CAZyme Information: MGYG000004792_00296

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus
• CAZyme ID: MGYG000004792_00296
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
737	MGYG000004792_2|CGC4	80776.21	4.894

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004792	1933522	MAG	China	Asia

• Gene Location: Start: 94752; End: 96965 Strand: +

Full Sequence      

MIMVTIDLDFVEGLTLEERAALVSGKDFWFTAKVPGVDRMMMSDGPSGLRKQNIDASPAD
INDSVDAVCFPSSALTASSFDDDMLYKLGTHLGIAANAEHLGVLLGPGVNIKRSPLAGRN
FEYFSEDPLLAGRMGVAYVKGVQDQGVGVSVKHFAANNRENQRFTSSSNIDERALREIYL
AQFERIVKQAHPATLMCSYNRLNGTLNSQNQRLLTDILRNEWGFQGLVMSDWGAVADQVA
AIKAGLDLEMPGKGQASVDEIVAAVKDGRLQESTLNRSALRVLRMVEKWGKKVTKADTYD
KKEQHEFARQMADDSIVLLKNEQNQLPLNATDSLAIIGELAAKPRFEGGGSSHVNAHQVV
TPLDAAPAGAQYAQGYSLKETAADEQLAAAAVELAKKSDQVVFFAGFPEEMETEGFDKDT
ISLPTNQNDLLAKVAAANSHVTVVLENGSVLEMPWADQVPAIVETYLAGEAVGEATWDIL
TGKVNPSGKLSESFPLRLQDNPTYGTFGKDHDEEDYHEGIFVGYRYYDLHEMPVRFPFGH
GLSYTSFAYNNLTVEEGTDTVKVGFDLTNTGKVAGKEVAQVYVANQVSRVEIPVKELRDF
TKVALEAGESKHVTLTLPRQAFAYYNVAKQQWEADNGQYKIMVGSSSRDIRLSMAFTLTI
GTNPTNEVTGNTYVGDLWANQDPHVQKALKDSGLYEILAPMMKDEKWVAISANIPLRSLS
AMGVDEKIRKAFFKALK

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.45

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	36	251	1.3e-63	0.9629629629629629

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.50e-87	70	658	120	764	beta-glucosidase BglX.
PLN03080	PLN03080	9.72e-60	67	617	111	742	Probable beta-xylosidase; Provisional
COG1472	BglX	6.64e-59	67	375	80	394	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam01915	Glyco_hydro_3_C	4.16e-58	316	524	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	1.67e-34	70	285	88	316	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QZN93572.1	0.0	3	737	1	735
AMS07885.1	0.0	3	737	1	735
AMS08576.1	0.0	3	734	1	730
AJT51049.1	0.0	3	725	1	720
QLI93846.1	0.0	3	725	1	720

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	4.33e-213	11	719	9	731	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	1.85e-185	11	658	9	663	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
3AC0_A	3.32e-120	14	650	13	828	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]
3ABZ_A	4.20e-116	14	650	13	828	Crystalstructure of Se-Met labeled Beta-glucosidase from Kluyveromyces marxianus [Kluyveromyces marxianus],3ABZ_B Crystal structure of Se-Met labeled Beta-glucosidase from Kluyveromyces marxianus [Kluyveromyces marxianus],3ABZ_C Crystal structure of Se-Met labeled Beta-glucosidase from Kluyveromyces marxianus [Kluyveromyces marxianus],3ABZ_D Crystal structure of Se-Met labeled Beta-glucosidase from Kluyveromyces marxianus [Kluyveromyces marxianus]
2X40_A	2.54e-113	9	658	6	713	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	2.37e-212	11	719	9	731	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
F6C6C1	8.67e-182	11	669	9	682	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
E7CY69	3.46e-178	11	669	9	682	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
Q5BFG8	1.40e-128	14	650	18	830	Beta-glucosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglB PE=1 SV=1
P27034	4.46e-126	9	660	4	812	Beta-glucosidase OS=Rhizobium radiobacter OX=358 GN=cbg-1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999868	0.000175	0.000013	0.000000	0.000000	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004792_00296.