CAZyme Information: MGYG000004763_02660

Basic Information

• Species: CAG-462 sp900291465
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; CAG-462; CAG-462 sp900291465
• CAZyme ID: MGYG000004763_02660
• CAZy Family: GH31
• CAZyme Description: Alpha-xylosidase BoGH31A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
987	MGYG000004763_56|CGC1	113340.61	5.9328

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004763	3504022	MAG	Denmark	Europe

• Gene Location: Start: 6107; End: 9070 Strand: -

Full Sequence      

MSYKHLLLTVGLLLGVCSMTRADDYVKQGNTVTVTVSSPQDKGARKVRLQVIGDKIVRVE
ATPEADFPVKQSLMVVSQPDFNDFQVTETEDAVQVKTHAVCATVSKTDGRVVFRDGKGHL
LLAETARGGKVFTPFRVPEREIGVGTLTEQQRNAWSWQMKFDSPVDEAFYGLGQHQSGEL
NMKGKNEDLFQYNTKVSVPFVVSNRGYGLLWDSYSYCRFGNPEDYLQLNRAFTLYDKNGQ
EGALTGTYTDKAGRTLERREDSLYFEYACPETSELCRKYDRGGLANLPQGFQLDGAKVVY
EGSLLAEGDSPSVTFHFILYYAGYIKVFVDGKEVVPERWRTAWNPNAWKFACRLRTGVRI
PVRIEWKPDGGVSYCGLRVAQPRSEEEQGKLSVWSEMSRDMDYYFIAGDDLDEVIAGYRT
LTGKAQVMPRWVMGFWQSRERYKTSDEILRTLAEFRRRHIPVDNIVQDWNYWPEDAWGSH
EFESVRYPDPQAMLDSVHALHGRFMISVWPKFYCSTKNYQELDAHGWMYRQAVTDSIKDW
VGPGYAGSFYDAYAEGARKMFWRQMDENLYSKFQQGIDAWWMDASEPNVRDCTPMWYRKA
LCGPTALGSSTEYFNAYSIVNADAIYNGQRSVNPDRRVFLLTRSGFAGEQRYGTATWSGD
IATRWEDMRAQMTAGMNFSLAGIPFWGMDQGGFCVEKRYVAAQKLFDSTGQENEDLKEWR
ELQTRWHQFGCFVPLFRTHGQWPTREVWNIAPEDHPAYQSIVWYDRLRYRLMPYLYSLAG
AVHFRDYTMMRALVMDFNGDANVYDIKDQWMFGPSLMACPVGYYQARSRRVYLPEQCGWY
DFYTGRYHAGGQTITAEAPYERIPVYVPAGSILPFGPEMEWSDEKPAELINLYVYTGRDA
SFTLYEDENTNYGYERGHYATIRFSYNEATGELTIADRKGSFDGMLQRRRFNVVVVSPAK
AQALDLDAPQGTMVDYDGKAVTLKIVK

Enzyme Prediction     

EC	3.2.1.177

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	403	873	5.3e-138	0.9976580796252927

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam01055	Glyco_hydro_31	8.34e-170	404	873	1	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd06591	GH31_xylosidase_XylS	1.26e-151	423	768	1	322	xylosidase XylS-like. XylS is a glycosyl hydrolase family 31 (GH31) alpha-xylosidase found in prokaryotes, eukaryotes, and archaea, that catalyzes the release of alpha-xylose from the non-reducing terminal side of the alpha-xyloside substrate. XylS has been characterized in Sulfolobus solfataricus where it hydrolyzes isoprimeverose, the p-nitrophenyl-beta derivative of isoprimeverose, and xyloglucan oligosaccharides, and has transxylosidic activity. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. The XylS family corresponds to subgroup 3 in the Ernst et al classification of GH31 enzymes.
COG1501	YicI	8.67e-147	386	957	220	755	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
cd06603	GH31_GANC_GANAB_alpha	6.58e-61	423	874	1	427	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
cd06598	GH31_transferase_CtsZ	5.82e-59	423	779	1	330	CtsZ (cyclic tetrasaccharide-synthesizing enzyme Z)-like. CtsZ is a bacterial 6-alpha-glucosyltransferase, first identified in Arthrobacter globiformis, that produces cyclic tetrasaccharides together with a closely related enzyme CtsY. CtsZ and CtsY both have a glycosyl hydrolase family 31 (GH31) catalytic domain; CtsY belongs to a different subfamily. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNT65144.1	0.0	4	985	5	983
BCS86029.1	0.0	1	985	1	981
VEH16024.1	0.0	14	985	12	980
ADE81187.1	0.0	29	985	26	1007
QVJ80354.1	0.0	29	985	26	1030

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JOU_A	0.0	25	985	27	955	Bacteroidesovatus Xyloglucan PUL GH31 [Bacteroides ovatus],5JOV_A Bacteroides ovatus Xyloglucan PUL GH31 with bound 5FIdoF [Bacteroides ovatus]
7KMP_A	3.95e-295	22	983	57	975	ChainA, Alpha-xylosidase [Xanthomonas citri pv. citri str. 306],7KNC_A Chain A, Alpha-xylosidase [Xanthomonas citri pv. citri str. 306]
2XVG_A	1.14e-283	45	984	66	985	crystalstructure of alpha-xylosidase (GH31) from Cellvibrio japonicus [Cellvibrio japonicus],2XVK_A crystal structure of alpha-xylosidase (GH31) from Cellvibrio japonicus in complex with 5-fluoro-alpha-D-xylopyranosyl fluoride [Cellvibrio japonicus],2XVL_A crystal structure of alpha-xylosidase (GH31) from Cellvibrio japonicus in complex with Pentaerythritol propoxylate (5 4 PO OH) [Cellvibrio japonicus]
6JR6_A	3.59e-63	401	944	236	762	Flavobacteriumjohnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR7_A Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101]
6JR8_A	4.07e-62	401	944	236	762	Flavobacteriumjohnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXT0	0.0	11	985	15	954	Alpha-xylosidase BoGH31A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02646 PE=1 SV=1
Q9P999	8.02e-129	388	984	177	729	Alpha-xylosidase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=xylS PE=1 SV=1
Q9F234	1.38e-57	401	928	230	732	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
Q01336	7.15e-50	392	740	204	523	Uncharacterized family 31 glucosidase ORF2 (Fragment) OS=Pseudescherichia vulneris OX=566 PE=3 SV=1
B3PEE6	4.44e-47	403	967	234	773	Oligosaccharide 4-alpha-D-glucosyltransferase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=agd31B PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000724	0.398931	0.599653	0.000257	0.000232	0.000196

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004763_02660.