CAZyme Information: MGYG000004848_01129

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000004848_01129
• CAZy Family: GH43
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1380		157420.01	7.8992

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004848	2237652	MAG	China	Asia

• Gene Location: Start: 8751; End: 12893 Strand: +

Full Sequence      

MRKLFLSAIVMLTTAAPNLSYAKDIYVSTSFHEPADEGLRFIYSYDGLRWDTIQGTFLRP
EVGVQKVMRDPSIVKGPDGTFHLVWTSSWKGDKGFGYASSKDLIHWSTPRLINVMDDPTT
VNVWAPELFYDDVKKQYMIVWASCIPGKFPDYKEDHYNNHRLYYVTTKDFKKFSKAKVLI
DPDFSCIDATLVKRGKDDYVMVLKDNSRDQRNIKVAFAKSPYGPWSKASEPFTESFCEGP
STAMVNGWYYIYYDSYRHGIYGAARTKDFKNFQDQTGAVKFPVGHKHGTVFMADEEIVKN
LIKENRDAVHYTGTTVATPARHDGGLSPVVGVHSIQTMRGEQGWLYNHQPMMAYWKGKFY
MHYLTDPRSEHEPPGKTMLQTSADGYKWTKPVELFPEYNVPDGYTKPTWPGVVAKDLKAI
MHQRVGFYVSKSNRLIAMGNYGVALTPKDDPNDGNGIGRVVREIKADGTFGPIYFIYYNH
GFNEKNTDYPYYKKAKDKEFVKACDEILANPMYRMQWVEEADRNDDILPLKTPYKAFSGY
ILPDGRKVGLWKHALTSISSDGGNTWRLPVKRAHGFVNSNAKIWGQRLSDGTYATVYNPA
EYRWPLAISLSNDGLEYTTLNLVNGEVTPERHWGNYKSYGPQYTRGILEGNGTPPDNNLW
VTYSNNKEDMWVSCIPVPVKIKATEHAEGSFSKYAKLQDMKDWNIYSPLWAPVTLNGEWL
TLSDKDPYDYAKVEKLIPATKELTVDFDVKPAQNNHGQLNIEFLDDKGNMCSRIVLDSAA
TMKVKGGARYGGMLKHYDAGQTYHITAKLSVDGHVGTYFVNGKKVTSRMFDTPVDAITRI
VFRTGDLFTEPSIETPADQYVDMPRADEQDKLASFSIANVKTASADADSASTVLKYDNFA
HYAEYFNNMEDENIVTDIPNSKSSEWMSKNIPLFECPDKDMEEIYYFRWWSLRKHIKRTP
VGLGMTEFLVQRSYADKYNLIACAIGHHVMETRWLRDSTYLHQILNTWYHGNNGKAMTKM
NKFSSWNPAAVYEAYKVLGDTAFVMALKPSLEEEYARWKSTNRLKSGLYWQGDVQDGMEE
SISGGRRKQYARPTINSYMYGNAMALAELNKLAGDTKKAKEYTLEADTIKNLIQTKLWSG
KQSFFETMRGDTLAAVREAIGFIPWYFNLPDAKKYDEAWKQVEDEEGFSAPYGLTTAERR
HPEFRSHGVGKCEWDGAIWPFATSQTLTAMANYINTYPEPVVGDSTFFKQMKLYVESQHH
RGRPYIGEYLDEKNGAWLMGDRERSRYYNHSTFADLVITGIAGLRPQADGSIVVNPLIPE
GTWNYFCLDNISYRGNNIAIVYDKDGQRYHQGKGLMLFVNGKLAAKRDGLGKLTYKIQDK

Enzyme Prediction     

No EC number prediction in MGYG000004848_01129.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH143	303	842	1.6e-251	0.9910071942446043
GH142	905	1371	9.2e-211	0.9958246346555324
GH43	66	291	7.4e-81	0.9912663755458515

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08983	GH43_Bt3655-like	5.95e-73	57	303	8	260	Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 arabinofuranosidase Bt3655. This glycosyl hydrolase family 43 (GH43)-like family includes the characterized arabinofuranosidases (EC 3.2.1.55): Bacteroides thetaiotaomicron VPI-5482 (Bt3655;BT_3655) and Penicillium chrysogenum 31B Abf43B, as well as Bifidobacterium adolescentis ATCC 15703 beta-xylosidase (EC 3.2.1.37) BAD_1527. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd08978	GH_F	5.70e-11	69	257	1	192	Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
pfam13088	BNR_2	8.02e-08	540	614	168	247	BNR repeat-like domain. This family of proteins contains BNR-like repeats suggesting these proteins may act as sialidases.
cd08986	GH43-like	1.97e-07	68	227	2	218	Glycosyl hydrolase family 43 protein; uncharacterized. This glycosyl hydrolase family 43 (GH43)-like subfamily includes uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
COG3408	GDB1	1.93e-06	1030	1233	347	551	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT29071.1	0.0	306	1374	23	1100
ALJ45260.1	0.0	306	1374	23	1100
QRQ56889.1	0.0	306	1374	23	1100
SCV09596.1	0.0	306	1374	23	1100
QDH55638.1	0.0	306	1374	23	1100

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQR_A	0.0	306	1374	25	1102	SialidaseBT_1020 [Bacteroides thetaiotaomicron]
5MQS_A	0.0	306	1374	25	1102	SialidaseBT_1020 [Bacteroides thetaiotaomicron]
6M5A_A	5.28e-28	924	1375	303	811	Crystalstructure of GH121 beta-L-arabinobiosidase HypBA2 from Bifidobacterium longum [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH9	5.58e-27	924	1375	334	842	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000285	0.999029	0.000194	0.000187	0.000162	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004848_01129.