CAZyme Information: MGYG000004850_00244

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae
• CAZyme ID: MGYG000004850_00244
• CAZy Family: GH30
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1597	MGYG000004850_6|CGC1	172372.17	3.893

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004850	2046759	MAG	China	Asia

• Gene Location: Start: 14973; End: 19766 Strand: -

Full Sequence      

MDLKKAVSGVLAAAIVAGTATSSLPSVSAATEKAAVPTVSVDPTIRYQTLEGWGTSLAWF
ANIIGGWTEDYNGNGRPDREDIAELIYSPEYLNMNIVRYNIGGGDDPTHDHIKRCEATVP
GWKASADAELDPTADANQIWFLEQANEWRDDVINEVFSNTPPYYMTVSGCASGAVNANDN
NLRDDQYDDFAQYLAEVTQWLDNHLYENYGNHIDYIEPINEPDTNYWAAYSTKQEGCHFD
SGEKQSLIYRETLNALADLGLDHIQITGTDETSVDHAINSFNKLESDVKEAMPVISTHTY
GANNRQVLSDLAASYDKGLWMSEVCHGGGPHDPESMQATASFTFANSIKEDLKTLQSTAW
VDWQVVDSEYELLKWDQNWGLIHAVYEADDQPVPGYHDNLLDENGEKLDWVPGTGTWHIG
KQFYTLMQYSKYLKAGYTMIDIGNNDMVAAVSPNGDELVIVANNSGAAQQQYLNLSAFPG
AVKAEMYRTTDSESCELVNTLTVSGNVLPVELAQGSVTTFVITGKDGASLYDNENGTIKR
VNSGVVTNSDTAALGATTNNKFEYSGSWKRYTSQSGAYQADVQGTTTPGSSVTFRFDGDR
AVVTGNTEATGGKLTYTIDGGEAVALDTYTETKLYGQVLVDTGYLPEGSHTITITMTEGS
TGGTSSPIITVDEALVYSGEVKDSSTPPLVPGISAFDGSILIAFNPVEGAENYTIRYGTV
SDASQWTESITTETSPAVIKGLENGVPCYFTVQASIPGETTVPTAVFTATPNAVEDSNVY
YFVDSGVSNPFDVSTTSSGMYSSNLEQPYGEDPVTGKLWGYNADTASGTSAGMDAWDSVR
YDDQDTAGKGIQYTFELEPGEYTVEVGLYDPWQNGGRYQDVIVQGETVATRQLGITRNQI
VAKGVVAEGENAMTVEIVRSAGVTDQYQDPVVSYVKISEYDPDAIASINAPNAIISPAGQ
LPDMPEQVTVTTISGGERLANVTWEMSLDQFMTPYSVVMVTGTVEGVENKLTAKITVMPE
MPLYFVDSGIREGETSDLHNLVSSAFPELLNRDTPDQAYTEGSWGYIASDVGAYYNESGN
AYNNGWYAYSGKDITYKFQLPAGTYELNAGFHEWWNVARNMDVSVDYVDAEGNPQTTLVL
DNIFINGSSDQDIMSTGTFTLPADGEISVRVHKAPGEKNDPVVSWLAISCSEGALKELRN
LISEASVLNEDDYTPGTWAALQTALEAARTAASGIDSAAMAAAADELSAKLEALIPAADF
SELAKAIADAEKYAESDYTEESWGVFADALAKANEVNDNAEATQEDVDAAVKALNDAIAG
LEEYAAPNKTLLQKTYEHALTLSTEGITDSAKAYFEKVLAEAKAVLDDSKATQEEVDTAW
NNLLEGIWGLGITQGDKTMLEQLITKAEDMVANENKYVQINWQQLLDALEAAKAVMEDVD
ALTADVEAASEDLLNAILAQRFKADKSNLEDLISKAESIDLSKYTEESVAVFQAAFKAAN
AVLADESLSEDDQDVVDNAVSELNAAIENLSVKDDASDSDDGNDKDDSSKPDDNKNDTSS
TPSDPSETGDMTPYALAAAVVALSAAAMLVLKQKARI

Enzyme Prediction     

No EC number prediction in MGYG000004850_00244.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	39	520	4.3e-134	0.9978070175438597

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14587	Glyco_hydr_30_2	2.27e-23	38	367	3	357	O-Glycosyl hydrolase family 30.
pfam17996	CE2_N	3.37e-07	588	662	18	89	Carbohydrate esterase 2 N-terminal. This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum.
pfam07554	FIVAR	1.38e-05	1467	1530	1	68	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
pfam07554	FIVAR	1.00e-04	1261	1322	1	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
NF033441	BREX_BrxC	1.02e-04	1272	1529	833	1088	BREX system P-loop protein BrxC. BrxC is a P-loop-containing protein, and probable ATPase, from BREX (bacteriophage exclusion) systems of type 1.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL05173.1	8.25e-264	32	1278	37	1273
AWY99102.1	1.44e-231	39	1530	44	1287
QIB57138.1	5.75e-211	39	1505	43	1260
QMW80085.1	5.75e-211	39	1505	43	1260
ADU23821.1	7.73e-89	35	524	30	500

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q76FP5	2.36e-35	38	469	23	414	Endo-beta-1,6-galactanase OS=Hypocrea rufa OX=5547 GN=6GAL PE=1 SV=1
A0A401ETL2	1.40e-34	3	494	4	586	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1
E8MGH9	1.16e-07	1259	1461	1667	1876	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1
P26831	3.32e-07	1322	1574	1353	1617	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q9L7Q2	5.04e-06	1197	1383	420	617	Zinc metalloprotease ZmpB OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=zmpB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000388	0.998747	0.000194	0.000255	0.000204	0.000184

TMHMM  Annotations     

start	end
1574	1591