CAZyme Information: MGYG000004845_02428

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UBA1381; UBA4716
• CAZyme ID: MGYG000004845_02428
• CAZy Family: GH30
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1642		179361.16	4.1601

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004845	2789282	MAG	Sweden	Europe

• Gene Location: Start: 1312; End: 6240 Strand: -

Full Sequence      

MKAKRILSAITALSLLPAIGAVTGVPEAKAASTSATTVRLNPADASPFNNGEFEGWGTSL
CWWANRVGYNDALTQQAAQAFFSETGLGLDIARYNIGGGDDQYHNHIERSDSKVPGVWES
FEYTNENKDVSVTYDITNDKNQLNVAKAALAANPNIYFEGFSNSPPYFMTNSGCSSGAED
ASSDNLRTDMYDDFAKYIAEATKLMKQQGINFQSYSPMNEPDTNYWGANSPKQEGCHYDP
GDSQSNMIVETRKALDAAGFNDVLVAGMDETSIDSSVKNLDRLTDEAKAALGRIDTHTYS
GSNRSGLKAKAVELQKDLWMSEVDGSWDGFGLADRIIADMNGMMPSAWVIWDIIDIHKDS
NFTDPNGNKTEADNSVSDTSSLWGVGMADHDNSELVLTNKYYAFGQFTKYINPGDTIIAS
SNSTLAAYNKESGDIKIVASNSSNSDRNYIFDLSAFTNIGSEVTEIRTSDSGEKWANITN
EATLADGKITTTLKARSVTTYIVNGRGPTDYAYISGGGNQLGLGDSITLTVLSNLSGADD
VKWSVSDNSIAEITPEGVLTAKSHGTVTVYATIGDFTTSRTFEIPLYKLSGTPSWNNSSS
EPKDSDDYTHVADGDFNTYFDGTQGGWVMYDYGTPYKPDEILLAARSGNKMQIRTVGGTV
QGSNDAISWTDLYKISSALPSDKYTVVTSDMLSNKNAYRYFRYTNNSDMTNIAEFIINGA
PSSDVAEGEPSVTDIDEISDNFESDSNIFGAAPGDLSSNGAQVYASGLERFSNVFAPIKT
TSEAQLSEPITLGKNQRFRFTFNMFAGWEEKGKDNTLDIKDADGNSIFSMTVNVESCSFT
KLMLGDTDLLNGGTLGAQCKTALSGRTGANGWDSTSQPYRNNVGYNKTIELLIDGQGSAS
LTISGGTAESTSYTAVIDKPVSIASLVVTGNHNSSAKRTVCYDNFDGDIITYAEDFDEPE
KPVASEPPILPESGELINLNFDNKTLESASSYGKAAGNPEFVTVDEKNCIQFDGTAATQV
KLTDANGNSLLTGVDEMTISFKMKPIDTAASWMFFAAPNDNEQSYKSEKYFGVLAENGSL
KIERYNNSGSRPEVASGAINMNEWNDVVIIVNSNDTSLYINGMLADTAASTVNISDMLGS
SSVAYLGKANWKQGEFATGYIDDFVIYNHSILSNAAIDIDTSNLTADIELPAEVNGNTVT
WTTSDESVITAEGKITRADETQSAVLTATVTVNGIEFKQEFTVTVLGFTSAIDKFTAYAD
GNTIKFTGGYSTDTPYNMYVAVYDSNERLVGTRTNTAEGSFEELPNGEYKVSCYIWNEEQ
KAMDDKVTKTVIIKSEFETDAYLFAHFVGNESDASHEQIYFSVSEDGQNWTTLNDQKPVL
TSTVGEQGVRDPYILRGEDGKFFIIATDLSIYNRQKRGEDAWSECQKSGSKSIVVWESTD
LVNWEEARLVEIAVNNAGCTWAPEAIYDPEKGMYMVFWASKISDDNFSNQRMYKSYTIDF
KTFTEPEIFIETDVSNIDTTIIEHNGIYYRFTKNESNSSVIMEKSRFLDGQWSAVEGYNL
GTMTGYEGPLIYKLNGEDKWCLLLDAYASRGGYKPFVTDDLESGVFTQGSDFTFDGTYRH
GTVMPITRAEYNRLIEAYGSAE

Enzyme Prediction     

No EC number prediction in MGYG000004845_02428.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	49	501	8e-136	0.9824561403508771
GH43	1386	1587	2e-65	0.9904306220095693
CBM62	589	715	1.3e-20	0.916030534351145

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08983	GH43_Bt3655-like	9.20e-102	1372	1638	1	262	Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 arabinofuranosidase Bt3655. This glycosyl hydrolase family 43 (GH43)-like family includes the characterized arabinofuranosidases (EC 3.2.1.55): Bacteroides thetaiotaomicron VPI-5482 (Bt3655;BT_3655) and Penicillium chrysogenum 31B Abf43B, as well as Bifidobacterium adolescentis ATCC 15703 beta-xylosidase (EC 3.2.1.37) BAD_1527. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
pfam14587	Glyco_hydr_30_2	9.56e-20	56	235	18	195	O-Glycosyl hydrolase family 30.
COG5520	XynC	2.01e-18	2	504	4	429	O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis].
cd08978	GH_F	4.43e-17	1390	1631	1	244	Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
pfam13385	Laminin_G_3	5.47e-12	1018	1177	5	150	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADU23821.1	3.18e-139	26	676	22	596
QTE67528.1	6.75e-130	37	503	31	510
AOZ97766.1	1.75e-123	37	504	60	582
CBK73885.1	6.71e-123	23	714	935	1634
ADL35667.1	2.88e-120	37	503	88	606

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CLW_A	3.25e-09	86	258	62	221	Crystalstructure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_B Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_C Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_D Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_E Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_F Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A3R0A696	7.62e-43	1070	1613	165	780	Alpha-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=blArafA PE=1 SV=1
A0A401ETL2	3.80e-33	7	459	8	570	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000349	0.998886	0.000180	0.000229	0.000189	0.000169

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004845_02428.