CAZyme Information: MGYG000004834_00780

Basic Information

• Species: Massiliomicrobiota sp002160865
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Massiliomicrobiota; Massiliomicrobiota sp002160865
• CAZyme ID: MGYG000004834_00780
• CAZy Family: GH29
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1158		127097.15	4.1762

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004834	2366163	MAG	China	Asia

• Gene Location: Start: 1780; End: 5256 Strand: -

Full Sequence      

MNKSKILKGALAGTLAMTIVGSAVYSSNALVYDTDLEDKTVTEPESWGALPNEAQLQYHD
DELAAFIHFGVNTFTDKEWGDGTESPSVFNPTGDFGESAKSMVEALKNAGFKKVIVTAKH
HDGFCIWPSEYTEHDIENSPYKDGKGDILAEISAACTEYDMDMGLYLSPWDENAESYGYY
DKDGNPLLDENGNPIDGMTMEEVKEKDALDYNDYYVNQITEIVTNPKYGNSGKFTEWWMD
GAKGEGGDAQEYDTERILKTIKDNEPGILIFGGGTEGGIHWIGNEKGQASDTTWAKVDSE
KGDGYSEADGFTYGIPDGDHWSVPESDVSITSGWFYHDGDLPKSISQLGNIYFQTVGNGS
PLLLNFPPNQTGDLDGSIYSRLNEFKNAIDNTFKTNLLAQNSKAGASSVYENAKEYSPVK
AIDGDEDTYWTTEKGEITGTLEIEFDEVKEFDVVSIEEYIEKGQRISKFEVEYQTADGQW
LPFEDGHTIGAKKLLRIDPVQAKAIRIHISDSCAEPMISEVGIYKTADGFEKTQPLPEAY
TKFVPGDEGLELNKGSWDDDHQGNTGWEQQEDGSYTTTDGWANIKYTFEGTYFALVGTKG
PDYGSFDIYIDGKYTSSANASAGEITPGQILYESPTLGAGTHTIQLTTGTNRKPVNFAYG
YYLGTNENGLFEIETDSYQVTEGDSIKVKIKRNGGSTGEVTVNVTTPPGTAVQGQYYENI
TNETITFKDGEKVKYVTLKTIDNLDVTGNKDFYVEIFSPTGGAQLGMRSTARVLIVENDK
RIADAVEQTSNISESWYKAESWDAFDQAYLKAKAAVDDDSMSDDEKMKLSNALMEAYNNL
EVISQYSVRNAMIMPDNFEDEKTLEVELGILDSKHENIVANEYLKMVNGNEYSNGRLINK
IKDGNTITIPLNVQKTGLYNFDFTYQATQPMSLIISGDHIVKQTISLDASETLIEKSIPL
NIQETGISQVVITAQSLAKSDGDLAIDNAKIKLQEVSNGKIESIAIEQMPSKTVYALGDT
LDLTGLKVIAKYDTGATTDITHKVEVTGFDSSKVGEQTLTVHYGEFETQFTVTVKGKSDE
INPGTDTPEKPGQDNPNQGNSGQTGEKPSTTPTVNGTTSSHDKVKTSDEQVIGVFVIGLL
GAACLFVVMKNKKSDSQD

Enzyme Prediction     

No EC number prediction in MGYG000004834_00780.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH29	43	387	8.1e-61	0.8872832369942196

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3669	AfuC	2.61e-76	44	524	4	430	Alpha-L-fucosidase [Carbohydrate transport and metabolism].
pfam01120	Alpha_L_fucos	6.97e-20	89	384	79	323	Alpha-L-fucosidase.
smart00812	Alpha_L_fucos	1.97e-19	58	385	23	326	Alpha-L-fucosidase. O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. Family 29 encompasses alpha-L-fucosidases, which is a lysosomal enzyme responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Deficiency of alpha-L-fucosidase results in the lysosomal storage disease fucosidosis.
pfam07523	Big_3	2.16e-13	1010	1074	2	67	Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
pfam03160	Calx-beta	2.74e-12	669	756	2	90	Calx-beta domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SLK22631.1	0.0	3	1153	4	1157
QBJ76368.1	0.0	3	1153	4	1157
ATD54107.1	0.0	3	1153	4	1157
PLT74914.1	1.53e-185	50	1058	65	1068
QHB23055.1	2.21e-179	40	927	55	943

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6TR3_A	9.63e-145	50	562	27	546	Ruminococcusgnavus GH29 fucosidase E1_10125 in complex with fucose [[Ruminococcus] gnavus E1]
6TR4_A	1.44e-143	50	562	27	546	Ruminococcusgnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1],6TR4_B Ruminococcus gnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1]
6ORG_A	8.10e-118	48	526	8	450	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORG_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6OR4_A	1.13e-116	48	526	8	450	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6OR4_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_A Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6ORF_A	1.17e-116	48	526	8	450	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORF_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8GW72	1.32e-91	50	524	37	477	Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2
Q7XUR3	6.04e-78	50	536	39	486	Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0560400 PE=3 SV=2
A0A401ETL2	8.62e-09	896	1151	1251	1512	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1
Q9VTJ4	3.74e-06	46	168	37	182	Putative alpha-L-fucosidase OS=Drosophila melanogaster OX=7227 GN=Fuca PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.002831	0.995941	0.000368	0.000321	0.000273	0.000230

TMHMM  Annotations     

start	end
1131	1148