CAZyme Information: MGYG000004867_01255

Basic Information

• Species: HGM11616 sp900761075
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; Christensenellaceae; HGM11616; HGM11616 sp900761075
• CAZyme ID: MGYG000004867_01255
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1975		219066.23	4.2004

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004867	2137430	MAG	China	Asia

• Gene Location: Start: 784; End: 6711 Strand: -

Full Sequence      

MAKPEREKMKSIFKKLTALTLAVLLLAAISPNGLALIKIVSAQDVYGEEMLSNGSFEDEL
NGWEWNDGFSISNEAAAEGEKSFKYEVNDNNFAAAGHASIAVKQNTWYSLSASFYRSDEN
QWLYVDMSDIAGEAQVMAQGSGEWETQEGLWYSGDATSVAVRIVCEKNWTLGTTGKGDYY
IDAISLREVTREQREEMVYNGSFEHGNEEWNWIDGFDIVEDKASDGTHSIKWNGLSLSTA
GYTSVPVEKNTWYVLSADFFRSDDDRWVYVDMSDISGEATVSAAETGKWESQQVLWYSGD
NTAVSLRAVCEKNWTTGADGKGQYYIDNIAMYKKTVVTAAEFLDEKTISVTVEPAEKKKA
EDFSVTLGRETVEIAEVKQAQDGKMIITLAEPVIMGQTYTITGKNRLQGSAKAKYISADA
ITLKDAKFLDASTVEIEIDKSDKLLKEELSLEISVNYGEYRKLEEGAWSIADNAITLNTP
AEEGTMLRIKGNGKVLGSVSAQFGKMTFPNTIRHDDYGVSYLGDISQKSDYTLLNEPEAS
FEFTFVGTGIELYGPKGLGMGKADVLIDGILAGEIYSYSNQAISETKLFEKSGLEDGLHL
VTVVKNRKNNETYPVGFSKAQVKTGDEEGADKYSLNGQWSILKDGPGSSAWSDADGFDFE
NAEPILVPGNIWEAFPGYNGVSWYGKVFNDYLVSEENERMYLRFEAVQYSCDVYLNGEKL
GSHIGSEVPFEYDVTDIIRPNEENFIAVAVSNGKGMLLAGSNTETGAFWDCGGIWQEVSL
ISRPEVYISDVYAKPDWKTGEVEMDITVVNETEEQKTVSIGAAINEKGGAVVENIAAQSF
QAAPGENTFTIKGKVKNFKLWDTENPNLYEVNATLSSASGRHSYEPFHIGFRHIEIKDGY
YYLNGERFFLKMIHQNAYDPLIIQGTPRDLTYQTKALDNLKETGFNTFRTIGMAGLPAQL
DYADEIGLLIYEESSQSWLGKNAWINEVQDELIIRDRNHPSIIVWGLLNEIVYAGDNRLD
ITRDYLTRLRTLDMTRPVAWNSGSFDYNSLCSHMSNSYSTTWDIYMGNESETLEQNGYYD
GSLADTTIDSSKGYVGDLHFYPVYPLQKSSLEYLKTIGEGVNPVSITEAGTGSMFNPYNE
RDKTIAAGGDTSSFGMQKWVYPLIDGLDYFFNKYPTLSKLYDEPRNIIDDSDAINLRQRQ
ILMSYIRANPQVNGYGLTSLSDAQGLGEGVMDNFRDWKQGYDDMLKEAWAPLRWCILLNN
ENSNVKKGEYAEFEVDIANEDVLQAGEYTAVFEILDKSGKAVYQSEDITFSVEDGESAPL
AYEVWKGKVLIDFPEGEYTLKASLQSEDYAPTAEKMTFHVTDNSNVIDEDVKAVTVAGEI
PNSLKESLAENGVAIREYSAYAEIDNEVILVGESSPNTPGFWRSLYKKVAKGAYASILNA
EYLGENYQWFPAESASKPYEFSTAHLGSLYHNEWIAMEDEVLMDGLETGMVDPSFYGEKL
TKKGANQMAMPIMPDEVNMVSVYEQGLPLAGYVAEGVAIGTYQHHNGYFTLSYLDLANAA
DAPAVERLILNMVNYGSKRSTEVTRIDEEAYEDELNSYGFKEEIYKGFYLVNDDSPAIEY
GPESKPGNGGADYICTDETLIKDYLKFTFIGNKVVVYSAKNIDLGIADVYIDGVKVGQID
YYAQSIAFQQVVFESEKLTQGEHTLEIRHTGQKGDAATGTLTPIDAIEYASDLPQVYDIE
LSCDDVVFIGETCKATATVQPKDAQIGCAKWNSSNPNIATVSAAGEITPISTGKTVISVM
AADGSGTIAQKEITVLDKANLTVDIPVKAEWNLKDVDKMPEQVKIVLLANGEAIDELLLE
KANGYKGIFEDVLTYEDGKLVELTIRAQEIKGVNVEITGNAYEGFVAIYNAEPDNEPQKP
VKPSDSDKPSDDETIDAEGNQPETGDKNGLWLWVFVLMICAVSILAIGRRVYKKQ

Enzyme Prediction     

No EC number prediction in MGYG000004867_01255.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	623	1374	1.2e-82	0.7646276595744681

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.90e-45	634	1031	14	437	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	6.76e-33	633	1038	13	445	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.26e-32	617	1062	25	492	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	2.53e-25	626	1038	46	485	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	1.74e-15	634	784	3	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGQ94272.1	2.36e-152	632	1599	500	1453
AZN41345.1	4.16e-107	635	1586	14	989
QGQ94278.1	1.19e-104	635	1589	8	978
QBY04221.1	4.78e-41	634	1410	38	800
QLH78435.1	2.71e-39	634	1300	9	643

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6D89_A	3.89e-31	634	1038	38	440	Bacteroidesuniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_B Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_C Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_D Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis]
6D8K_A	1.66e-30	615	1036	18	438	Bacteroidesmultiple species beta-glucuronidase [Bacteroides ovatus],6D8K_B Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_C Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_D Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus]
6D1N_A	1.72e-29	634	1038	38	448	Apostructure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D1N_B Apo structure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D41_A Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D41_B Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D6W_A Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_B Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_C Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_D Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D7F_A Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_B Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_C Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_D Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_E Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_F Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis]
7VQM_A	3.02e-28	657	1039	68	437	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]
6ED1_A	1.57e-27	627	1036	21	447	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	2.13e-31	661	1032	78	456	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	8.12e-31	629	1037	2	411	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P23989	3.17e-26	679	1118	119	552	Beta-galactosidase OS=Streptococcus thermophilus OX=1308 GN=lacZ PE=3 SV=1
Q6LL68	3.18e-26	630	1043	44	484	Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1
B4S2K9	3.22e-26	630	1046	45	491	Beta-galactosidase OS=Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype) OX=1774373 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.076365	0.921786	0.001087	0.000280	0.000220	0.000225

TMHMM  Annotations     

start	end
21	43
1949	1968