CAZyme Information: MGYG000004769_00819

Basic Information

• Species: Phascolarctobacterium faecium
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium; Phascolarctobacterium faecium
• CAZyme ID: MGYG000004769_00819
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
516	MGYG000004769_11|CGC3	59628.68	8.9751

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004769	2442638	Isolate	United States	North America

• Gene Location: Start: 566400; End: 567950 Strand: +

Full Sequence      

MNRIEKGSLLVLAIIVAFNIFFGLGSIPLLDPDEPVYAETAREMIQFNDYLSPRIYNEYW
FDKPPMYYWLVAGAMHVFGDGEFAARFPAALMAFLTVIMLYYSITKLFNERAGFWSALVL
ATSVQFFYLGKAAVTDTTLLFFLTGSLLCYLHKQYWLMYVCMALATVTKGPIGIVFPGAI
IFLHILCTGQWQKLFKMHCLRGLLLYFFIAAPWYYLMYQVHGMEFINTFLGFHNLTRFTT
PEHPTRVLWWYYFPVIILGLFPWTGLLLQSIKASITDSRSDDLEKMLFVNIWWLFVLIFF
TISQTKLVSYILPMFPALAIMIGWNIARMQKENYGIRWSWIFGSGIMFLLLTAGWVIGGQ
QLPEVAFGGIVLGSVTFILGVSIVWALWRYRDINFSAWLHVVTGVLTMVIAFSFLLPMVA
DRFSVKEIVRVYQTKCDQQKPVYVDKFLRPGFMYYTGVPGEEVKPKSGDLARVLNDPGQK
YLLVRGLELRRLKDKDKLQNLNEIKEIGDIYLLEKY

Enzyme Prediction     

No EC number prediction in MGYG000004769_00819.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	4	441	3.2e-121	0.8351851851851851

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1807	ArnT	2.54e-62	3	431	4	446	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
PRK13279	arnT	1.49e-35	1	387	2	397	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
pfam13231	PMT_2	2.36e-20	62	215	1	159	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	6.66e-08	59	226	58	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
COG1287	Stt3	0.003	62	143	86	172	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV77242.1	7.26e-252	1	515	1	518
QNH53437.1	1.99e-178	10	492	13	502
AOH48432.1	2.81e-178	10	506	13	516
ANR70824.1	1.71e-174	10	492	13	502
AKT54119.1	2.57e-171	10	508	13	507

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	3.18e-37	11	335	35	373	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O67601	4.40e-36	6	351	2	327	Uncharacterized protein aq_1704 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1704 PE=3 SV=1
C5BDQ8	3.51e-32	29	377	27	391	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Edwardsiella ictaluri (strain 93-146) OX=634503 GN=arnT PE=3 SV=1
O67270	1.77e-31	5	340	3	338	Uncharacterized protein aq_1220 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1220 PE=3 SV=1
Q7N3Q9	1.01e-30	2	321	4	331	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01) OX=243265 GN=arnT PE=2 SV=1
A0KGY4	1.46e-29	28	333	24	337	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000012	0.000006	0.000001	0.000000	0.000000	0.000002

TMHMM  Annotations     

start	end
7	29
83	105
112	134
164	186
199	221
248	267
287	304
308	327
334	356
366	388
395	417