLMEDHTYDLSQAMQNMGYNQQTNLGFYLGADMNR

Enzyme Prediction     

No EC number prediction in MGYG000004763_01620.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	1	342	1.1e-100	0.5317324185248714

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	1.45e-80	1	335	262	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU56547.1	3.36e-187	1	340	346	683
QQR18606.1	3.36e-187	1	340	346	683
QDM11717.1	7.26e-186	1	340	348	687
QUT27383.1	7.49e-186	1	340	349	688
ALJ49009.1	7.49e-186	1	340	349	688

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CAG_A	2.24e-57	1	341	272	579	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2Z8R_A	4.11e-56	1	341	265	572	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
2ZUY_A	1.29e-51	1	343	269	595	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	3.58e-55	1	341	302	609	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	6.30e-51	1	343	269	595	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000028	0.000032	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004763_01620.